Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,223,149 T→C L173L (TTG→CTG)  ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,223,1490TC100.0% 310.8 / NA 84L173L (TTG→CTG) ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/44);  total (40/44)

ACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGT  >  NC_000913/3223014‑3223279
                                                                                                                                       |                                                                                                                                  
aCGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCtgctgt                                                                                                                                 >  2:393943/1‑139 (MQ=255)
 cGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtg                                                                                                                                >  1:1723445/1‑139 (MQ=255)
  gCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                               >  1:971730/1‑139 (MQ=255)
  gCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                               >  1:405559/1‑139 (MQ=255)
   cTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgtg                                                                                                                              >  1:1390740/1‑139 (MQ=255)
      aTTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTgc                                                                                                                           <  2:405559/139‑1 (MQ=255)
                aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCa                                                                                                                 <  1:1475015/139‑1 (MQ=255)
                aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTAGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGc                                                                                                                  >  1:1088910/1‑138 (MQ=255)
                aCGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTAGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGc                                                                                                                  <  2:1088910/138‑1 (MQ=255)
                    cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTgg                                                                                                             >  2:781289/1‑139 (MQ=255)
                    cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTgg                                                                                                             >  2:1216967/1‑139 (MQ=255)
                    cGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTgg                                                                                                             >  1:889922/1‑139 (MQ=255)
                     gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            <  2:971730/139‑1 (MQ=255)
                     gTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            <  1:1403003/139‑1 (MQ=255)
                       cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                          <  2:941166/139‑1 (MQ=255)
                       cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                          <  2:1723445/139‑1 (MQ=255)
                       cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                          <  2:1649703/139‑1 (MQ=255)
                       cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                          <  2:735441/139‑1 (MQ=255)
                       cGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCg                                                                                                          <  1:256686/139‑1 (MQ=255)
                               aTTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTctac                                                                                                  <  2:640520/139‑1 (MQ=255)
                               aTTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTctac                                                                                                  <  2:1074398/139‑1 (MQ=255)
                                ttttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTctacc                                                                                                 <  2:1390740/139‑1 (MQ=255)
                                 tttGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTctacct                                                                                                >  1:1547724/1‑139 (MQ=255)
                                    gAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACg                                                                                             <  2:1276871/139‑1 (MQ=255)
                                       gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACctgctg                                                                                                                                  >  1:1775413/1‑99 (MQ=255)
                                       gTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                          <  2:889922/139‑1 (MQ=255)
                                          tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGAc                                                                                                       >  2:513844/1‑123 (MQ=255)
                                          tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGAc                                                                                                       <  1:513844/123‑1 (MQ=255)
                                          tATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAg                                                                                       >  2:1733958/1‑139 (MQ=255)
                                             gTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    <  1:1780710/139‑1 (MQ=255)
                                             gTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    <  2:421274/139‑1 (MQ=255)
                                                aaCGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                                 <  1:781289/139‑1 (MQ=255)
                                                  cGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                               <  1:555546/139‑1 (MQ=255)
                                                    gTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGatat                                                                             >  1:229101/1‑139 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATg                                                                                                                   <  1:535782/98‑1 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATg                                                                                                                   >  2:535782/1‑98 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGc                                                                                                                  >  1:654106/1‑99 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGc                                                                                                                  <  2:654106/99‑1 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGActct                                                                                                    <  1:828548/113‑1 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGActct                                                                                                    >  2:828548/1‑113 (MQ=255)
                                                       aGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATAtgtg                                                                          >  2:1545919/1‑139 (MQ=255)
                                                         tATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGaa                                                                                        <  1:1259229/123‑1 (MQ=255)
                                                         tATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGaa                                                                                        >  2:1259229/1‑123 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                          <  1:707073/115‑1 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                          >  2:707073/1‑115 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGa                                                                                         >  1:1527641/1‑116 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGa                                                                                         <  2:1527641/116‑1 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCa                                                                                   >  2:267408/1‑122 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCa                                                                                   <  1:267408/122‑1 (MQ=255)
                                                               ggTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                  >  2:1284781/1‑139 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                               >  1:1678149/1‑74 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCtgtgt                                                                                                                               <  2:1678149/74‑1 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCa                                                                                                                 <  2:1309851/88‑1 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCa                                                                                                                 >  1:1309851/1‑88 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                                 <  2:1311487/120‑1 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAgg                                                                                 >  1:1311487/1‑120 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                  <  2:1246873/135‑1 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAg                                                                  >  1:1246873/1‑135 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                              >  2:1194470/1‑139 (MQ=255)
                                                                   tCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCggg                                                              >  1:1393177/1‑139 (MQ=255)
                                                                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTg                                                                                           >  2:1191671/1‑108 (MQ=255)
                                                                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTg                                                                                           <  1:1191671/108‑1 (MQ=255)
                                                                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    <  2:1297630/115‑1 (MQ=255)
                                                                     aGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    >  1:1297630/1‑115 (MQ=255)
                                                                            gCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCcg                                                     <  1:1284781/139‑1 (MQ=255)
                                                                               gTCGCCTGACCGCAGAGTTTGACATCAGCTCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                                  <  2:1216733/139‑1 (MQ=255)
                                                                               gTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGa                                                  >  2:585601/1‑139 (MQ=255)
                                                                                tCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTg                                                                                                                      >  2:512331/1‑70 (MQ=255)
                                                                                tCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTg                                                                                                                      <  1:512331/70‑1 (MQ=255)
                                                                                     tGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            >  2:494369/1‑75 (MQ=255)
                                                                                     tGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            <  1:494369/75‑1 (MQ=255)
                                                                                      gACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTAt                                           <  2:1547724/139‑1 (MQ=255)
                                                                                            agagTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTACTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTc                                     >  1:893099/1‑139 (MQ=255)
                                                                                                 ttGACATCAGCGCGATGGTTAAAACCGGCGACAACCTACTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGaaa                                <  2:893099/139‑1 (MQ=255)
                                                                                                        cAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                          >  1:59969/1‑74 (MQ=255)
                                                                                                        cAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTgg                                                                                          <  2:59969/74‑1 (MQ=255)
                                                                                                        cAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTa                         >  1:1478649/1‑139 (MQ=255)
                                                                                                        cAGCGCGATGGTTAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTa                         >  1:873990/1‑139 (MQ=255)
                                                                                                                   ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAacac                            <  1:585554/125‑1 (MQ=255)
                                                                                                                   ttAAAACCGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAacac                            >  2:585554/1‑125 (MQ=255)
                                                                                                                          cGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                               >  1:1790057/1‑67 (MQ=255)
                                                                                                                          cGGCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGat                                                                               <  2:1790057/67‑1 (MQ=255)
                                                                                                                            gCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    <  1:445825/60‑1 (MQ=255)
                                                                                                                            gCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGAcc                                                                                    >  2:445825/1‑60 (MQ=255)
                                                                                                                            gCGACAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCAc     >  1:1180511/1‑139 (MQ=255)
                                                                                                                             cGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            <  2:548105/35‑1 (MQ=255)
                                                                                                                             cGACAACCTGCTGTGTGTGCGCGTGATGCAGTggg                                                                                                            >  1:548105/1‑35 (MQ=255)
                                                                                                                               aCAACCTGCTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACTTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACtgt  <  1:393943/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                  
ACGCTGATTAAATTTGACGGCGTCGAAACCTATTTTGAAGTCTATGTTAACGGTCAGTATGTGGGTTTCAGCAAGGGCAGTCGCCTGACCGCAGAGTTTGACATCAGCGCGATGGTTAAAACCGGCGACAACCTGTTGTGTGTGCGCGTGATGCAGTGGGCGGACTCTACCTACGTGGAAGACCAGGATATGTGGTGGTCAGCGGGGATCTTCCGCGATGTTTATCTGGTCGGAAAACACCTAACGCATATTAACGATTTCACTGT  >  NC_000913/3223014‑3223279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: