Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,205,324	C→T	*311* (TAG→TAA)	ttdR ←	transcriptional activator of ttdABT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,205,324	0	C	T	100.0%	208.1 / NA	65	*311* (TAG→TAA)	ttdR	transcriptional activator of ttdABT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/32);  total (33/32)

TTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAA‑CAGCCCAGATATTGGCGCTT  >  NC_000913/3205191‑3205456                                                                                                                                      |                                                                                                                                      ttCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACAt                                                                                                                                  <  1:578/139‑1 (MQ=255)     aTGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGAcc                                                                                                                              >  1:62892/1‑139 (MQ=255)                   gATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATc                                                                                                                >  2:89826/1‑139 (MQ=255)                    aTCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCg                                                                                                               >  1:228530/1‑139 (MQ=255)                     tCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCgg                                                                                                              >  2:538773/1‑139 (MQ=255)                      ccTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCggg                                                                                                             <  2:1374137/139‑1 (MQ=255)                         gctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCACGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGcc                                                                                                                      <  2:796568/127‑1 (MQ=255)                         gctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCACGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGcc                                                                                                                      >  1:796568/1‑127 (MQ=255)                          ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGAcc                                                                                                                              <  1:999026/118‑1 (MQ=255)                          ctgcGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGAcc                                                                                                                              >  2:999026/1‑118 (MQ=255)                               tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                    >  1:1252662/1‑139 (MQ=255)                               tcatcaTGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAg                                                                                                    >  2:995647/1‑139 (MQ=255)                                 atcatGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                                  <  2:62892/139‑1 (MQ=255)                                       acaacaTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCt                                                                                            >  2:94150/1‑139 (MQ=255)                                       acaacaTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCt                                                                                            >  1:1249803/1‑139 (MQ=255)                                            aTCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAc                                                                                       >  1:1509109/1‑139 (MQ=255)                                             tCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCAcc                                                                                      >  1:208526/1‑139 (MQ=255)                                               cAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCAt                                                                                    <  2:228530/139‑1 (MQ=255)                                                aaCGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTTATAGcc                                                                                                                      >  1:351488/1‑104 (MQ=255)                                                aaCGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGcc                                                                                                                      <  2:351488/104‑1 (MQ=255)                                                aaCGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATg                                                                                   >  2:1178997/1‑139 (MQ=255)                                                      tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGa                                                                                                                                <  1:206224/88‑1 (MQ=255)                                                      tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGa                                                                                                                                >  2:206224/1‑88 (MQ=255)                                                      tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                                  >  1:296008/1‑118 (MQ=255)                                                      tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGcc                                                                                                  <  2:296008/118‑1 (MQ=255)                                                      tgtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCa                                                                             >  2:1404662/1‑139 (MQ=255)                                                       gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAg                                                                            >  1:39014/1‑139 (MQ=255)                                                       gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAg                                                                            >  1:378216/1‑139 (MQ=255)                                                       gtgGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAg                                                                            >  2:689861/1‑139 (MQ=255)                                                             tcgtcAGGAGACAAAAATCTGGACGAAATTCAAAATAAAGCGCGAAGAGGATCCCGAGTGATTTCTGGTGGATTAACATGACCTGATAGCCTTCATCGTGCTTGCGCAGCCGTCGCTGGCACCATGCCGCCAGAAATTc                                                                      >  1:1577082/1‑139 (MQ=255)                                                               gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGcc                                                                                                                      <  1:1425400/89‑1 (MQ=255)                                                               gtcAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGcc                                                                                                                      >  2:1425400/1‑89 (MQ=255)                                                                   ggAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCa                                                                <  2:1252662/139‑1 (MQ=255)                                                                    gagaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAg                                                               >  2:1226211/1‑139 (MQ=255)                                                                    gagaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAg                                                               <  1:1038580/139‑1 (MQ=255)                                                                     agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGATCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                              <  1:1226211/139‑1 (MQ=255)                                                                     agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                              <  2:1286273/139‑1 (MQ=255)                                                                     agaCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                              <  2:905760/139‑1 (MQ=255)                                                                      gaCAAAAATCTGGACGAAATTCAAGAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGac                                                             <  1:94150/139‑1 (MQ=255)                                                                       aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACg                                                                  >  1:255983/1‑133 (MQ=255)                                                                       aCAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACg                                                                  <  2:255983/133‑1 (MQ=255)                                                                                 ggACGAAATTCAAAAGAAAGCGCGACAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCa                                                  <  1:541387/139‑1 (MQ=255)                                                                                 ggACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCa                                                  <  2:39014/139‑1 (MQ=255)                                                                                 ggACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCa                                                  <  2:378216/139‑1 (MQ=255)                                                                                                 aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                              <  2:822019/111‑1 (MQ=255)                                                                                                 aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGa                                                              >  1:822019/1‑111 (MQ=255)                                                                                                 aaagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCgg                                  >  2:559883/1‑139 (MQ=255)                                                                                                  aagCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGc                                 <  1:689861/139‑1 (MQ=255)                                                                                                   agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTg                                                                                           <  1:1277602/80‑1 (MQ=255)                                                                                                   agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTg                                                                                           >  2:1277602/1‑80 (MQ=255)                                                                                                   agCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCt                                >  2:202868/1‑139 (MQ=255)                                                                                                            aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                       <  1:1404662/139‑1 (MQ=255)                                                                                                            aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGCaaa                       <  1:1178997/139‑1 (MQ=255)                                                                                                            aGGATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAACCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                       <  1:538773/139‑1 (MQ=255)                                                                                                             ggATCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAA‑c                     <  2:1249803/139‑1 (MQ=255)                                                                                                               aTCCCGAGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGtt                                                                                               >  2:1036881/1‑64 (MQ=255)                                                                                                               aTCCCGAGTGATTTCTGGAGGATTTACATGACCTTATAGCCTTCATCGGGCTTGCCCAGCCGtt                                                                                               <  1:1036881/64‑1 (MQ=255)                                                                                                                     aGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAAAGCGGc                                 <  2:238373/120‑1 (MQ=255)                                                                                                                     aGTGATTTCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAAAGCGGc                                 >  1:238373/1‑120 (MQ=255)                                                                                                                          tttCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                          >  1:613514/1‑90 (MQ=255)                                                                                                                          tttCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACg                                                          <  2:613514/90‑1 (MQ=255)                                                                                                                            tCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                       <  2:1781805/123‑1 (MQ=255)                                                                                                                            tCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTaaa                       >  1:1781805/1‑123 (MQ=255)                                                                                                                            tCTGGTGGATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAACCAGCCCAGATATTgg       >  1:1632443/1‑139 (MQ=255)                                                                                                                                gtggATTTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAA‑CAGCCCAGATATTGGCGCtt  <  1:89826/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      TTCGATGCTCTCTTGCCTGATCCTGCTGCGTCATCATGACAACATCCAACGTCTGTGGCGTCGTCAGGAGACAAAAATCTGGACGAAATTCAAAAGAAAGCGCGAAAAGGATCCCGAGTGATTTCTGGTGGATCTACATGACCTGATAGCCTTCATCGGGCTTGCCCAGCCGTTGCTGGCACCATGCCGCCAGAAATTCCACGCAGACACGTAATTTCATGCTGCGATAGAGCGGCTCCCGGTAAA‑CAGCCCAGATATTGGCGCTT  >  NC_000913/3205191‑3205456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑