Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,220,190 G→A A233T (GCT→ACT)  patA → putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,220,1900GA100.0% 191.3 / NA 61A233T (GCT→ACT) patAputrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/35);  total (26/35)

GTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCA  >  NC_000913/3220057‑3220322
                                                                                                                                     |                                                                                                                                    
gTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCg                                                                                                                                 >  1:1724720/1‑139 (MQ=255)
  cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                               <  1:109693/139‑1 (MQ=255)
  cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                               <  1:849541/139‑1 (MQ=255)
  cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                               <  1:608418/139‑1 (MQ=255)
  cGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGt                                                                                                                               <  1:1574359/139‑1 (MQ=255)
            gAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                     <  1:1133274/139‑1 (MQ=255)
                  gACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                               <  2:1008251/139‑1 (MQ=255)
                   aCCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTc                                                                                                              >  2:1128537/1‑139 (MQ=255)
                          gCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGa                                                                                                       <  2:318461/139‑1 (MQ=255)
                            aaaCCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAA‑CGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                    <  1:1353201/139‑1 (MQ=255)
                             aaCCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                    <  2:1724720/139‑1 (MQ=255)
                                  tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                               >  1:1350637/1‑139 (MQ=255)
                                  tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                               >  1:1515151/1‑139 (MQ=255)
                                  tttATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCg                                                                                               >  1:1618702/1‑139 (MQ=255)
                                            tACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACCGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgcc                                                                                     >  1:1421289/1‑139 (MQ=255)
                                                gCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccg                                                                                 <  2:1515151/139‑1 (MQ=255)
                                                 ccGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                               >  2:505133/1‑108 (MQ=255)
                                                 ccGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGAtt                                                                                                               <  1:505133/108‑1 (MQ=255)
                                                 ccGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTgccgccgc                                                                                >  1:1568708/1‑139 (MQ=255)
                                                      cTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAATGCAAAAAAACCGGTGATGATGTGACTGCAGTGATCCTCGAACCTATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGc                                                                           <  1:1184650/139‑1 (MQ=255)
                                                            tCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTAtctc                                                                     <  1:1120282/139‑1 (MQ=255)
                                                                gtgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccg                                                                 >  2:780567/1‑139 (MQ=255)
                                                                gtgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccg                                                                 <  2:1568708/139‑1 (MQ=255)
                                                                 tgCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                >  1:1698354/1‑139 (MQ=255)
                                                                   ccGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgccg                                                              <  2:1302494/139‑1 (MQ=255)
                                                                      tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGTGGTGATCCt                                                                                                                         <  2:1632152/77‑1 (MQ=255)
                                                                      tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGTGGTGATCCt                                                                                                                         >  1:1632152/1‑77 (MQ=255)
                                                                      tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                     >  2:1731286/1‑81 (MQ=255)
                                                                      tttGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                     <  1:1731286/81‑1 (MQ=255)
                                                                         ggCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGCTGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCcgtacgta                                                        >  1:1625252/1‑139 (MQ=255)
                                                                                tCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTg                                                                                                                              >  2:1615036/1‑62 (MQ=255)
                                                                                tCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTg                                                                                                                              <  1:1615036/62‑1 (MQ=255)
                                                                                   aaGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCa                                                                    >  2:813244/1‑117 (MQ=255)
                                                                                   aaGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCa                                                                    <  1:813244/117‑1 (MQ=255)
                                                                                    aGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGAt                                             >  2:368046/1‑139 (MQ=255)
                                                                                       cATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAg                                          >  1:1213975/1‑139 (MQ=255)
                                                                                         tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGTGGTGATCCTCGaa                                                                                                                     <  2:1054172/62‑1 (MQ=255)
                                                                                         tGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGaa                                                                                                                     >  1:1054172/1‑62 (MQ=255)
                                                                                          gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAAc                                                                                                                    >  2:1795401/1‑62 (MQ=255)
                                                                                          gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAAc                                                                                                                    <  1:1795401/62‑1 (MQ=255)
                                                                                          gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                <  1:8209/114‑1 (MQ=255)
                                                                                          gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                >  2:8209/1‑114 (MQ=255)
                                                                                          gcgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTc                                       >  2:1766159/1‑139 (MQ=255)
                                                                                           cgcACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACTGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCg                                      <  2:1107667/139‑1 (MQ=255)
                                                                                              aCGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGgcg                                   <  1:182352/139‑1 (MQ=255)
                                                                                                ggCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                >  1:1101739/1‑108 (MQ=255)
                                                                                                ggCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                                 <  1:780567/139‑1 (MQ=255)
                                                                                                ggCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGACCGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                                 <  2:1421289/139‑1 (MQ=255)
                                                                                                ggCTCTTAACGAGTGCAAAAAAACCGGTGATCATCTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCAccgc                                                                <  2:1101739/108‑1 (MQ=255)
                                                                                                    ctTAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACtga                             <  2:1318892/139‑1 (MQ=255)
                                                                                                     ttAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                                 >  1:1180001/1‑134 (MQ=255)
                                                                                                     ttAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCa                                 <  2:1180001/134‑1 (MQ=255)
                                                                                                      tAACGAGTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACtgatg                           <  2:1698354/139‑1 (MQ=255)
                                                                                                            gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCt                                                                                                                         <  1:1735437/39‑1 (MQ=255)
                                                                                                            gTGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCt                                                                                                                         >  2:1735437/1‑39 (MQ=255)
                                                                                                             tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                    <  1:554063/59‑1 (MQ=255)
                                                                                                             tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAgg                                                                                                    >  2:554063/1‑59 (MQ=255)
                                                                                                             tGCAAAAAAACCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCg                    <  2:1625252/139‑1 (MQ=255)
                                                                                                                aaaaaaaCCGGTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATg                 <  1:1128537/139‑1 (MQ=255)
                                                                                                                         ggTGATGATGTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACaaa        >  2:1669496/1‑139 (MQ=255)
                                                                                                                               gatgTGACTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCa  <  1:368046/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GTCGGCCACGGCGAAATCGACCTTCCGCAAACCGTTTATGCCGTTACTGCCGGGCTTCCGTCATGTGCCGTTTGGCAATATCGAAGCCATGCGCACGGCTCTTAACGAGTGCAAAAAAACCGGTGATGATGTGGCTGCGGTGATCCTCGAACCGATTCAGGGTGAAGGTGGCGTAATTCTGCCGCCGCCGGGCTATCTCACCGCCGTACGTAAGCTATGCGATGAGTTCGGCGCACTGATGATCCTCGATGAAGTACAAACGGGCA  >  NC_000913/3220057‑3220322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑