Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,440,950 G→A T27I (ACC→ATC)  rpoA ← RNA polymerase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,440,9500GA100.0% 366.5 / NA 114T27I (ACC→ATC) rpoARNA polymerase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (57/57);  total (57/57)

Only 100 of 114 total aligned reads displayed.

GCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGT  >  NC_000913/3440823‑3441083
                                                                                                                                 |                                                                                                                                     
gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcg                                                                                                                            >  2:288590/1‑139 (MQ=255)
gCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcg                                                                                                                            >  2:544262/1‑139 (MQ=255)
 cTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgt                                                                                                                           <  2:487282/139‑1 (MQ=255)
   gTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgc                                                                                                                         >  2:1619546/1‑139 (MQ=255)
    tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  1:4323/1‑139 (MQ=255)
    tACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  1:1442077/1‑139 (MQ=255)
        cATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGa                                                                                                                    <  1:1170214/139‑1 (MQ=255)
           gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        <  1:1170034/132‑1 (MQ=255)
           gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  2:1170034/1‑132 (MQ=255)
           gtagtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACt                                                                                                                 >  1:750371/1‑139 (MQ=255)
              gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  2:911651/1‑139 (MQ=255)
              gtaCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCAc                                                                                                              >  1:865796/1‑139 (MQ=255)
               taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        <  2:582262/128‑1 (MQ=255)
               taCACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  1:582262/1‑128 (MQ=255)
                 cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                           >  1:1151959/1‑139 (MQ=255)
                 cacCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTg                                                                                                           >  2:953266/1‑139 (MQ=255)
                   ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgt                                                                                                                           <  2:1283167/121‑1 (MQ=255)
                   ccATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:288590/139‑1 (MQ=255)
                    cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                                        >  1:209098/1‑139 (MQ=255)
                    cATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTc                                                                                                        >  2:1042281/1‑139 (MQ=255)
                     aTCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCg                                                                                                       >  2:730742/1‑139 (MQ=255)
                      tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        <  2:26614/121‑1 (MQ=255)
                      tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgtgcg                                                                                                                        >  1:26614/1‑121 (MQ=255)
                      tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:1000494/136‑1 (MQ=255)
                      tCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGa                                                                                                      >  2:1108977/1‑139 (MQ=255)
                         aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  2:967029/1‑139 (MQ=255)
                         aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  1:231938/1‑139 (MQ=255)
                         aTCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGatat                                                                                                   >  1:1570393/1‑139 (MQ=255)
                                ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                    <  2:1645759/131‑1 (MQ=255)
                                ccTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                    >  1:1645759/1‑131 (MQ=255)
                                 cTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                                           <  1:953266/139‑1 (MQ=255)
                                   cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  2:923499/139‑1 (MQ=255)
                                   cGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  1:967029/139‑1 (MQ=255)
                                    ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                                           >  2:584428/1‑136 (MQ=255)
                                    ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAgg                                                                                           <  1:584428/136‑1 (MQ=255)
                                    ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                        >  2:593141/1‑139 (MQ=255)
                                    ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                        >  2:1600943/1‑139 (MQ=255)
                                    ggTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcgc                                                                                        >  1:1291697/1‑139 (MQ=255)
                                       cACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGt                                                                                     <  1:364870/139‑1 (MQ=255)
                                          cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   <  1:420197/106‑1 (MQ=255)
                                          cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGaa                                                                                                                   >  2:420197/1‑106 (MQ=255)
                                          cgcgCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGtttt                                                                                  >  1:727052/1‑139 (MQ=255)
                                             gcAACCCGGTATCGATGAGAGCAGAATACGGCGCTGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGa                                                                               <  2:1151959/139‑1 (MQ=255)
                                                    ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                          <  1:890169/137‑1 (MQ=255)
                                                    ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAActc                                                                          >  2:890169/1‑137 (MQ=255)
                                                    ggCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTg                                                                        >  1:1290784/1‑139 (MQ=255)
                                                         cGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  2:1389745/139‑1 (MQ=255)
                                                           aTGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGa                                                                 >  2:1617807/1‑139 (MQ=255)
                                                                   aGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCa                                                         <  1:1617807/139‑1 (MQ=255)
                                                                          ggCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTc                                                  <  2:209098/139‑1 (MQ=255)
                                                                            cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                <  1:1619546/139‑1 (MQ=255)
                                                                            cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                >  2:201630/1‑139 (MQ=255)
                                                                            cgcAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                <  1:1600943/139‑1 (MQ=255)
                                                                               aGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                                             >  1:1513313/1‑139 (MQ=255)
                                                                               aGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTCCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCctctc                                             >  1:1284120/1‑139 (MQ=255)
                                                                                   cGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGgcg                                                                                         <  1:707419/91‑1 (MQ=255)
                                                                                   cGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                                         <  2:231938/139‑1 (MQ=255)
                                                                                   cGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTg                                         <  2:727052/139‑1 (MQ=255)
                                                                                      tACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGTACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                                      <  2:1513313/139‑1 (MQ=255)
                                                                                      tACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTa                                      <  2:1284120/139‑1 (MQ=255)
                                                                                       aCCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCttttgcac                                     <  1:1042281/139‑6 (MQ=255)
                                                                                       aCCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTAc                                     <  2:1442077/139‑1 (MQ=255)
                                                                                       aCCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTAc                                     <  1:361918/139‑1 (MQ=255)
                                                                                        cccAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACt                                    <  2:1291697/139‑1 (MQ=255)
                                                                                           agagTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                    <  1:146480/72‑1 (MQ=255)
                                                                                           agagTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGata                                                                                                    >  2:146480/1‑72 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  2:281879/103‑1 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  1:281879/1‑103 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  1:1562411/1‑103 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  1:1108415/127‑1 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  2:1108415/1‑127 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCt                               >  2:772453/1‑139 (MQ=255)
                                                                                             agTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTGGTACTAAGCtt                              >  1:834544/1‑139 (MQ=255)
                                                                                               tATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                <  1:1754732/120‑1 (MQ=255)
                                                                                               tATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                >  2:1754732/1‑120 (MQ=255)
                                                                                                  ggCCAAAGCCACGCTCTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTa                            >  1:1010093/1‑139 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCa                                                                                                               <  1:1710319/54‑1 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCa                                                                                                               >  2:1710319/1‑54 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:49129/1‑60 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:49129/60‑1 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  2:1282896/1‑98 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                          >  1:484835/1‑139 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACt                          <  2:865796/139‑1 (MQ=255)
                                                                                                  ggCCAAAGCCACG‑‑CTCTAAAGCCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  1:1282896/98‑1 (MQ=255)
                                                                                                   gCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           <  2:1738938/121‑1 (MQ=255)
                                                                                                   gCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCtt                                           >  1:1738938/1‑121 (MQ=255)
                                                                                                       aaGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgt                                                                                                                           >  2:526572/1‑37 (MQ=255)
                                                                                                       aaGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGgcgt                                                                                                                           <  1:526572/37‑1 (MQ=255)
                                                                                                       aaGCCACG‑‑CTCTAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCa                                                                                                               <  2:1543162/49‑1 (MQ=255)
                                                                                                                   ctAAAGGCTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTcga           <  2:750371/139‑1 (MQ=255)
                                                                                                                     aaaGGCTTAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCttagta         <  1:911651/139‑6 (MQ=255)
                                                                                                                          cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:911855/1‑38 (MQ=255)
                                                                                                                          cTCAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:911855/38‑1 (MQ=255)
                                                                                                                            cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         >  2:1295097/1‑36 (MQ=255)
                                                                                                                            cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCt                                                                                                         <  1:1295097/36‑1 (MQ=255)
                                                                                                                            cAAGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCct                                                >  2:797057/1‑93 (MQ=255)
                                                                                                                              aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   <  2:776516/72‑1 (MQ=255)
                                                                                                                              aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTcaca                                                                   >  1:776516/1‑72 (MQ=255)
                                                                                                                              aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCtta            >  2:738347/1‑125 (MQ=255)
                                                                                                                              aGGATCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCtta            <  1:738347/127‑3 (MQ=255)
                                                                                                                                 |                                                                                                                                     
GCTGTACTCATGTAGTACACCATCAATCTCAACCTCGGTCACCGCGCAACCCGGCATCGATGAGAGCAGAATACGGCGCAGTGCGTTACCCAGAGTATGGCCAAAGCCACG‑‑CTCTAAAGGCTCAAGGGTCACCTTGGCGTGCGTCGAACTCACTTGCTCGATATCAACCAGGCGCGGTTTTAGAAACTCTGTCACAGAACCCTGCATTGTGTCCTCTCTTTGGTACTAAGCTTTACTTGGAGTAAAGCTCGACGATCAGGT  >  NC_000913/3440823‑3441083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: