Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,589,820 T→C S70G (AGC→GGC)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,589,8200TC98.8% 310.8 / NA 86S70G (AGC→GGC) ugpEsn‑glycerol‑3‑phosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (47/38);  minor base A (0/1);  total (47/39)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAA  >  NC_000913/3589685‑3589955
                                                                                                                                       |                                                                                                                                       
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATGCGCCAGAACGGCGCGCAAtt                                                                                                                                      <  2:1221758/139‑1 (MQ=255)
agaagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:821041/139‑1 (MQ=255)
  aagaagaGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCg                                                                                                                                    >  2:303525/1‑139 (MQ=255)
            tACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTAcccc                                                                                                                          <  1:255307/139‑1 (MQ=255)
             aCGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                         >  2:1610574/1‑139 (MQ=255)
             aCGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                         <  2:977529/139‑1 (MQ=255)
             aCGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCg                                                                                                                         <  1:1515339/139‑1 (MQ=255)
              cGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                        >  1:1715771/1‑139 (MQ=255)
              cGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                        <  1:828626/139‑1 (MQ=255)
              cGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGt                                                                                                                        >  1:575646/1‑139 (MQ=255)
               gTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       >  1:105048/1‑139 (MQ=255)
               gTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       >  1:443700/1‑139 (MQ=255)
               gTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       <  2:126512/139‑1 (MQ=255)
                  gCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAc                                                                                                                    >  1:1193783/1‑139 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:817756/120‑1 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      >  1:817756/1‑120 (MQ=255)
                   cGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAcc                                                                                                                   >  2:1501736/1‑139 (MQ=255)
                    ggAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAccc                                                                                                                  <  1:303525/139‑1 (MQ=255)
                      aaaaCGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAg                                                                                                                <  2:575646/139‑1 (MQ=255)
                      aaaaCGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAg                                                                                                                <  2:1193783/139‑1 (MQ=255)
                                   aCAATGGCAAATGCCGAGAGCATCGAGACGGTAATTCTGCCGAGCGTAATGCTGAACGCCATAACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGt                                                                                                   >  1:177201/1‑139 (MQ=255)
                                    cAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGtt                                                                                                  >  2:18809/1‑139 (MQ=255)
                                       tGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTc                                                                                               >  2:1531093/1‑139 (MQ=255)
                                           aaaTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      >  1:879999/1‑96 (MQ=255)
                                           aaaTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:510668/96‑1 (MQ=255)
                                           aaaTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      >  1:510668/1‑96 (MQ=255)
                                           aaaTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  2:879999/96‑1 (MQ=255)
                                             aTGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCcagcag                                                                                         >  2:580400/1‑139 (MQ=255)
                                                ccGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtg                                                                                      <  2:766954/139‑1 (MQ=255)
                                                   agagCATCGAGACGGGAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGAtgtgt                                                                                   >  2:239210/1‑139 (MQ=255)
                                                      gCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGcc                                                                                <  1:1489543/139‑1 (MQ=255)
                                                        aTCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgg                                                                              >  1:1579466/1‑139 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      >  2:1761817/1‑82 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCAtt                                                                                                                                      <  1:1761817/82‑1 (MQ=255)
                                                         tCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCggg                                                                             >  2:1652209/1‑139 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgggg                                                                            <  2:105048/139‑1 (MQ=255)
                                                          cGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCgggg                                                                            >  1:991486/1‑139 (MQ=255)
                                                              aCGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         >  2:1291311/1‑106 (MQ=255)
                                                              aCGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGAtgttgt                                                                                                         <  1:1291311/106‑1 (MQ=255)
                                                                ggTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGc                                                                      >  1:49973/1‑139 (MQ=255)
                                                                        tGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCgg                                                              <  1:1652209/139‑1 (MQ=255)
                                                                         gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                             <  1:18809/139‑1 (MQ=255)
                                                                         gCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgc                                                             >  1:312728/1‑139 (MQ=255)
                                                                                      cTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                                <  1:1501736/139‑1 (MQ=255)
                                                                                      cTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACgg                                                <  2:1715771/139‑1 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGa                                                                           >  2:1459233/1‑109 (MQ=255)
                                                                                         aaCGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGa                                                                           <  1:1459233/109‑1 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAcccgga                                                                                                               >  2:762006/1‑68 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCAcccgga                                                                                                               <  1:762006/71‑4 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcgc                                                           <  1:1651280/123‑1 (MQ=255)
                                                                                           cGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGgcgc                                                           >  2:1651280/1‑123 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCg                                                               <  2:827395/116‑1 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCg                                                               >  1:827395/1‑116 (MQ=255)
                                                                                              cATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTa                                        >  1:1440024/1‑139 (MQ=255)
                                                                                                        cTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       >  2:944669/1‑50 (MQ=255)
                                                                                                        cTGTTAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGtt                                                                                                                       <  1:944669/50‑1 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGTAGATGTGTGCCGGGGATg                                                                         <  1:997304/93‑1 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATg                                                                         >  2:997304/1‑93 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGcc                                              <  1:235620/120‑1 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGcc                                              >  2:235620/1‑120 (MQ=255)
                                                                                                           ttAAGCAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTcgccg                           >  1:1291847/1‑139 (MQ=255)
                                                                                                                cAACATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACa                      <  2:1291847/139‑1 (MQ=255)
                                                                                                                 aaCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaa                     <  2:443700/139‑1 (MQ=255)
                                                                                                                  aCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCAt                                                                 >  1:962650/1‑94 (MQ=255)
                                                                                                                  aCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCAt                                                                 <  2:962650/94‑1 (MQ=255)
                                                                                                                  aCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaaa                    >  1:836516/1‑139 (MQ=255)
                                                                                                                  aCATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaaa                    >  1:394662/1‑139 (MQ=255)
                                                                                                                   cATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAAc                   >  2:997119/1‑139 (MQ=255)
                                                                                                                   cATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAAc                   >  2:36494/1‑139 (MQ=255)
                                                                                                                   cATCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGATTATCCAGCGTCGCCGCGACAAAc                   <  1:1433972/139‑1 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTTTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTGTCCAACGTCGCCGCGACAAACGc                 >  1:1144449/1‑139 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGc                 >  1:480917/1‑139 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGc                 >  1:1344778/1‑139 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGc                 >  1:827167/1‑139 (MQ=255)
                                                                                                                     tCCGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGc                 >  1:1220035/1‑139 (MQ=255)
                                                                                                                       cGCCAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCa               <  1:984357/139‑1 (MQ=255)
                                                                                                                         ccAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACg             >  2:1235883/1‑139 (MQ=255)
                                                                                                                          cAGAACGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGt            <  2:1579466/139‑1 (MQ=255)
                                                                                                                              aCGGCGCGCCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAg        <  2:480917/139‑1 (MQ=255)
                                                                                                                                 gcgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCgg     >  1:915296/1‑139 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTGACCCAGATGTTGTGGATGTTTTCcagcag                                                                                         >  2:1097274/1‑54 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCcagcag                                                                                         <  1:1097274/54‑1 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaa                     <  2:1430807/122‑1 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACaa                     >  1:1430807/1‑122 (MQ=255)
                                                                                                                                  cgcgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCggg    >  2:1118834/1‑139 (MQ=255)
                                                                                                                                    cgcCATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGaa  <  1:536279/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AGAAGAAGAGGTTACGTAGCGGAAAACGAAACCAGACAATGGCAAATGCCGAGAGCATCGAGACGGTAATTTTGCCGAGCGTAATGCTGAACGCCATCACAAAGCTGTTAAGCAACATCCGCCAGAACGGCGCGCTATTCGTGCCTACCCCGTTCACCCAGATGTTGTGGATGTTTTCCAGCAGATGTGTGCCGGGGATGAGCGTCATCGGCGCGGCATAGACGGCCTGTTTATCCAGCGTCGCCGCGACAAACGCCACGTACAGCGGGAA  >  NC_000913/3589685‑3589955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: