Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,737	A→G	D169G (GAT→GGT)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,737	0	A	G	100.0%	149.8 / NA	42	D169G (GAT→GGT)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

GCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTT  >  NC_000913/385607‑385870                                                                                                                                   |                                                                                                                                      gCCGTACCGTTTATCTCCATCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGGGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                               <  1:1173690/139‑1 (MQ=255) gCCGTACCGTTTATCTCCACCACCCTCTACAGCCTGCTGGCGGCACTGCAACACTGGGGTATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCACCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATGGATg                                                                                                                               <  1:786893/139‑1 (MQ=255)          tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                      >  1:910135/1‑139 (MQ=255)          tttATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATg                                                                                                                      >  1:389366/1‑139 (MQ=255)                       cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcggc                                                                                                         >  2:1273488/1‑139 (MQ=255)                       cccacTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGCAGGTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATCTCTGGGCACcagc                                                                                                         >  1:1187799/1‑139 (MQ=255)                                 ccTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGcc                                                                                               >  2:765173/1‑139 (MQ=255)                                   tgctgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCt                                                                                             >  2:947234/1‑139 (MQ=255)                                      tgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                               <  2:1323625/102‑1 (MQ=255)                                      tgGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATg                                                                                                                               >  1:1323625/1‑102 (MQ=255)                                        gcggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                    <  2:576217/127‑1 (MQ=255)                                        gcggcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTa                                                                                                    >  1:576217/1‑127 (MQ=255)                                           gcACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAg                                                                                     >  2:1068580/1‑139 (MQ=255)                                                 aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                               <  2:910135/139‑1 (MQ=255)                                                 aaaCACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                               <  1:1068580/139‑1 (MQ=255)                                                           gCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGAc                                                                     <  2:163487/139‑1 (MQ=255)                                                               tAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGa                                                                                    <  1:412794/120‑1 (MQ=255)                                                               tAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGa                                                                                    >  2:412794/1‑120 (MQ=255)                                                                      gggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAAc                                                          <  1:947234/139‑1 (MQ=255)                                                                       ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                               >  1:562007/1‑117 (MQ=255)                                                                       ggCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCa                                                                               <  2:562007/117‑1 (MQ=255)                                                                        gCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                       <  2:1688959/108‑1 (MQ=255)                                                                        gCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGaaaa                                                                                       >  1:1688959/1‑108 (MQ=255)                                                                             tGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCCGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCgg                                                   >  2:56941/1‑139 (MQ=255)                                                                               gagaTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGc                                                 >  2:1644397/1‑139 (MQ=255)                                                                                  aTTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGt                                              >  2:1618037/1‑139 (MQ=255)                                                                                      tgAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                          >  2:1642306/1‑139 (MQ=255)                                                                                              cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcg                                                                                                           >  2:91486/1‑66 (MQ=255)                                                                                              cAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACcg                                                                                                           <  1:91486/66‑1 (MQ=255)                                                                                                     ccgcGATTATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTAGGCACCGGCGGTTAACGCCCTGGTAAAAGACGGCAAGGTCTTGACCGATTTTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                           <  2:1142429/139‑1 (MQ=255)                                                                                                           ttATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                               >  1:306422/1‑113 (MQ=255)                                                                                                           ttATCGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAg                                               <  2:306422/113‑1 (MQ=255)                                                                                                               cGCTGCCTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGggtg                 <  2:1342831/139‑1 (MQ=255)                                                                                                                    ccTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                           <  1:1557445/124‑1 (MQ=255)                                                                                                                    ccTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGa                           >  2:1557445/1‑124 (MQ=255)                                                                                                                     cTGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTgcgc           >  1:55816/1‑139 (MQ=255)                                                                                                                      tGGCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCa          <  2:55816/139‑1 (MQ=255)                                                                                                                       ggCAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTAGACGTCTGGGTGGTGCGCaa         >  2:1566662/1‑139 (MQ=255)                                                                                                                         cAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                          >  1:783799/1‑104 (MQ=255)                                                                                                                         cAGCGGGGAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTgggg                                          <  2:783799/104‑1 (MQ=255)                                                                                                                              gggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAtttt  >  1:1402691/1‑139 (MQ=255)                                                                                                                              gggAGGTATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGAtttt  >  1:999061/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                      GCCGTACCGTTTATCTCCACCACCCACTACAGCCTGCTGGCGGCACTGAAACACTGGGGCATTAAACCCGGGCAAGTGGAGATTGTGAACCTGCAGCCGCCCGCGATTATCGCTGCCTGGCAGCGGGGAGATATTGATGGTGCTTATGTCTGGGCACCGGCGGTTAACGCCCTGGAAAAAGACGGCAAGGTGTTGACCGATTCTGAACAGGTCGGGCAGTGGGGCGCGCCAACGCTGGACGTCTGGGTGGTGCGCAAAGATTTT  >  NC_000913/385607‑385870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑