Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,161,578	A→G	I364I (ATT→ATC)	ftsP ←	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,161,578	0	A	G	100.0%	183.9 / NA	51	I364I (ATT→ATC)	ftsP	septal ring component that protects the divisome from stress, multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/25);  total (26/25)

ACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCA  >  NC_000913/3161452‑3161711                                                                                                                               |                                                                                                                                      aCGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtca                                                                                                                           >  2:405538/1‑139 (MQ=255) aCGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtca                                                                                                                           <  2:1772731/139‑1 (MQ=255)        tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTTGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAcc                                                                                                                                  >  1:1612363/1‑125 (MQ=255)        tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTTGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAcc                                                                                                                                  <  2:1612363/125‑1 (MQ=255)        tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAccc                                                                                                                                 <  1:619657/126‑1 (MQ=255)        tGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATAccc                                                                                                                                 >  2:619657/1‑126 (MQ=255)            aCATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACt                                                                                                                <  2:1582082/139‑1 (MQ=255)                  cGCCTTCAATATGGAACGCATGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATc                                                                                                          >  1:253483/1‑139 (MQ=255)                               gAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAAtt                                                                                             >  2:180672/1‑139 (MQ=255)                                  cGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGc                                                                                          >  1:1507544/1‑139 (MQ=255)                                   gCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtc                                                                                                                            >  1:241901/1‑104 (MQ=255)                                   gCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGtc                                                                                                                            <  2:241901/104‑1 (MQ=255)                                     ttGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGaa                                                                                       >  2:1786334/1‑139 (MQ=255)                                     ttGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGaa                                                                                       >  1:497944/1‑139 (MQ=255)                                       gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAc                                                                                                                       >  2:1736455/1‑105 (MQ=255)                                       gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAc                                                                                                                       <  1:1736455/105‑1 (MQ=255)                                       gCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAAcc                                                                                     >  2:1251382/1‑139 (MQ=255)                                          gCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGc                                                                                  <  2:1113289/139‑1 (MQ=255)                                                                gCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAg                                                            >  2:1377031/1‑139 (MQ=255)                                                                  gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:279651/139‑1 (MQ=255)                                                                  gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:405538/139‑1 (MQ=255)                                                                  gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:1251382/139‑1 (MQ=255)                                                                  gTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  1:152656/139‑1 (MQ=255)                                                                    tCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCa                                                        <  2:194766/139‑1 (MQ=255)                                                                      ccACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATc                                                      >  2:597856/1‑139 (MQ=255)                                                                            tCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGa                                                <  2:1229373/139‑1 (MQ=255)                                                                             ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGcc                                                                                 >  2:1597671/1‑105 (MQ=255)                                                                             ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGcc                                                                                 <  1:1597671/105‑1 (MQ=255)                                                                             ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  1:180672/139‑1 (MQ=255)                                                                             ccTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  2:754297/139‑1 (MQ=255)                                                                              cTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAcc                                                                                                                      >  2:692424/1‑67 (MQ=255)                                                                              cTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCAcc                                                                                                                      <  1:692424/67‑1 (MQ=255)                                                                                            aCATCAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCg                                >  2:1008984/1‑139 (MQ=255)                                                                                                cAATACGGTTGACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAg                            <  2:253483/139‑1 (MQ=255)                                                                                                          gACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                       <  1:578964/134‑1 (MQ=255)                                                                                                          gACGTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGc                       >  2:578964/1‑134 (MQ=255)                                                                                                             gTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               <  1:244219/107‑1 (MQ=255)                                                                                                             gTCCCACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGAc                                               >  2:244219/1‑107 (MQ=255)                                                                                                               cccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcg             <  1:1786334/139‑1 (MQ=255)                                                                                                                ccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcgc            <  2:938024/139‑1 (MQ=255)                                                                                                                ccACAGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGgcgc            >  1:392364/1‑139 (MQ=255)                                                                                                                  acaGCTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAg          >  1:674363/1‑139 (MQ=255)                                                                                                                      cTGTCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGtt      >  1:882860/1‑139 (MQ=255)                                                                                                                         tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          >  1:904386/1‑84 (MQ=255)                                                                                                                         tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  2:904386/84‑1 (MQ=255)                                                                                                                         tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGc   >  2:1373247/1‑139 (MQ=255)                                                                                                                         tCCATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGc   >  1:56784/1‑139 (MQ=255)                                                                                                                          ccATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCa  >  1:352456/1‑139 (MQ=255)                                                                                                                          ccATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCa  <  2:56784/139‑1 (MQ=255)                                                                                                                           cATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          <  2:63123/82‑1 (MQ=255)                                                                                                                           cATTGATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAgcg                                                          >  1:63123/1‑82 (MQ=255)                                                                                                                               |                                                                                                                                      ACGGATCTGGAACATTACGCCTTCAATATGGAACGCTTGCGGCTCGTCCGCGCGTACCGTCCAGCGTTCCCACGTTCCTTGCTGCGCGGTGACATCAATACGGTTGACGTCCCACAGCTGTCCATTAATACCCGGGTCATCACCCAGACTGATATCGCGACTGCGAATTGGCGAACCAGCCATGATTTCAGTTGGCAGCAAGCGCATCGGAAGACTGTCTGTGACCAGCGGCAGAAGGCCGGTTGGGCGCAGCGTTAGCA  >  NC_000913/3161452‑3161711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑