Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,400,773	A→G	R105R (CGT→CGC)	mreB ←	cell wall structural complex MreBCD, actin‑like component MreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,400,773	0	A	G	100.0%	212.7 / NA	58	R105R (CGT→CGC)	mreB	cell wall structural complex MreBCD, actin‑like component MreB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/27);  total (31/27)

CAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCT  >  NC_000913/3400640‑3400906                                                                                                                                      |                                                                                                                                      cAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt                                                                                                                                  <  2:552641/139‑1 (MQ=255)   aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                                                                                                                                >  2:1059902/1‑139 (MQ=255)   aTTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCAGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATaa                                                                                                                                >  1:780134/1‑139 (MQ=255)     tGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAg                                                                                                                              <  1:1053332/139‑1 (MQ=255)        ggCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTg                                                                                                                           <  1:395149/139‑1 (MQ=255)         gCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt                                                                                                                          >  2:1608761/1‑139 (MQ=255)             ccATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCt                                                                                                                      <  1:1703222/139‑1 (MQ=255)             ccATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCt                                                                                                                      >  2:1703222/1‑139 (MQ=255)             ccATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCt                                                                                                                      <  1:1456562/139‑1 (MQ=255)                     tcttcAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACtt                                                                                                              <  2:72833/139‑1 (MQ=255)                             cAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                                      <  2:1711167/139‑1 (MQ=255)                                gAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGc                                                                                                                  >  1:557819/1‑124 (MQ=255)                                gAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGc                                                                                                                  <  2:557819/124‑1 (MQ=255)                                   gACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGc                                                                                                >  2:1728862/1‑139 (MQ=255)                                      ttCACGGGCTCCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg                                                                                             <  1:1059902/139‑1 (MQ=255)                                      ttCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                                      >  1:1279995/1‑130 (MQ=255)                                      ttCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                                      <  2:1279995/130‑1 (MQ=255)                                      ttCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg                                                                                             >  2:1578475/1‑139 (MQ=255)                                        cACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg                                                                                           >  2:1270243/1‑139 (MQ=255)                                                ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt                                                                                                                          <  2:396643/100‑1 (MQ=255)                                                ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGt                                                                                                                          >  1:396643/1‑100 (MQ=255)                                                       ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc                                                                            >  2:1665170/1‑139 (MQ=255)                                                        cTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGTTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACg                                                                           <  1:1270243/139‑1 (MQ=255)                                                         tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt                                                                                                                                  >  2:1335130/1‑83 (MQ=255)                                                         tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCAt                                                                                                                                  <  1:1335130/83‑1 (MQ=255)                                                         tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttttt                                                                                   <  2:30355/130‑1 (MQ=255)                                                         tGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttttt                                                                                   >  1:30355/1‑130 (MQ=255)                                                           cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCAc                                                                                                                <  1:399303/99‑1 (MQ=255)                                                           cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCAc                                                                                                                >  2:399303/1‑99 (MQ=255)                                                                aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTc                                                                   <  1:1578475/139‑1 (MQ=255)                                                                     cGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGa                                                              >  2:1368660/1‑139 (MQ=255)                                                                            cgcgGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc                                                       >  2:1157057/1‑139 (MQ=255)                                                                            cgcgGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc                                                       >  1:127085/1‑139 (MQ=255)                                                                            cgcgGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGcc                                                       >  2:429488/1‑139 (MQ=255)                                                                                         cTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGGACTTGTTTGATGAAGTGCTGGAGCATTTTTTTAGTCACGAAGAAGTCAGCGATAACGCCGTCTTACATTggg                                          <  2:1096243/139‑1 (MQ=255)                                                                                           gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                                      <  1:362329/77‑1 (MQ=255)                                                                                           gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTtgatg                                                                                                      >  2:362329/1‑77 (MQ=255)                                                                                           gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACTCCGTCtctgat                                              <  1:1043244/133‑6 (MQ=255)                                                                                           gggTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCAt                                              >  2:1043244/1‑133 (MQ=255)                                                                                                gcgcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATgg                                   >  2:741008/1‑139 (MQ=255)                                                                                                  gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGtt                                                                                                           >  2:1339981/1‑65 (MQ=255)                                                                                                  gcCAACCGGCACACAAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGtt                                                                                                           <  1:1339981/65‑1 (MQ=255)                                                                                                             cacaAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGcc                      <  1:1665170/139‑1 (MQ=255)                                                                                                             cacaAACCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGcc                      <  1:1157057/139‑1 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg                                                         <  1:299877/101‑1 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg                                                         >  2:299877/1‑101 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag                                <  1:1022438/126‑1 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag                                >  2:1238437/1‑126 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag                                >  2:1022438/1‑126 (MQ=255)                                                                                                                aaaCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGgcag                                <  1:1238437/126‑1 (MQ=255)                                                                                                                  aCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg                                                         <  1:851995/99‑1 (MQ=255)                                                                                                                  aCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACg                                                         >  2:851995/1‑99 (MQ=255)                                                                                                                  aCCAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCg                 >  2:496596/1‑139 (MQ=255)                                                                                                                    cAGAACGCGCGGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTa               <  1:612417/139‑1 (MQ=255)                                                                                                                            gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAg       >  2:264595/1‑139 (MQ=255)                                                                                                                            gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAg       >  2:1169818/1‑139 (MQ=255)                                                                                                                            gcgGGCTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAg       >  1:1015742/1‑139 (MQ=255)                                                                                                                                 cTTGGGCGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCt  >  2:1406185/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                      CAATTGCGGCAGCCATCGGTTCTTCAATCAGGAAGACTTCACGGGCACCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCACGAAGAAGTCGGCGATAACGCCGTCTTTCATTGGGCGAATGGCAGCAATATTGCCCGGCGTACGGCCCAGCATCT  >  NC_000913/3400640‑3400906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑