Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,424,386 G→A *229* (TAG→TAA ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,424,3860GA100.0% 227.7 / NA 70*229* (TAG→TAAulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/34);  total (36/34)

AGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGT  >  NC_000913/4424260‑4424514
                                                                                                                              |                                                                                                                                
agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcg                                                                                                                            >  1:970446/1‑133 (MQ=255)
agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACAcg                                                                                                                            <  2:970446/133‑1 (MQ=255)
  aagTGGCGAAAATGGGGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCgg                                                                                                                    >  1:1198784/1‑139 (MQ=255)
  aagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCgg                                                                                                                    >  2:647189/1‑139 (MQ=255)
          aaaaTGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcc                                                                                                            <  2:911645/139‑1 (MQ=255)
                    ggATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGga                                                                                                  <  1:352760/139‑1 (MQ=255)
                     gATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGCAAATTTCCTTCCTCGGgag                                                                                                 <  2:1198784/139‑1 (MQ=255)
                                           caACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGc                                                                           <  1:647189/139‑1 (MQ=255)
                                                 aaTCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGtttt                                                                     >  2:1743546/1‑139 (MQ=255)
                                                      caTCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGga                                                                                                  <  2:1019807/105‑1 (MQ=255)
                                                      caTCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGga                                                                                                  >  1:1019807/1‑105 (MQ=255)
                                                        tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGga                                                                                                  >  2:1029499/1‑103 (MQ=255)
                                                        tCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaa                                                              >  1:1278162/1‑139 (MQ=255)
                                                        tCGACAGCTTCCTGATGAATAAACACTTCATACGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGCAATTTCCTTCCTCGGga                                                                                                  <  1:1029499/103‑1 (MQ=255)
                                                           aCAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCaaaaaaaa                                                           <  2:133847/139‑1 (MQ=255)
                                                                 tCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGccccc                                                     >  1:750384/1‑139 (MQ=255)
                                                                         aaTAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAgg                                             >  1:647458/1‑139 (MQ=255)
                                                                           tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                           >  2:798481/1‑75 (MQ=255)
                                                                           tAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                           <  1:798481/75‑1 (MQ=255)
                                                                            aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                          <  2:750384/139‑1 (MQ=255)
                                                                            aaaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                          <  1:1014422/139‑1 (MQ=255)
                                                                             aaCACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgc                                         <  2:1278162/139‑1 (MQ=255)
                                                                                acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       <  2:1164391/74‑1 (MQ=255)
                                                                                acTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       >  1:1164391/1‑74 (MQ=255)
                                                                                  ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       >  2:789633/1‑72 (MQ=255)
                                                                                  ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       <  1:789633/72‑1 (MQ=255)
                                                                                  ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCa                                                                  >  1:1273021/1‑109 (MQ=255)
                                                                                  ttCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCa                                                                  <  2:1273021/109‑1 (MQ=255)
                                                                                   tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                           >  1:1183909/1‑67 (MQ=255)
                                                                                   tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATttcct                                                                                                           <  2:1183909/67‑1 (MQ=255)
                                                                                   tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   >  2:1180047/1‑139 (MQ=255)
                                                                                   tCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   <  1:1304716/139‑1 (MQ=255)
                                                                                    cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACgcgc                                                                                                                         >  2:1604453/1‑52 (MQ=255)
                                                                                    cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACgcgc                                                                                                                         <  1:1604453/52‑1 (MQ=255)
                                                                                    cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAGAAAAGCCCCCTGCGAAGGGGgcaaagcaa                                  >  2:33745/1‑139 (MQ=255)
                                                                                    cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                          >  1:540785/1‑131 (MQ=255)
                                                                                    cATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAggggg                                          <  2:540785/131‑1 (MQ=255)
                                                                                        cGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTa                              >  2:1604509/1‑139 (MQ=255)
                                                                                          tAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATg                            >  1:977073/1‑139 (MQ=255)
                                                                                            aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                         >  1:1553577/1‑108 (MQ=255)
                                                                                            aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGc                                                         <  2:1553577/108‑1 (MQ=255)
                                                                                            aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   >  2:1006189/1‑130 (MQ=255)
                                                                                            aaCACGGTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   <  1:1006189/130‑1 (MQ=255)
                                                                                                 ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTc                                                                                                      <  2:973410/58‑1 (MQ=255)
                                                                                                 ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTc                                                                                                      >  1:973410/1‑58 (MQ=255)
                                                                                                 ggTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGt                     >  1:1557228/1‑139 (MQ=255)
                                                                                                  gTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       <  2:115178/56‑1 (MQ=255)
                                                                                                  gTCCTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTtccttcct                                                                                                       >  1:115178/1‑56 (MQ=255)
                                                                                                     cTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                 <  2:1557228/139‑1 (MQ=255)
                                                                                                     cTAACGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                 <  1:1604509/139‑1 (MQ=255)
                                                                                                        aCGCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAAATATGGCAATGTTTCGTTg              >  2:809255/1‑139 (MQ=255)
                                                                                                          gCTTATTACGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt            <  1:809255/139‑1 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTGCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat         >  2:1173461/1‑139 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGccccc                                                     <  1:504780/95‑1 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGccccc                                                     >  2:504780/1‑95 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   <  1:808428/113‑1 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGgcaaagca                                   >  2:808428/1‑113 (MQ=255)
                                                                                                             tattaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTtat         >  1:1512781/1‑139 (MQ=255)
                                                                                                               ttaCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATAc       <  2:1596822/139‑1 (MQ=255)
                                                                                                                 aCGGGCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAGAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCt     <  1:33745/139‑1 (MQ=255)
                                                                                                                   gggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTgg   >  1:556105/1‑139 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                      >  2:1414550/1‑55 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCt                                                                                      <  1:1414550/55‑1 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                 >  1:1617876/1‑124 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCg                 <  2:1617876/124‑1 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt            <  1:1480753/129‑1 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGt            >  2:1480753/1‑129 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCt     <  2:585424/136‑1 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCt     >  1:585424/1‑136 (MQ=255)
                                                                                                                    ggCAGAAGTAAAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGt  >  1:455126/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                
AGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGAATAAACACTTCATGCGTAAACACGGTCCTAACGCTTATTACGGGCAGAAGTAGAACACGCGCTGCGGAAATTTCCTTCCTCGGGAGATAACTGGTCTAATTCCGCAGCCGTTTTTCAAAAAAAAGCCCCCTGCGAAGGGGGCAAAGCAAACTATGGCAATGTTTCGTTGGTTATACCTGGT  >  NC_000913/4424260‑4424514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: