| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,773,602 | T→C | V441A (GTT→GCT) | mtlA → | mannitol‑specific PTS enzyme: IIA, IIB and IIC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,773,602 | 0 | T | C | 100.0% | 170.6 / NA | 47 | V441A (GTT→GCT) | mtlA | mannitol‑specific PTS enzyme: IIA, IIB and IIC components |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/24); total (23/24) | |||||||||||
TTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT > NC_000913/3773470‑3773736 | ttCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCa > 2:1225072/1‑139 (MQ=255)ttCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCa < 1:857698/139‑1 (MQ=255) gTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGAAGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGcaca < 1:1225072/139‑1 (MQ=255) aGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGCCCGAACGCGCTATGCGCCAGGCTCCGc < 1:1767732/129‑1 (MQ=255) aGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGc > 2:1767732/1‑129 (MQ=255) aTTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATAttt < 2:838878/139‑1 (MQ=255) tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATA‑TTCGCTGACCAACTTCCTc > 2:891382/1‑139 (MQ=255) tCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCg > 1:1173880/1‑139 (MQ=255) tCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCg > 1:913230/1‑139 (MQ=255) aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg < 2:976024/139‑1 (MQ=255) aTTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg < 2:501115/139‑1 (MQ=255) ttCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGc < 1:1216692/139‑1 (MQ=255) cTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCCCT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt < 2:590280/139‑1 (MQ=255) aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg < 1:1060991/127‑1 (MQ=255) aaCAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg > 2:1060991/1‑127 (MQ=255) aGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc > 1:2761/1‑139 (MQ=255) cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGcc > 2:389929/1‑139 (MQ=255) cgcgATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTg > 2:1510195/1‑139 (MQ=255) aTCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGAc < 1:389929/139‑1 (MQ=255) aacCTGCGGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGaa < 2:1772938/135‑1 (MQ=255) aacCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGaa > 1:1772938/1‑135 (MQ=255) aacCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTc < 2:1173880/139‑1 (MQ=255) gccgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGtt > 1:32720/1‑139 (MQ=255) cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc < 1:959208/104‑1 (MQ=255) cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc > 2:959208/1‑104 (MQ=255) cgccAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTtgc > 1:306980/1‑139 (MQ=255) aTGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCa > 2:888267/1‑139 (MQ=255) cGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg < 1:221796/49‑1 (MQ=255) cGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCg > 2:221796/1‑49 (MQ=255) gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg < 2:913230/139‑1 (MQ=255) gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg > 1:149789/1‑139 (MQ=255) gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg > 2:470352/1‑139 (MQ=255) gTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACgg > 1:949824/1‑139 (MQ=255) cacCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATA‑TTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga < 1:891382/139‑1 (MQ=255) acCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga < 1:554/139‑1 (MQ=255) cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga < 2:306980/139‑1 (MQ=255) tgacctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGaaa > 2:960062/1‑139 (MQ=255) gaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga > 2:387703/1‑129 (MQ=255) gaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAaga < 1:387703/129‑1 (MQ=255) ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg < 1:1318578/66‑1 (MQ=255) ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCg > 2:1318578/1‑66 (MQ=255) ccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGa < 2:149789/139‑1 (MQ=255) gAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACa < 1:333228/139‑1 (MQ=255) aCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGc > 2:1003223/1‑139 (MQ=255) aTGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga > 2:74346/1‑118 (MQ=255) aTGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga < 1:74346/118‑1 (MQ=255) cAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCt < 2:949824/139‑1 (MQ=255) | TTCTGCGTAAGAAAATTCAGGATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACT‑‑CACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGACAGCT > NC_000913/3773470‑3773736 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |