| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,815,863 | C→T | pseudogene (19/48 nt) | rph ← | ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,815,863 | 0 | C | T | 97.8% | 137.5 / NA | 45 | pseudogene (19/48 nt) | rph | ribonuclease PH (defective),enzyme, Degradation of RNA, RNase PH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (22/22); minor base G (1/0); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGG > NC_000913/3815750‑3815975 | ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCggg < 2:875257/139‑1 (MQ=255) atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCca < 1:26503/139‑1 (MQ=255) atGGTTTCATGCCTTCGCCCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCca < 2:1207939/139‑1 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAAt > 2:836322/1‑103 (MQ=255) aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAAt < 1:836322/103‑1 (MQ=255) gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCACCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATGCCCCTCGGGCAAGAGCCAACAAGATGAg < 1:1057397/139‑1 (MQ=255) aCTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa > 2:1417654/1‑139 (MQ=255) ttttCTACAGACAAAAAAATAGGCGCCTCATCGGTCGCCTTAAAATTCAGTTTGCCGGCGCCGCCTTTTGCGTCGTTACAATGATTTCGATTCCCCTCGGGCCAGGCCCAACAAGATGAGTAGCTCTTCATGGGTGAAc > 2:1197715/1‑139 (MQ=255) ttttCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc < 1:1363885/101‑1 (MQ=255) ttttCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc > 2:1363885/1‑101 (MQ=255) tACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg > 2:1352970/1‑139 (MQ=255) cAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc < 2:310864/94‑1 (MQ=255) cAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGc > 1:310864/1‑94 (MQ=255) aaaaaaa‑aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg > 1:1231125/1‑110 (MQ=255) aaaaaaa‑aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAg < 2:1231125/110‑1 (MQ=255) aaaaaaa‑aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg > 2:1364527/1‑139 (MQ=255) aa‑aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc < 1:1352970/139‑1 (MQ=255) ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac > 2:176806/1‑91 (MQ=255) ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaac < 1:176806/91‑1 (MQ=255) ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc < 2:1729/139‑1 (MQ=255) ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc > 2:145362/1‑139 (MQ=255) cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 1:697776/74‑1 (MQ=255) cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt > 2:697776/1‑74 (MQ=255) cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 1:411206/139‑1 (MQ=255) atcaGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc > 1:859344/1‑139 (MQ=255) aGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt > 2:416962/1‑65 (MQ=255) aGTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCt < 1:416962/65‑1 (MQ=255) gTCGCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatg > 2:1394652/1‑139 (MQ=255) gCCTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg > 1:25435/1‑139 (MQ=255) ccTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg > 1:233095/1‑125 (MQ=255) ccTTAAAAATCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATGCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg < 2:233095/125‑1 (MQ=255) aaaaaTCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt < 1:1291045/51‑1 (MQ=255) aaaaaTCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGgattcgatt > 2:1291045/1‑51 (MQ=255) aTCAGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCt < 2:859344/139‑1 (MQ=255) aGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg > 2:1529858/1‑139 (MQ=255) aGTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg > 2:390646/1‑139 (MQ=255) gTTTGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg < 2:1742390/139‑1 (MQ=255) tttGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCag < 2:1312089/55‑1 (MQ=255) tttGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCag > 1:1312089/1‑55 (MQ=255) tGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGGGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt > 2:817798/1‑109 (MQ=255) tGCCGGCGCCGCCTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGGGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt < 1:817798/109‑1 (MQ=255) ccgccTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa < 2:1444118/51‑1 (MQ=255) ccgccTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa > 1:1444118/1‑51 (MQ=255) ccTTCTGCGTCGTTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc < 1:1519030/84‑1 (MQ=255) ccTTCTGCGTCGGTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc > 2:1519030/1‑84 (MQ=255) | GGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAA‑AGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGG > NC_000913/3815750‑3815975 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |