Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,862,497 C→T pseudogene (1107/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,862,4970CT100.0% 316.0 / NA 96pseudogene (1107/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS,enzyme, Transport of small molecules: Carbohydrates, organic acids, alcohols, PTS system, arbutin‑like IIB component, PTS system, arbutin‑like IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/51);  total (45/51)

ACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTT  >  NC_000913/3862364‑3862630
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acaTGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAATCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTc                                                                                                                                  <  2:39644/139‑1 (MQ=255)
    gTTGAACTGCAGAATCAGTGTGCGCAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATc                                                                                                                              <  2:1004492/139‑1 (MQ=255)
       gAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGa                                                                                                                           >  1:1422530/1‑139 (MQ=255)
            gCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTcc                                                                                                                      >  1:799630/1‑139 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  2:700507/139‑1 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:6995/1‑139 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:661889/1‑139 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:642073/1‑139 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  2:525817/139‑1 (MQ=255)
             cAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  1:661889/139‑1 (MQ=255)
              aGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  2:297256/123‑1 (MQ=255)
              aGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  1:297256/1‑123 (MQ=255)
              aGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAAt                                                                                                                               >  2:979577/1‑128 (MQ=255)
              aGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAAt                                                                                                                               <  1:979577/128‑1 (MQ=255)
              aGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGc                                                                                                                    >  2:297733/1‑139 (MQ=255)
               gAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGcc                                                                                                                   <  1:405937/139‑1 (MQ=255)
                  tCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGccatg                                                                                                                <  1:1373078/139‑4 (MQ=255)
                    aGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGtt                                                                                                             <  2:1061495/139‑1 (MQ=255)
                      tgtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                           <  2:1426781‑M1/139‑3 (MQ=255)
                      tgtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                           <  2:1422530‑M1/139‑3 (MQ=255)
                       gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  1:1250467/1‑114 (MQ=255)
                       gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  2:1250467/114‑1 (MQ=255)
                       gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  2:368064/129‑1 (MQ=255)
                       gtgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  1:368064/1‑129 (MQ=255)
                        tgCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcacc                                                                                                         >  2:384060‑M1/1‑135 (MQ=255)
                          cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  1:1695616/111‑1 (MQ=255)
                          cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  2:1695616/1‑111 (MQ=255)
                          cGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccac                                                                                                       >  1:486256‑M1/1‑133 (MQ=255)
                           ggAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccaca                                                                                                      <  2:1331146‑M1/139‑8 (MQ=255)
                                aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccaca                                                                                                      <  2:180759‑M1/134‑8 (MQ=255)
                                aCCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccaca                                                                                                      >  1:180759‑M1/1‑127 (MQ=255)
                                    cGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagaga                                                                                             <  1:483256‑M1/139‑17 (MQ=255)
                                          aCAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacatt                                                                                       <  1:1132681‑M1/139‑23 (MQ=255)
                                              cagGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    >  2:884710/1‑91 (MQ=255)
                                              cagGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAg                                                                                                                                    <  1:884710/91‑1 (MQ=255)
                                                 ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcg                                                                                >  1:762462‑M1/1‑110 (MQ=255)
                                                 ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagaga                                                                                             <  1:463269‑M1/126‑17 (MQ=255)
                                                 ggTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagaga                                                                                             >  2:463269‑M1/1‑110 (MQ=255)
                                                   tAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgac                                                                              >  1:911737‑M1/1‑108 (MQ=255)
                                                      aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                 >  2:395032‑M1/1‑105 (MQ=255)
                                                      aGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                 <  1:395032‑M1/117‑13 (MQ=255)
                                                           aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                           <  2:1195140‑M1/102‑3 (MQ=255)
                                                           aaCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTca                                                                                                           >  1:1195140‑M1/1‑100 (MQ=255)
                                                            aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagag                                                                                              <  2:437234‑M1/114‑16 (MQ=255)
                                                            aCCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagag                                                                                              >  1:437234‑M1/1‑99 (MQ=255)
                                                             cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattac                                                                                     <  1:567244‑M1/122‑25 (MQ=255)
                                                             cccAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattac                                                                                     >  2:567244‑M1/1‑98 (MQ=255)
                                                                aaTGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAAt                                                                                                                               >  2:1634795/1‑78 (MQ=255)
                                                                aaTGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAAt                                                                                                                               <  1:1634795/78‑1 (MQ=255)
                                                                aaTGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcca                                                                 >  2:305863‑M1/1‑95 (MQ=255)
                                                                aaTGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgcca                                                                 >  1:1686538‑M1/1‑95 (MQ=255)
                                                                  tGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                               <  2:911737‑M1/139‑47 (MQ=255)
                                                                     cGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacg                                                            <  2:486256‑M1/139‑50 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAGCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                          >  1:1154991‑M1/1‑88 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                          <  2:1154991‑M1/139‑52 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                   >  1:1794218‑M1/1‑88 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacacca                                                                                                   <  2:1794218‑M1/98‑11 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          >  2:721879‑M1/1‑88 (MQ=255)
                                                                       aTTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          <  1:721879‑M1/91‑4 (MQ=255)
                                                                          tGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGTGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagcgatacattacggtcgacattgaggccgccagcacggcgtg                                                       >  2:1201127‑M1/1‑85 (MQ=255)
                                                                           gggTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                 <  2:968209‑M1/95‑13 (MQ=255)
                                                                           gggTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTAATCAGACCTCCGCCATGTTcaccacaccaaa                                                                                                 >  1:968209‑M1/1‑83 (MQ=255)
                                                                                 gCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgca                                                <  1:150580‑M1/139‑62 (MQ=255)
                                                                                  catcatATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaa                                               >  1:1719302‑M1/1‑77 (MQ=255)
                                                                                         tCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaa                                        <  2:1722691‑M1/139‑70 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAc                                                                                                                          <  1:440556/55‑1 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAc                                                                                                                          >  2:440556/1‑55 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     >  2:1260899/1‑60 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCg                                                                                                                     <  1:1260899/60‑1 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacgg                                     >  1:1243686‑M1/1‑67 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          <  1:1468804‑M1/70‑4 (MQ=255)
                                                                                            gcgTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcac                                                                                                          >  2:1468804‑M1/1‑67 (MQ=255)
                                                                                               tGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAc                                                                                                                          >  1:1283230/1‑52 (MQ=255)
                                                                                               tGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGAc                                                                                                                          <  2:1283230/52‑1 (MQ=255)
                                                                                                  ttGCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaat                               >  1:952554‑M1/1‑61 (MQ=255)
                                                                                                    gCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacat                                                                                        >  2:309373‑M1/1‑59 (MQ=255)
                                                                                                    gCTGAACATCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacat                                                                                        <  1:309373‑M1/80‑22 (MQ=255)
                                                                                                            tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                              <  2:662609/63‑1 (MQ=255)
                                                                                                            tCGGGATCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTT‑‑‑CACCACACCaaa                                                                                              >  1:662609/1‑63 (MQ=255)
                                                                                                                  tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaat               >  2:39479‑M1/1‑45 (MQ=255)
                                                                                                                  tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                               <  2:1608419‑M1/91‑47 (MQ=255)
                                                                                                                  tCCAGTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                               >  1:1608419‑M1/1‑45 (MQ=255)
                                                                                                                      gTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGttcacc                                                                                                         >  1:482616/1‑41 (MQ=255)
                                                                                                                      gTTTTGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGttcacc                                                                                                         <  2:482616/45‑5 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag          <  1:305863‑M1/139‑100 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag          <  1:297733‑M1/139‑100 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag          <  1:642073‑M1/139‑100 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccag          <  1:331635‑M1/139‑100 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                               >  1:117860‑M1/1‑40 (MQ=255)
                                                                                                                       ttttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagc                                                               <  2:117860‑M1/86‑47 (MQ=255)
                                                                                                                        tttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  <  2:475914‑M1/66‑28 (MQ=255)
                                                                                                                        tttGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggt                                                                                  >  1:475914‑M1/1‑39 (MQ=255)
                                                                                                                          tGCGGTAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcgg       <  1:6995‑M1/139‑103 (MQ=255)
                                                                                                                               tAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggcgtgtaccgcaaacagcaacggtgaaatgaacaggaaggtaaattccagcggttcgg  <  2:1719302‑M1/139‑108 (MQ=255)
                                                                                                                               tAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                          >  1:773366‑M1/1‑32 (MQ=255)
                                                                                                                               tAAAACTTAGTCAATCAGACCTCCGCCATGTTcaccacaccaaagagatacattacggtcgacattgaggccgccagcacggc                                                          <  2:773366‑M1/83‑52 (MQ=255)
                                                                                                                                     |                                                                                                                                     
ACATGTTGAACTGCAGAATCAGTGTGCGGAAAACCACGAAGTACAGCAGGGTAAAGCACAACCCAATGGCGATTTGGGTCAGCATCATATCCGCGTGGTTGCTGAACATCGGGATCCAGTTTTGCGGTAAAACCTAGTCAATCAGACCTCCGCCCATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGCGGTT  >  NC_000913/3862364‑3862630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: