Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,352,880 G→A *99* (TAG→TAA ghoS → antitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,352,8800GA98.5% 211.8 / NA 67*99* (TAG→TAAghoSantitoxin of GhoTS toxin‑antitoxin pair, endonuclease for ghoT mRNA
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (26/40);  total (26/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTA  >  NC_000913/4352746‑4353009
                                                                                                                                      |                                                                                                                                 
ttCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCag                                                                                                                               <  1:1053782/139‑1 (MQ=255)
ttCGCAATAACGACATAGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGAATTACTGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCag                                                                                                                               <  1:75527/139‑1 (MQ=255)
 tCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                              <  1:1045316/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  2:1161790/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:640629/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  2:708296/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:474151/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:1627/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:438636/139‑1 (MQ=255)
  cGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTGTACAAGTAACCagga                                                                                                                             <  1:698045/139‑1 (MQ=255)
         aCGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATaa                                                                                                                      >  2:1714986/1‑139 (MQ=255)
              aTCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  2:590958/127‑1 (MQ=255)
              aTCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  1:590958/1‑127 (MQ=255)
              aTCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAagaaga                                                                                                                 >  1:279672/1‑139 (MQ=255)
                  ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCag                                                                                                                               <  1:124050/121‑1 (MQ=255)
                  ttAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCag                                                                                                                               >  2:124050/1‑121 (MQ=255)
                    aGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                              >  1:624968/1‑120 (MQ=255)
                    aGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagg                                                                                                                              <  2:624968/120‑1 (MQ=255)
                        gCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                       <  1:143612/139‑1 (MQ=255)
                         cAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAAt                                                                                                      >  1:1752306/1‑139 (MQ=255)
                          aaGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:1391962/115‑1 (MQ=255)
                          aaGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  2:1391962/1‑115 (MQ=255)
                             tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                  <  1:611871/139‑1 (MQ=255)
                             tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                  <  2:592937/139‑1 (MQ=255)
                             tGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                  <  1:1714986/139‑1 (MQ=255)
                               aaaTCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACt                                                                                                >  1:1371648/1‑139 (MQ=255)
                                                  gCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAAtt                                                                             >  1:494334/1‑139 (MQ=255)
                                                  gCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACCATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAAtt                                                                             >  1:1301041/1‑139 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:904728/81‑1 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  2:904728/1‑81 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                       <  2:1492934/103‑1 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                       >  1:1492934/1‑103 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   <  2:1371648/139‑1 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   <  1:1012957/139‑1 (MQ=255)
                                                            ttAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   <  2:279672/139‑1 (MQ=255)
                                                                  ttACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  1:482570/1‑75 (MQ=255)
                                                                  ttACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  2:482570/75‑1 (MQ=255)
                                                                  ttACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgtg                                                             <  2:380780/139‑1 (MQ=255)
                                                                    aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   <  1:17843/131‑1 (MQ=255)
                                                                    aCCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   >  2:17843/1‑131 (MQ=255)
                                                                        gATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACa                                                       <  2:693041/139‑1 (MQ=255)
                                                                          tatTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGTACTATTCTCTAAAATATTAATTTTTTa                                                                        <  1:1072090/120‑1 (MQ=255)
                                                                          tatTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGACACTATTCTCTAAAATATTAATTTTTTa                                                                        >  2:1072090/1‑120 (MQ=255)
                                                                              gATTTTGACCTCAACATTATGACAGTTGATGACCATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCt                                                 <  2:1301041/139‑1 (MQ=255)
                                                                                ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  2:1455550/1‑61 (MQ=255)
                                                                                ttttGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:1455550/61‑1 (MQ=255)
                                                                                   tGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             <  1:284788/58‑1 (MQ=255)
                                                                                   tGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCagga                                                                                                                             >  2:284788/1‑58 (MQ=255)
                                                                                   tGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTc                                            <  2:494334/139‑1 (MQ=255)
                                                                                                 tGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                  <  2:138007/71‑1 (MQ=255)
                                                                                                 tGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCa                                                                                                  >  1:138007/1‑71 (MQ=255)
                                                                                                 tGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTAt                              >  2:1255262/1‑139 (MQ=255)
                                                                                                      gTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                       <  2:643405/61‑1 (MQ=255)
                                                                                                      gTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATaaaaaa                                                                                                       >  1:643405/1‑61 (MQ=255)
                                                                                                      gTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                              >  2:95616/1‑102 (MQ=255)
                                                                                                      gTTGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGgt                                                              <  1:95616/102‑1 (MQ=255)
                                                                                                       ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   <  2:505563/96‑1 (MQ=255)
                                                                                                       ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGa                                                                   >  1:505563/1‑96 (MQ=255)
                                                                                                       ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                        <  2:149257/139‑1 (MQ=255)
                                                                                                       ttGATGACTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTcaca                        >  1:149257/1‑139 (MQ=255)
                                                                                                             aCTATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGaa                  <  2:1752306/139‑1 (MQ=255)
                                                                                                               tATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAtt                                                                                             >  2:1366456/1‑62 (MQ=255)
                                                                                                               tATTTCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTAtt                                                                                             <  1:1366456/62‑1 (MQ=255)
                                                                                                                   tCCGTCAGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAGAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAAcaca            <  1:1255262/139‑1 (MQ=255)
                                                                                                                         aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                  >  2:431118/1‑79 (MQ=255)
                                                                                                                         aGTTTTACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGAt                                                                  <  1:431118/79‑1 (MQ=255)
                                                                                                                             ttACAAGTAACCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTa  >  2:1423903/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                 
TTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATTGATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAGCCAGGAAGGATAAGAAGAACATAAAAAATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACATGAGAAAACACATATTCGTTTA  >  NC_000913/4352746‑4353009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: