Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,655,360 (C)5→4 coding (314/327 nt) ynfA ← UPF0060 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,655,3560C.100.0% 209.1 / NA 52coding (318/327 nt)ynfAUPF0060 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/25);  total (27/25)

AAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAA  >  NC_000913/1655220‑1655488
                                                                                                                                        |                                                                                                                                    
aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                    <  1:1574549/139‑1 (MQ=255)
          aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                         <  1:1634202/139‑1 (MQ=255)
          aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                         <  1:681688/139‑1 (MQ=255)
          aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                         <  1:13427/139‑1 (MQ=255)
            tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       <  2:582597/139‑1 (MQ=255)
            tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       <  2:713553/139‑1 (MQ=255)
                   aaaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCa                                                                                                                <  1:80361/139‑1 (MQ=255)
                             gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                      >  2:991171/1‑139 (MQ=255)
                                         tgtgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCaa                                                                                          <  2:1182550/139‑1 (MQ=255)
                                           tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                               <  2:1040593/101‑5 (MQ=255)
                                           tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                               >  1:1040593/1‑97 (MQ=255)
                                                   tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                <  2:464913/139‑1 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       >  1:1540522/1‑98 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       <  2:1540522/98‑1 (MQ=255)
                                                     aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAg                                                                             >  1:78868/1‑139 (MQ=255)
                                                                      aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                <  2:461953/120‑1 (MQ=255)
                                                                      aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                >  1:461953/1‑120 (MQ=255)
                                                                          cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                               >  2:857851/1‑66 (MQ=255)
                                                                          cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccccagcc                                                                                                                               <  1:857851/70‑5 (MQ=255)
                                                                          cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       >  1:837670/1‑77 (MQ=255)
                                                                          cTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaaca                                                                                                                       <  2:837670/77‑1 (MQ=255)
                                                                            tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                      >  2:79599/1‑92 (MQ=255)
                                                                            tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                      <  1:79599/92‑1 (MQ=255)
                                                                            tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                       >  1:1174733/1‑139 (MQ=255)
                                                                             ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                        <  1:1177543/105‑1 (MQ=255)
                                                                             ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                        >  2:1177543/1‑105 (MQ=255)
                                                                              tGTCAGCTGACACTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCAt                                                    <  2:78868/139‑1 (MQ=255)
                                                                               gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                     >  2:528020/1‑106 (MQ=255)
                                                                               gTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                     <  1:528020/106‑1 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCg                                                                                                                            <  2:343247/62‑1 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCg                                                                                                                            >  1:343247/1‑62 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                        <  1:109528/130‑1 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                        >  2:109528/1‑130 (MQ=255)
                                                                                    cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                               >  2:368971/1‑139 (MQ=255)
                                                                                       acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                      <  1:1330931/97‑1 (MQ=255)
                                                                                       acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                      >  2:1330931/1‑97 (MQ=255)
                                                                                             ccTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                    >  1:1184607/1‑46 (MQ=255)
                                                                                             ccTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCGccc                                                                                                                                    <  2:1184607/46‑1 (MQ=255)
                                                                                                 ttatcttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCcaca                                  >  1:709151/1‑139 (MQ=255)
                                                                                                     cttatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACACGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCaa                              >  2:981105/1‑139 (MQ=255)
                                                                                                       tatTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                            >  1:1476906/1‑139 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                         >  2:200602/1‑45 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGcaa                                                                                                                         <  1:200602/45‑1 (MQ=255)
                                                                                                        atTGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGc                           >  1:499693/1‑139 (MQ=255)
                                                                                                         ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                            <  1:666738/137‑1 (MQ=255)
                                                                                                         ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                            >  2:666738/1‑137 (MQ=255)
                                                                                                         ttGATAAAATGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCg                         >  2:396706/1‑139 (MQ=255)
                                                                                                              aaaaTGGATTTATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                     >  1:557766/1‑139 (MQ=255)
                                                                                                                      tttATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa             >  1:680835/1‑139 (MQ=255)
                                                                                                                        tATGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGGCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg           <  2:1174733/139‑1 (MQ=255)
                                                                                                                         aTGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGc          <  1:367229/139‑1 (MQ=255)
                                                                                                                          tGTTCTTACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGcc         >  2:679271/1‑139 (MQ=255)
                                                                                                                               ttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt    >  2:1648720/2‑139 (MQ=255)
                                                                                                                               ttACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt    >  1:64307/2‑139 (MQ=255)
                                                                                                                                tACGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATa   <  1:368971/139‑1 (MQ=255)
                                                                                                                                 aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                >  2:275048/1‑61 (MQ=255)
                                                                                                                                 aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                <  1:275048/61‑1 (MQ=255)
                                                                                                                                 aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCCGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa  <  1:679271/139‑1 (MQ=255)
                                                                                                                                 aCGTGCGCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATaa  >  2:1267457/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                    
AAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCCCCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAA  >  NC_000913/1655220‑1655488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: