Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,121	C→T	*310* (TAG→TAA)	yhcC ←	putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,121	0	C	T	100.0%	318.8 / NA	97	*310* (TAG→TAA)	yhcC	putative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (49/48);  total (49/48)

GTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352987‑3353248                                                                                                                                        |                                                                                                                                gTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             <  1:774820/139‑1 (MQ=255)          ataCTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  2:1047308/139‑1 (MQ=255)                cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGc                                                                                                               >  1:381635/1‑139 (MQ=255)                cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  1:1163085/1‑139 (MQ=255)                     gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGtt                                                                                                                        >  1:1103153/1‑124 (MQ=255)                     gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  2:806445/1‑139 (MQ=255)                     gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAAGAATACCCAATTCTGACAGTTACGTTATAAATGGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGtc                                                                                                                        <  2:1103153/124‑2 (MQ=255)                          cGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTcc                                                                                                    <  2:1163085/139‑1 (MQ=255)                                tGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  2:1679535/1‑128 (MQ=255)                                tGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  1:1679535/128‑1 (MQ=255)                                 gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:512097/139‑1 (MQ=255)                                    tCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:33290/139‑1 (MQ=255)                                    tCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:806445/139‑1 (MQ=255)                                               ttCCTTCACGACAACCTTCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTTA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                               <  2:1106304/139‑1 (MQ=255)                                               ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                <  2:381635/139‑1 (MQ=255)                                                  cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                            <  1:715165/139‑1 (MQ=255)                                                    tCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATcc                                                                                        <  1:1652452/125‑1 (MQ=255)                                                    tCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATcc                                                                                        >  2:1652452/1‑125 (MQ=255)                                                      aCGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCAt                                                                             >  2:1184958/1‑134 (MQ=255)                                                      aCGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCAt                                                                             <  1:1184958/134‑1 (MQ=255)                                                          cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  2:1164505/1‑102 (MQ=255)                                                          cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  1:1164505/102‑1 (MQ=255)                                                            aCCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                   >  2:789339/1‑90 (MQ=255)                                                            aCCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                   <  1:789339/90‑1 (MQ=255)                                                            aCCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:1766336/1‑112 (MQ=255)                                                            aCCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:1766336/112‑1 (MQ=255)                                                               tGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAg                                                               <  1:74882/139‑1 (MQ=255)                                                                  cccAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                            >  2:736920/1‑123 (MQ=255)                                                                  cccAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                            <  1:736920/123‑1 (MQ=255)                                                                           aCCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:1743135/100‑1 (MQ=255)                                                                           aCCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          >  2:1743135/1‑100 (MQ=255)                                                                           aCCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcat                                                                       >  2:1731851/1‑119 (MQ=255)                                                                           aCCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcat                                                                       <  1:1731851/119‑1 (MQ=255)                                                                           aCCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCg                                                   >  2:1400557/1‑139 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  2:1758467/81‑1 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  2:255825/1‑81 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  1:255825/81‑1 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  1:1758467/1‑81 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                            >  1:99111/1‑110 (MQ=255)                                                                               aaTTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATg                                                                            <  2:99111/110‑1 (MQ=255)                                                                                 ttCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcatt                                                                      <  2:1396860/114‑1 (MQ=255)                                                                                 ttCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcatt                                                                      >  1:1396860/1‑114 (MQ=255)                                                                                 ttCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCAtt                                             >  2:687463/1‑139 (MQ=255)                                                                                         gTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGAt                                                           <  2:970828/117‑1 (MQ=255)                                                                                         gTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGAt                                                           >  1:970828/1‑117 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             <  1:1555576/49‑1 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             >  2:1555576/1‑49 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         >  1:1305998/1‑69 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  2:1305998/69‑1 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGAc                                                                                                        <  2:1504922/70‑1 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGAc                                                                                                        >  1:1504922/1‑70 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATc                                   <  1:253236/139‑1 (MQ=255)                                                                                           tACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATc                                   >  2:1033139/1‑139 (MQ=255)                                                                                            aCGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                >  1:111358/1‑93 (MQ=255)                                                                                            aCGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                <  2:111358/93‑1 (MQ=255)                                                                                            aCGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCg                                  >  1:693589/1‑139 (MQ=255)                                                                                               ttATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:231407/77‑1 (MQ=255)                                                                                               ttATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:231407/1‑77 (MQ=255)                                                                                                  taAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                    >  2:436507/1‑99 (MQ=255)                                                                                                  taAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGttcattca                                                                    <  1:436507/99‑1 (MQ=255)                                                                                                    aaTTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:1439412/72‑1 (MQ=255)                                                                                                    aaTTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:1439412/1‑72 (MQ=255)                                                                                                       tgtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                               >  2:10121/1‑67 (MQ=255)                                                                                                       tgtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgcg                                                                                               <  1:10121/67‑1 (MQ=255)                                                                                                       tgtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgc                                                                                                <  2:274349/66‑1 (MQ=255)                                                                                                       tgtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAgc                                                                                                >  1:274349/1‑66 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    >  1:1740502/1‑45 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  2:1740502/45‑1 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:52489/71‑1 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          >  2:52489/1‑71 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      >  1:1502568/1‑139 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      >  1:1479504/1‑139 (MQ=255)                                                                                                        gtgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGGAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      <  1:1605748/139‑1 (MQ=255)                                                                                                         tgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                >  1:1217870/1‑80 (MQ=255)                                                                                                         tgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGc                                                                                <  2:1217870/80‑1 (MQ=255)                                                                                                         tgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccac                     >  2:774420/1‑139 (MQ=255)                                                                                                         tgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccac                     >  1:35291/1‑139 (MQ=255)                                                                                                         tgtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccac                     >  1:1521491/1‑139 (MQ=255)                                                                                                          gtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                    >  2:472611/1‑139 (MQ=255)                                                                                                          gtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                    <  1:774420/139‑1 (MQ=255)                                                                                                          gtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACcaca                    <  1:676120/139‑1 (MQ=255)                                                                                                          gtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCACATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      <  2:1033706/137‑1 (MQ=255)                                                                                                          gtgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCACATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      >  1:1033706/1‑137 (MQ=255)                                                                                                           tgA‑GAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAg                   <  1:870667/139‑1 (MQ=255)                                                                                                            ga‑gaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                               <  1:997571/78‑1 (MQ=255)                                                                                                            ga‑gaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGcc                                                                               >  2:997571/1‑78 (MQ=255)                                                                                                            ga‑gaAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGc                  >  1:511206/1‑139 (MQ=255)                                                                                                                  aTGCCGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCCCGACCATTACCGTCCATCGATTTTCGCACCAAAGCGGagc             >  1:786430/1‑139 (MQ=255)                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                    <  1:846529/60‑1 (MQ=255)                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                    >  2:846529/1‑60 (MQ=255)                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTc                                                    <  1:931942/92‑1 (MQ=255)                                                                                                                          ttCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTc                                                    >  2:931942/1‑92 (MQ=255)                                                                                                                           tCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGt    <  2:511206/139‑1 (MQ=255)                                                                                                                             cccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCg  >  2:1013605/1‑139 (MQ=255)                                                                                                                             cccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCg  >  1:1125893/1‑139 (MQ=255)                                                                                                                                    cGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:533943/1‑41 (MQ=255)                                                                                                                                    cGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:533943/41‑1 (MQ=255)                                                                                                                                        |                                                                                                                                GTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGA‑GAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352987‑3353248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑