Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,370,0360T.100.0% 211.1 / NA 52intergenic (+33/‑180)pspE/ycjMthiosulfate:cyanide sulfurtransferase (rhodanese)/alpha amylase catalytic domain family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (25/27);  total (25/27)

CAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGT  >  NC_000913/1369911‑1370159
                                                                                                                             |                                                                                                                           
caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                               <  1:826251/139‑1 (MQ=255)
 aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                              <  1:662815/139‑1 (MQ=255)
   gcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                            >  2:897311/1‑139 (MQ=255)
    caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACAcc                                                                                                           <  2:839538/139‑1 (MQ=255)
      aaaGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCtt                                                                                                         <  2:1285595/139‑1 (MQ=255)
             tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt                                                                                                                        >  2:1135429/1‑118 (MQ=255)
             tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttt                                                                                                                        <  1:1135429/118‑1 (MQ=255)
             tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACa                                                                                                  <  1:890803/139‑1 (MQ=255)
                 tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATccc                                                                                              >  1:245837/1‑139 (MQ=255)
                 tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATccc                                                                                              >  2:816942/1‑139 (MQ=255)
                   gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCCAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                              >  1:1393164/1‑121 (MQ=255)
                   gCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCCAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                              <  2:1393164/121‑1 (MQ=255)
                            gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                   >  2:1121068/1‑123 (MQ=255)
                            gATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                   <  1:1121068/123‑1 (MQ=255)
                                 aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                              >  1:819396/1‑139 (MQ=255)
                                 aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTCATTTACATCCCCATAGATTATTTGCGt                                                                              <  2:1285757/139‑1 (MQ=255)
                                     aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                              >  2:1191233/1‑103 (MQ=255)
                                     aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTcac                                                                                                              <  1:1191233/103‑1 (MQ=255)
                                      cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                         >  2:1461527/1‑139 (MQ=255)
                                          gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                              >  1:774846/1‑130 (MQ=255)
                                          gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                              <  2:774846/130‑1 (MQ=255)
                                          gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcaca                                                                     >  2:1468112/1‑139 (MQ=255)
                                           agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                               <  1:564014/96‑1 (MQ=255)
                                           agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                               >  2:564014/1‑96 (MQ=255)
                                           agaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACaa                                                                    >  2:739871/1‑139 (MQ=255)
                                                                  aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                            >  1:1518866/1‑92 (MQ=255)
                                                                  aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCa                                                                                            <  2:1518866/92‑1 (MQ=255)
                                                                    cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                           <  1:586332/139‑1 (MQ=255)
                                                                      cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt                                                                                                                      <  2:439991/63‑1 (MQ=255)
                                                                      cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGtttttttt                                                                                                                      >  1:439991/1‑63 (MQ=255)
                                                                      cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                               <  2:740246/69‑1 (MQ=255)
                                                                      cAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTca                                                                                                               >  1:740246/1‑69 (MQ=255)
                                                                              aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                 <  1:362179/107‑1 (MQ=255)
                                                                              aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATa                                                                 >  2:362179/1‑107 (MQ=255)
                                                                                ggTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                               <  1:1461527/139‑1 (MQ=255)
                                                                                   cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                            >  2:819409/1‑139 (MQ=255)
                                                                                     aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                            >  2:924173/1‑57 (MQ=255)
                                                                                     aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                            <  1:924173/57‑1 (MQ=255)
                                                                                        gTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTaaaa                       >  2:299390/1‑139 (MQ=255)
                                                                                           aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                            <  2:1256982/51‑1 (MQ=255)
                                                                                           aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCacac                                                                                                            >  1:1256982/1‑51 (MQ=255)
                                                                                           aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                    <  1:897311/139‑1 (MQ=255)
                                                                                           aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                    <  2:245837/139‑1 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTAtt                                                                                                      >  2:929684/1‑50 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTAtt                                                                                                      <  1:929684/50‑1 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                   <  1:1060647/53‑1 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTAc                                                                                                   >  2:1060647/1‑53 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                               <  2:1390062/121‑1 (MQ=255)
                                                                                                  ttCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGt                               >  1:1390062/1‑121 (MQ=255)
                                                                                                             cggcgCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaat                                            <  2:1563757/97‑2 (MQ=255)
                                                                                                             cggcgCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaat                                            >  1:1563757/1‑96 (MQ=255)
                                                                                                             cggcgCTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt  <  1:1468112/139‑1 (MQ=255)
                                                                                                               gcgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATcccc                                                                                             >  2:1166386/1‑46 (MQ=255)
                                                                                                               gcgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATcccc                                                                                             <  1:1166386/46‑1 (MQ=255)
                                                                                                                cgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg                                                   >  2:1094241/1‑87 (MQ=255)
                                                                                                                cgcTTATGCGCCGTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTgg                                                   <  1:1094241/87‑1 (MQ=255)
                                                                                                                             |                                                                                                                           
CAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTTTTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGT  >  NC_000913/1369911‑1370159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: