Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,939,219 G→A *331* (TAG→TAA
S465F (TCT→TTT) 
rbsR →
hsrA ←
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,939,2190GA100.0% 430.5 / NA 131*331* (TAG→TAA
S465F (TCT→TTT) 
rbsR
hsrA
transcriptional repressor of ribose metabolism
putative multidrug or homocysteine efflux system
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (61/70);  total (61/70)

Only 100 of 131 total aligned reads displayed.

ACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTT  >  NC_000913/3939088‑3939351
                                                                                                                                   |                                                                                                                                    
aCTATCCACCAACCGAAAGATGAACTGGGTGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                               <  1:1158928/139‑1 (MQ=255)
aCTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                               <  2:573167/139‑1 (MQ=255)
 cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                              <  2:1788366/139‑1 (MQ=255)
 cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                              <  1:396614/139‑1 (MQ=255)
 cTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                              <  2:3982/139‑1 (MQ=255)
  taaccaccaACCTGAAGATGAACTGGGGGAGCTGGCGATTGATGTACGCATCCATCGGATAACCCAGCCTACCCTTCAGCAACAACGAGTACAACTTACTCCGATTCTGGTGGAACGCGGTTCGGCGTAACTTTACACt                                                                                                                             <  1:1240625/136‑1 (MQ=255)
  tATCCACCCACCGAAAGATGACCGGGGGGAGCTGGCGATTGATGTACTCCTCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                             <  1:581749/139‑1 (MQ=255)
  tATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                             >  1:1150827/1‑139 (MQ=255)
  tATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                             <  2:1031938/139‑1 (MQ=255)
   aTCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTg                                                                                                                            >  2:432716/1‑139 (MQ=255)
    tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                                  <  2:189027/132‑1 (MQ=255)
    tCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGCTCGGCTTAAATTTACGCTGt                                                                                                                           >  1:1502750/1‑139 (MQ=255)
          aaCCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTg                                                                                                                     >  2:653118/1‑139 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   <  2:562774/139‑1 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   >  1:1179384/1‑139 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   >  1:808100/1‑139 (MQ=255)
            ccGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   >  1:717412/1‑139 (MQ=255)
                aaGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCaaa                                                                                                               <  2:1544618/139‑1 (MQ=255)
                      aaCTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAatta                                                                                                            >  2:1146610/1‑136 (MQ=255)
                                      gATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                         <  1:1343135/139‑1 (MQ=255)
                                              tACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATg                                                                                 >  1:544454/1‑139 (MQ=255)
                                                  catccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   >  1:808183/1‑101 (MQ=255)
                                                   atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                              <  1:122407/89‑1 (MQ=255)
                                                   atccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGc                                                                                                                              >  2:122407/1‑89 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                               >  1:500233/1‑86 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                               <  2:500233/86‑1 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                >  1:1273392/1‑117 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATTAACATtg                                                                          >  1:108594/1‑139 (MQ=255)
                                                     ccatcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          >  1:1727935/1‑139 (MQ=255)
                                                       atcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                >  1:194719/1‑115 (MQ=255)
                                                        tcGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtgctg                                                                       <  2:1179384/139‑1 (MQ=255)
                                                          ggATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTgctgct                                                                     <  2:387466/139‑1 (MQ=255)
                                                           gATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGctgctg                                                                    >  2:1703986/1‑139 (MQ=255)
                                                            aTAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGa                                                                   <  1:871378/139‑1 (MQ=255)
                                                             tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACg                                                                                                                               <  2:1446534/78‑1 (MQ=255)
                                                             tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          <  1:1150782/131‑1 (MQ=255)
                                                             tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          >  2:1150782/1‑131 (MQ=255)
                                                             tAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGaa                                                                  <  2:544454/139‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCgg                                                                                                  <  2:1641585/105‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  2:1353486/139‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  2:717412/139‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  1:432716/139‑1 (MQ=255)
                                                               aCCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  1:653118/139‑1 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                                  <  2:1288495/72‑1 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAAttt                                                                                                                                  >  1:1288495/1‑72 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                       >  2:877547/1‑115 (MQ=255)
                                                                cccAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                       <  1:877547/115‑1 (MQ=255)
                                                                  cAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAAc                                                             <  2:808100/139‑1 (MQ=255)
                                                                    gCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAg                                                           <  2:1727935/139‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTAcc                                                                                                       <  2:125509/94‑1 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                         >  2:47889/1‑108 (MQ=255)
                                                                     ccGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                         <  1:47889/108‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATg                                                                                 >  1:1406667/1‑111 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          <  2:1123619/118‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          <  2:757011/118‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          >  1:1123619/1‑118 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATtg                                                                          >  1:757011/1‑118 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  2:508606/128‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                >  1:508606/1‑128 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                <  2:653631/128‑1 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGc                                                                >  1:653631/1‑128 (MQ=255)
                                                                          ccTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGtaataa                                                     >  1:669661/1‑139 (MQ=255)
                                                                                  aacaacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCAtt                                             <  2:108594/139‑1 (MQ=255)
                                                                                    caacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgt                                           <  1:1703986/139‑1 (MQ=255)
                                                                                     aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                            >  2:277301/1‑105 (MQ=255)
                                                                                     aacGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACAt                                                                            <  1:277301/105‑1 (MQ=255)
                                                                                      acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                >  2:77307/1‑84 (MQ=255)
                                                                                      acGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                <  1:77307/84‑1 (MQ=255)
                                                                                         aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                             <  2:131751/52‑1 (MQ=255)
                                                                                         aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCt                                                                                                                             >  1:131751/1‑52 (MQ=255)
                                                                                         aTTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATaa                                      <  2:1318260/139‑1 (MQ=255)
                                                                                           tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAg                                                                                        <  2:1047032/87‑1 (MQ=255)
                                                                                           tACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTACTGCCATCGGTTGTTTTCAg                                                                                        >  1:1047032/1‑87 (MQ=255)
                                                                                            aCAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                   <  2:669661/139‑1 (MQ=255)
                                                                                              aaCTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGta                                 <  1:394463/139‑1 (MQ=255)
                                                                                                  tACTCCGATTCTGATGGATCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGt                             >  2:136590/1‑139 (MQ=255)
                                                                                                  tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGa                                                                                                                    <  1:1113883/52‑1 (MQ=255)
                                                                                                  tACTCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGgtt                                                                                                >  2:1172348/1‑72 (MQ=255)
                                                                                                     tCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   <  2:553281/50‑1 (MQ=255)
                                                                                                     tCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGAt                                                                                                                   >  1:553281/1‑50 (MQ=255)
                                                                                                     tCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                       <  2:888883/78‑1 (MQ=255)
                                                                                                     tCCGATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGa                                                                                       >  1:888883/1‑78 (MQ=255)
                                                                                                        gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgt                                           >  2:290163/1‑119 (MQ=255)
                                                                                                        gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATtgt                                           <  1:290163/119‑1 (MQ=255)
                                                                                                        gATTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAAtt                       >  1:1403030/1‑139 (MQ=255)
                                                                                                         aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATg                                                                                 <  1:1441051/80‑1 (MQ=255)
                                                                                                         aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATg                                                                                 >  2:1441051/1‑80 (MQ=255)
                                                                                                         aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                         >  2:1607890/1‑120 (MQ=255)
                                                                                                         aTTCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGa                                         <  1:1607890/120‑1 (MQ=255)
                                                                                                          ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                   >  2:416117/1‑125 (MQ=255)
                                                                                                          ttCTGATGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACg                                   <  1:416117/125‑1 (MQ=255)
                                                                                                               aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTc                   >  1:796645/1‑136 (MQ=255)
                                                                                                               aTGGAACGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTc                   <  2:796645/136‑1 (MQ=255)
                                                                                                                    aCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATc                                                                                                                  >  1:1653307/1‑36 (MQ=255)
                                                                                                                    aCGCGGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCg           <  1:335608/139‑1 (MQ=255)
                                                                                                                     cgcgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGt          >  1:1518930/1‑139 (MQ=255)
                                                                                                                       cgGTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCa                                                                                         >  2:295492/1‑58 (MQ=255)
                                                                                                                         gTTCGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTtgtg      <  2:82915/139‑1 (MQ=255)
                                                                                                                            cGGCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCt   <  2:1403030/139‑1 (MQ=255)
                                                                                                                             ggCTTAAATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCtt  >  2:1610204/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
ACTATCCACCAACCGAAAGATGAACTGGGGGAGCTGGCGATTGATGTACTCATCCATCGGATAACCCAGCCGACCCTTCAGCAACAACGATTACAACTTACTCCGATTCTGATGGAACGCGGTTCGGCTTAGATTTACGCTGTCTTTTGATCAAATTATTACCATCGGTTGTTTTCAGAAGCATGAACATTGCTGCTGAAGCAACAGTAATAATGCCCATTGTGATAAACGTATAGTGGAATTGTTCGACAGTCGTTGTGCCTT  >  NC_000913/3939088‑3939351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: