Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 477,435 T→C T395A (ACT→GCT)  ylaB ← putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913477,4350TC100.0% 310.0 / NA 86T395A (ACT→GCT) ylaBputative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/42);  total (44/42)

TCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACG  >  NC_000913/477299‑477567
                                                                                                                                        |                                                                                                                                    
tCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGa                                                                                                                                    >  1:637655/1‑139 (MQ=255)
          gATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAgcgc                                                                                                                          >  2:1134701/1‑139 (MQ=255)
             ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:953816/139‑1 (MQ=255)
             ccTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:1725203/139‑1 (MQ=255)
               tGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtct                                                                                                                     >  2:313510/1‑139 (MQ=255)
               tGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtct                                                                                                                     >  1:370024/1‑139 (MQ=255)
                 tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGcgcg                                                                                                                         >  2:1578413/1‑133 (MQ=255)
                 tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGcgcg                                                                                                                         <  1:1578413/133‑1 (MQ=255)
                 tAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgt                                                                                                                   >  2:26044/1‑139 (MQ=255)
                       aCTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGa                                                                                                             >  1:1345489/1‑139 (MQ=255)
                        cTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  1:18457/139‑1 (MQ=255)
                        cTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  2:475284/139‑1 (MQ=255)
                        cTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGAt                                                                                                            <  1:1709789/139‑1 (MQ=255)
                           aaaCTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTaa                                                                                                         >  2:823210/1‑139 (MQ=255)
                                tGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  2:370024/139‑1 (MQ=255)
                                 gAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   <  1:313510/139‑1 (MQ=255)
                                 gAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   <  1:26044/139‑1 (MQ=255)
                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAg                                                                                                                             <  2:439671/105‑1 (MQ=255)
                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAg                                                                                                                             >  1:439671/1‑105 (MQ=255)
                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                                                  <  1:611688/116‑1 (MQ=255)
                                         cGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                                                  >  2:611688/1‑116 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtc                                                                                                                      >  2:791027/1‑110 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtc                                                                                                                      <  1:791027/110‑1 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  2:681269/128‑1 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  1:681269/1‑128 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattgat                                                                                         >  1:203772/1‑139 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattgat                                                                                         >  2:942312/1‑139 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgattgat                                                                                         >  2:1702528/1‑139 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTCCAAGCGCGATCTGTCTGGGAt                                                                                                            >  1:1450068/1‑120 (MQ=255)
                                           tACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTCCAAGCGCGATCTGTCTGGGAt                                                                                                            <  2:1450068/120‑1 (MQ=255)
                                            aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   <  2:421356/128‑1 (MQ=255)
                                            aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   >  1:421356/1‑128 (MQ=255)
                                            aCCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATc                                                                                        >  1:12906/1‑139 (MQ=255)
                                              cAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAgc                                                                                                                            >  1:1491578/1‑101 (MQ=255)
                                              cAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAgc                                                                                                                            <  2:1491578/101‑1 (MQ=255)
                                                 aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  2:824974/1‑122 (MQ=255)
                                                 aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  1:824974/122‑1 (MQ=255)
                                                 aaTCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTc                                                                                   >  2:1454888/1‑139 (MQ=255)
                                                   tcatcaAGATAAATTTCATGGCCCGCCTCCCGGTGGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  2:1600988/1‑101 (MQ=255)
                                                   tcatcaAGATAAATTTCATGGCCCGCCTCCCGGTGGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  1:1600988/101‑1 (MQ=255)
                                                      tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgat                                                                                             <  1:720150/124‑1 (MQ=255)
                                                      tcaAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgat                                                                                             >  2:720150/1‑124 (MQ=255)
                                                                        cccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGAtttttt                                                            >  1:1482620/1‑139 (MQ=255)
                                                                         ccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:120458/1‑79 (MQ=255)
                                                                         ccGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:120458/79‑1 (MQ=255)
                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                                                  >  1:460307/1‑83 (MQ=255)
                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAtctgtc                                                                                                                  <  2:460307/83‑1 (MQ=255)
                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc                                                          <  1:823210/139‑1 (MQ=255)
                                                                          cGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc                                                          <  1:1269342/139‑1 (MQ=255)
                                                                             cTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                                       <  2:637655/139‑1 (MQ=255)
                                                                               cccGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGt                                                     <  1:942312/139‑1 (MQ=255)
                                                                               cccGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGt                                                     <  2:12906/139‑1 (MQ=255)
                                                                                 cGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGa                                                   >  2:1257985/1‑139 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       >  1:176301/1‑65 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGAt                                                                                                                       <  2:176301/65‑1 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  2:562236/84‑1 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  1:1123311/1‑84 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  1:562236/1‑84 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  2:1123311/84‑1 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc                                                          <  1:396045/126‑1 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc                                                          >  2:396045/1‑126 (MQ=255)
                                                                                       cGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                             >  2:1397217/1‑139 (MQ=255)
                                                                                        gagaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACggg                                            <  2:203772/139‑1 (MQ=255)
                                                                                         agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                                       >  1:616079/1‑127 (MQ=255)
                                                                                         agaAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                                       <  2:616079/127‑1 (MQ=255)
                                                                                                aTCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   >  1:813126/1‑76 (MQ=255)
                                                                                                aTCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGAt                                                                                                   <  2:813126/76‑1 (MQ=255)
                                                                                                aTCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgat                                                                                             <  2:282741/82‑1 (MQ=255)
                                                                                                aTCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGtgat                                                                                             >  1:282741/1‑82 (MQ=255)
                                                                                                   ggggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGCTCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGACTTTTTcc                                                          <  1:1174884/114‑1 (MQ=255)
                                                                                                   ggggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTGCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTcc                                                          >  2:1174884/1‑114 (MQ=255)
                                                                                                     ggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                                       >  1:1525685/1‑115 (MQ=255)
                                                                                                     ggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAg                                                       <  2:1525685/115‑1 (MQ=255)
                                                                                                     ggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                             <  2:1100438/125‑1 (MQ=255)
                                                                                                     ggCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACgg                                             >  1:1100438/1‑125 (MQ=255)
                                                                                                      gcgcTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGa                              <  1:1702528/139‑1 (MQ=255)
                                                                                                         cTGGTTTTCGGATCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCg                           >  1:963451/1‑139 (MQ=255)
                                                                                                                     tCGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATgctgct               <  1:944781/139‑1 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    <  1:30650/53‑1 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGa                                                                                                    >  2:30650/1‑53 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGAtt                                       <  2:1649987/114‑1 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGAtt                                       >  1:1649987/1‑114 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGctgctg              >  1:1778349/1‑139 (MQ=255)
                                                                                                                      cGGCAAACTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGctgctg              >  2:533359/1‑139 (MQ=255)
                                                                                                                            aCTCGCGTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCTATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATg        <  2:1345489/139‑1 (MQ=255)
                                                                                                                                  gTTCAGCGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACg  >  1:1658532/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TCGACATCCAGATCCTGTAAATAACTTAAACTTGAATACCCCGTACCAAAATCATCAAGATAAATTTCATGGCCCGCCTCCCGGTAGCGAGAAATTATCGGGGCGCTGGTTTTCGGATCGGCAAACTCGCGTTCAGTGAGTTCAAGCGCGATCTGTCTGGGATTAACCTGATAGTGATTGATCATGTCACGCAGCAATTGCGGGATTTTTTCCGAGGTGAGCACGGGGGATTCAAGATTGATCGAAATATGCTGCTGTGGATGCTGACG  >  NC_000913/477299‑477567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑