Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	437,107	G→A	*173* (TAG→TAA)	pgpA →	phosphatidylglycerophosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	437,107	0	G	A	100.0%	219.8 / NA	68	*173* (TAG→TAA)	pgpA	phosphatidylglycerophosphatase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/36);  total (32/36)

TGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAC  >  NC_000913/436973‑437241                                                                                                                                       |                                                                                                                                       tGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgt                                                                                                                                    >  2:1176313/1‑139 (MQ=255)  ggCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgtg                                                                                                                                   >  1:911559/1‑139 (MQ=255)  ggCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgtg                                                                                                                                   <  2:785282/139‑1 (MQ=255)             tGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCt                                                                                                                        <  2:719673/139‑1 (MQ=255)                     tCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGAc                                                                                                                <  2:906946/139‑1 (MQ=255)                           tgtgCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                          <  2:379370/139‑1 (MQ=255)                               cATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                      >  1:1499171/1‑139 (MQ=255)                               cATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACg                                                                                                      <  1:1518795/139‑1 (MQ=255)                                   gcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCAcc                                                                                                                               <  2:274562/110‑1 (MQ=255)                                   gcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCAcc                                                                                                                               >  1:274562/1‑110 (MQ=255)                                   gcggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGtctc                                                                                                  >  2:545132/1‑139 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  2:46726/139‑1 (MQ=255)                                     ggcATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  1:1698840/139‑1 (MQ=255)                                       cATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTCTTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcatca                                                                                              >  2:501495/1‑139 (MQ=255)                                             gATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTg                                                                                        <  1:244956/139‑1 (MQ=255)                                             gATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTg                                                                                        <  2:274456/139‑1 (MQ=255)                                                 aTGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtgtg                                                                                                                  >  1:1651312/1‑109 (MQ=255)                                                 aTGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtgtg                                                                                                                  <  2:1651312/109‑1 (MQ=255)                                                 aTGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                    <  1:662517/139‑1 (MQ=255)                                                     tCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTg                                                                                        >  1:787651/1‑131 (MQ=255)                                                     tCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTg                                                                                        <  2:787651/131‑1 (MQ=255)                                                      cgacgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTAt                                                                               >  2:1521581/1‑139 (MQ=255)                                                        acgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTAttt                                                                             >  2:1118395/1‑139 (MQ=255)                                                         cgaTATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                            <  2:911559/139‑1 (MQ=255)                                                           atatTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTaaa                                                                          <  1:1521581/139‑1 (MQ=255)                                                                   gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                >  2:913709/1‑109 (MQ=255)                                                                   gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  1:913709/109‑1 (MQ=255)                                                                   gCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGa                                                                  >  1:84942/1‑139 (MQ=255)                                                                      ggggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAg                                                               >  1:1368234/1‑139 (MQ=255)                                                                       gggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACg                                                                   <  1:1306546/134‑1 (MQ=255)                                                                       gggTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACg                                                                   >  2:1306546/1‑134 (MQ=255)                                                                           gATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGc                                                          <  1:1176313/139‑1 (MQ=255)                                                                           gATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGc                                                          <  2:1736837/139‑1 (MQ=255)                                                                                 cGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGcc                                                                                                                         >  2:712160/1‑70 (MQ=255)                                                                                 cGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGcc                                                                                                                         <  1:712160/70‑1 (MQ=255)                                                                                   cAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTtata                                                  >  2:99188/1‑139 (MQ=255)                                                                                           cTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActct                                                                                  <  2:574530/99‑1 (MQ=255)                                                                                           cTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGActct                                                                                  >  1:574530/1‑99 (MQ=255)                                                                                                ttttATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGt                                     >  2:771687/1‑139 (MQ=255)                                                                                                 tttATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGtt                                    <  1:99188/139‑1 (MQ=255)                                                                                                    aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgtg                                                                                                                                   >  2:482689/1‑41 (MQ=255)                                                                                                    aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATtgtg                                                                                                                                   <  1:482689/41‑1 (MQ=255)                                                                                                    aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                >  1:1067171/1‑76 (MQ=255)                                                                                                    aTCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  2:1067171/76‑1 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtg                                                                                                                    >  1:102777/1‑55 (MQ=255)                                                                                                     tCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtg                                                                                                                    <  2:102777/55‑1 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtg                                                                                                                    >  1:1234944/1‑52 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGAtg                                                                                                                    <  2:1234944/52‑1 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                          <  2:900650/62‑1 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGt                                                                                                          >  1:900650/1‑62 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                             >  2:633820/1‑139 (MQ=255)                                                                                                        gTCATCACTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAAt                             <  1:501495/139‑1 (MQ=255)                                                                                                        gTCATCACTGGCCGCT‑GGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGCCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATg                                                           <  2:49081/108‑1 (MQ=39)                                                                                                        gTCATCACTGGCCGCT‑GGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGCCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATg                                                           >  1:49081/1‑108 (MQ=39)                                                                                                              aCTGGCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTc                       <  2:84942/139‑1 (MQ=255)                                                                                                                  gCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                    <  1:828535/74‑1 (MQ=255)                                                                                                                  gCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGAct                                                                                    >  2:828535/1‑74 (MQ=255)                                                                                                                  gCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                            >  2:1593235/1‑82 (MQ=255)                                                                                                                  gCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTa                                                                            <  1:1593235/82‑1 (MQ=255)                                                                                                                  gCCGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGc                   >  2:875110/1‑139 (MQ=255)                                                                                                                   ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                <  2:1648049/61‑1 (MQ=255)                                                                                                                   ccGCTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCtcat                                                                                                >  1:1648049/1‑61 (MQ=255)                                                                                                                      cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCa               <  2:1499171/139‑1 (MQ=255)                                                                                                                      cTGGGTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCa               <  2:332940/139‑1 (MQ=255)                                                                                                                        gggTATTCTGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCaaa             >  2:612079/1‑139 (MQ=255)                                                                                                                                tGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTc                                         <  1:333456/103‑1 (MQ=255)                                                                                                                                tGTCGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTc                                         >  2:333456/1‑103 (MQ=255)                                                                                                                                   cGTAATTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGTCTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAc  >  1:405086/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       TGGCCGATCCGCTGGTTTGATCGCAATGTGCATGGCGGCATGGGGATCATGATCGACGATATTGTCGCCGGGGTGATTTCCGCAGGCATCCTGTATTTTATCGGTCATCACTGGCCGCTGGGTATTCTGTCGTAGTTGTGCACCGATGCCTGATGTGACGCTTGTCACGTCTCATCAGGCCTGGACTCTTATTTAAATCCTACGACAGGATGCGGTTTATACGGCGTTTCCAGTTCGGCAATCTGTTCCGGCTTCAAAGTGATATCCAC  >  NC_000913/436973‑437241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑