Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,301,599 G→A G39S (GGC→AGC)  yhbU → U32 peptidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,301,5990GA100.0% 167.7 / NA 52G39S (GGC→AGC) yhbUU32 peptidase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/22);  total (30/22)

CAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATGG  >  NC_000913/3301467‑3301734
                                                                                                                                    |                                                                                                                                       
cAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTa                                                                                                                                   >  1:1505680/1‑139 (MQ=255)
        aGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTAcc                                                                                                                           >  1:1425199/1‑139 (MQ=255)
                     gAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGc                                                                                                              <  2:1481523/139‑1 (MQ=255)
                               gccctgccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                                    <  2:1505680/139‑1 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTAc                                                                                                                            <  2:1246605/109‑1 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTAc                                                                                                                            >  1:1246605/1‑109 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCg                                                                                                      <  1:21167/131‑1 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCg                                                                                                      >  2:21167/1‑131 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                                    >  1:926580/1‑133 (MQ=255)
                                     ccGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGt                                                                                                    <  2:926580/133‑1 (MQ=255)
                                                        tAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATcgccgc                                                                           >  1:292866/1‑139 (MQ=255)
                                                                       aaaCGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg                                                            <  2:1425199/139‑1 (MQ=255)
                                                                          cGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                       >  2:1193073/1‑61 (MQ=255)
                                                                             cgcAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAac                                                      >  1:29274/1‑139 (MQ=255)
                                                                                         ttATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCAt                                          <  2:292866/139‑1 (MQ=255)
                                                                                                 ggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                       <  2:323479/38‑1 (MQ=255)
                                                                                                 ggCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGc                                                                                                                                       >  1:323479/1‑38 (MQ=255)
                                                                                                  gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAg                                                                                                 >  2:11813/1‑75 (MQ=255)
                                                                                                  gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAg                                                                                                 <  1:11813/75‑1 (MQ=255)
                                                                                                  gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACAt                                                                <  1:820076/108‑1 (MQ=255)
                                                                                                  gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACAt                                                                >  2:820076/1‑108 (MQ=255)
                                                                                                  gCTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGt                                 >  2:1186311/1‑139 (MQ=255)
                                                                                                   cTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATc                                                               <  2:1794753/108‑1 (MQ=255)
                                                                                                   cTAAAAGATGATACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATc                                                               >  1:1794753/1‑108 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTGCCGAGAAAAAGTTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCTGACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGtt                       >  2:1141354/1‑139 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAGAAATTGCAGGa                                                                                                          >  1:1269941/1‑56 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGa                                                                                                          <  2:1269941/56‑1 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAg                                                                                                 >  2:591558/1‑65 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAg                                                                                                 <  1:591558/65‑1 (MQ=255)
                                                                                                            gatACCAATGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGtt                       >  2:69563/1‑139 (MQ=255)
                                                                                                                  aaTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgc                 <  1:476014/139‑1 (MQ=255)
                                                                                                                   aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                             >  2:1304771/1‑62 (MQ=255)
                                                                                                                   aTGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGtttt                                                                                             <  1:1304771/62‑1 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCg                                                                                                      <  1:1738686/52‑1 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCg                                                                                                      >  2:1738686/1‑52 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg                                                            <  2:1498410/94‑1 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATcgcg                                                            >  1:1498410/1‑94 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGTGCATCCGGACGGTTACGCCCGTTGGCAgcgc               >  2:1598022/1‑139 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc               >  2:1253291/1‑139 (MQ=255)
                                                                                                                    tGCCCGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAgcgc               >  2:1436275/1‑139 (MQ=255)
                                                                                                                       ccGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGcc            <  1:1186311/139‑1 (MQ=255)
                                                                                                                        cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACtttt                                                <  1:1164443/102‑1 (MQ=255)
                                                                                                                        cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACtttt                                                >  2:1164443/1‑102 (MQ=255)
                                                                                                                        cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg                                       <  2:1789832/111‑1 (MQ=255)
                                                                                                                        cGTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCg                                       >  1:1789832/1‑111 (MQ=255)
                                                                                                                         gTCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGt          >  2:1378679/1‑139 (MQ=255)
                                                                                                                          tCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCa                   <  2:15977/129‑1 (MQ=255)
                                                                                                                          tCACTTCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCa                   >  1:15977/1‑129 (MQ=255)
                                                                                                                              ttCGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGata     >  2:706071/1‑139 (MQ=255)
                                                                                                                                cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATg   >  1:1661110/1‑139 (MQ=255)
                                                                                                                                cGCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATg   >  2:1157234/1‑139 (MQ=255)
                                                                                                                                 gCCAGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATgg  <  2:1661110/139‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
CAACCTTCAGGAAAAATTATGGAGCTGCTCTGCCCTGCCGGAAATCTCCCGGCGCTTAAGGCGGCCATCGAAAACGGCGCAGATGCTGTTTATATCGGGCTAAAAGATGATACCAATGCCCGTCACTTCGCCGGCCTTAACTTTACCGAGAAAAAATTGCAGGAAGCGGTGAGTTTTGTCCATCAACATCGCCGCAAACTTCACATCGCGATTAACACTTTTGCGCATCCGGACGGTTACGCCCGTTGGCAGCGCGCCGTGGATATGG  >  NC_000913/3301467‑3301734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑