Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,004,160 (G)7→6 coding (214/711 nt) ycbF → putative periplasmic pilin chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,004,1540G.100.0% 269.2 / NA 66coding (208/711 nt)ycbFputative periplasmic pilin chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (32/34);  total (32/34)

AGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAAC  >  NC_000913/1004020‑1004284
                                                                                                                                      |                                                                                                                                  
aggcaggAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg                                                                                                                                <  1:168065/139‑1 (MQ=255)
      gAGTGGTGGTCGGCGGAACACGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCg                                                                                                                         <  2:1641350/139‑1 (MQ=255)
                cggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg                                                                                                                                <  2:1482393/123‑1 (MQ=255)
                cggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg                                                                                                                                >  1:1482393/1‑123 (MQ=255)
                cggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGc                                                                                                               >  2:1407399/1‑139 (MQ=255)
                 ggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCagggg                                                                                                                                >  2:968472/1‑122 (MQ=255)
                 ggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCagggg                                                                                                                                <  1:968472/122‑1 (MQ=255)
                 ggcggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGcc                                                                                                              <  2:1481117/139‑1 (MQ=255)
                   cggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            >  2:325745/1‑139 (MQ=255)
                   cggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            >  2:352177/1‑139 (MQ=255)
                   cggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            <  2:168345/139‑1 (MQ=255)
                   cggAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            >  2:736661/1‑139 (MQ=255)
                                      ccGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                                                         <  1:325745/139‑1 (MQ=255)
                                      ccGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc                                                                                         <  1:736661/139‑1 (MQ=255)
                                               acagaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCa                                                                                                             >  2:569972/3‑110 (MQ=255)
                                               acagaATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCa                                                                                                             <  1:569972/108‑1 (MQ=255)
                                                    aTCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTcc                                                                           <  2:734030/139‑1 (MQ=255)
                                                      cGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                                                                         <  1:417354/139‑1 (MQ=255)
                                                        atatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGaa                                                                       >  1:471288/1‑139 (MQ=255)
                                                         tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                                                      <  1:352177/139‑1 (MQ=255)
                                                                       aCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGa                                                                                                                     >  1:399239/1‑78 (MQ=255)
                                                                       aCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGa                                                                                                                     <  2:399239/78‑1 (MQ=255)
                                                                         tAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                                                                         <  2:363584/120‑1 (MQ=255)
                                                                         tAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTg                                                                         >  1:363584/1‑120 (MQ=255)
                                                                            tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGt                                                   >  2:717530/1‑139 (MQ=255)
                                                                              ccAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTg                                                 <  1:276796/139‑1 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACa                                                                                                                   <  1:900898/70‑1 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACa                                                                                                                   >  2:900898/1‑70 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                                                      >  1:563767/1‑99 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc                                                                                      <  2:563767/99‑1 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGc                                                                                       >  1:409376/1‑98 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                                                      >  1:1430771/1‑115 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAg                                                                      <  2:1430771/115‑1 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcgc                                              >  1:175560/1‑139 (MQ=255)
                                                                                 gTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCATCGACAGTGCCAGCACCATTACTGGCCGCTCCGc                                                                                       <  2:409376/98‑1 (MQ=255)
                                                                                  tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCt                                             >  2:616470/1‑139 (MQ=255)
                                                                                         tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGcc                                                                                                              >  2:897101/1‑67 (MQ=255)
                                                                                         tCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGcc                                                                                                              <  1:897101/67‑1 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGcc                                                                                                              <  1:806563/64‑1 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGcc                                                                                                              >  2:806563/1‑64 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            >  2:1156944/1‑66 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            <  1:1156944/66‑1 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcgc                                              >  2:943543/1‑128 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTgcgc                                              <  1:943543/128‑1 (MQ=255)
                                                                                            tGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAAc                                   >  2:1227109/1‑139 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg                                                                                                                                <  2:665998/40‑1 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCggggg                                                                                                                                >  1:665998/1‑40 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg                                                                                        >  2:1125509/1‑79 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCg                                                                                        <  1:1125509/79‑1 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                                                            <  2:904430/107‑1 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGa                                                            >  1:904430/1‑107 (MQ=255)
                                                                                                   ttaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGc                            >  2:408443/1‑139 (MQ=255)
                                                                                                        aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAg                                                                                                            >  2:1124060/1‑54 (MQ=255)
                                                                                                        aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACAATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                       >  1:888528/1‑139 (MQ=255)
                                                                                                        aGTAAAATCAACCGCCCAACGCGTTGGGCCGGGAGGAAGCGTCGACAGTGCCAg                                                                                                            <  1:1124060/54‑1 (MQ=38)
                                                                                                          tAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                                                           <  2:84342/69‑1 (MQ=255)
                                                                                                          tAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCt                                                                                           >  1:84342/1‑69 (MQ=255)
                                                                                                          tAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCt                     <  2:471288/139‑1 (MQ=255)
                                                                                                          tAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCt                     <  1:616470/139‑1 (MQ=255)
                                                                                                             aaTCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                       >  1:777454/1‑134 (MQ=255)
                                                                                                             aaTCAACCGCCCAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACAt                       <  2:777454/134‑1 (MQ=255)
                                                                                                                      cccAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCa                                                                                                          >  1:1544730/1‑42 (MQ=38)
                                                                                                                      cccAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCa                                                                                                          <  2:1544730/42‑1 (MQ=38)
                                                                                                                      cccAACGCGTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATc         >  1:1586760/1‑139 (MQ=255)
                                                                                                                            gcgTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGaaa   <  2:1586760/139‑1 (MQ=255)
                                                                                                                            gcgTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGaaa   <  1:1227109/139‑1 (MQ=255)
                                                                                                                             cgTTGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAAc  <  2:721862/139‑1 (MQ=255)
                                                                                                                                tGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                                                               >  2:1284913/1‑59 (MQ=255)
                                                                                                                                tGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAtt                                                                               <  1:1284913/59‑1 (MQ=255)
                                                                                                                                tGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGaa                                    <  1:1345337/102‑1 (MQ=255)
                                                                                                                                tGGGCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGaa                                    >  2:1345337/1‑102 (MQ=255)
                                                                                                                                 gggCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGaaa   <  1:860405/134‑1 (MQ=255)
                                                                                                                                 gggCCGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGaaa   >  2:860405/1‑134 (MQ=255)
                                                                                                                                      |                                                                                                                                  
AGGCAGGAGTGGTGGTCGGCGGAACGCGATTTATCTTTCCGGCAGACAGAGAATCGATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCTCCTGAAGCCCGGTACGACAGGCACGTTGCGCTTGCTGAGAACGGAAAGCGACATCTTGCCTGTGGATCGCGAAAC  >  NC_000913/1004020‑1004284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: