Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,850,810 (C)5→4 intergenic (+117/‑50) sppA → / → ansA protease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,8060C.100.0% 147.8 / NA 37intergenic (+113/‑54)sppA/ansAprotease IV (signal peptide peptidase)/cytoplasmic L‑asparaginase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (18/19);  total (18/19)

TGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGT  >  NC_000913/1850676‑1850939
                                                                                                                                  |                                                                                                                                     
tGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                              <  1:468468/139‑1 (MQ=255)
  cgcCAACATGCGTGAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCGGCATAGTATCAGGTGCGCTCCCCTGCCTCa                                                                                                                            >  2:481591/1‑139 (MQ=255)
            cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccct                                                                                                                                   <  2:680193/123‑2 (MQ=255)
            cGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccct                                                                                                                                   >  1:680193/1‑122 (MQ=255)
                aaGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTg                                                                                                              <  1:876184/139‑1 (MQ=255)
                     ttGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccctgc                                                                                                                                 <  1:1234742/116‑4 (MQ=255)
                     ttGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcccctgc                                                                                                                                 >  2:1234742/1‑113 (MQ=255)
                      tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                        >  2:669889/1‑139 (MQ=255)
                      tGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACAAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCttt                                                                                                        >  2:1440564/1‑139 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc                                                                                                                                      >  2:605477/1‑103 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTcc                                                                                                                                      <  1:605477/103‑1 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                              <  1:1225390/110‑1 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCt                                                                                                                              >  2:1225390/1‑110 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCAc                                                                                                                           <  2:1506757/113‑1 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCAc                                                                                                                           >  1:1506757/1‑113 (MQ=255)
                             aGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtc                                                                                                 >  2:720586/1‑139 (MQ=255)
                                ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              <  1:669889/139‑1 (MQ=255)
                                ggCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              <  1:720586/139‑1 (MQ=255)
                                  ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCAc                                                                                                                           >  2:1462663/1‑108 (MQ=255)
                                  ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCAc                                                                                                                           <  1:1462663/108‑1 (MQ=255)
                                  ccGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                            >  2:1168183/1‑139 (MQ=255)
                                          tcgtcgGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCatatcaata                                                                                    <  2:1555206/139‑1 (MQ=255)
                                                gCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGc                                                                              >  2:1565796/1‑139 (MQ=255)
                                                                  tCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                            <  2:108251/139‑1 (MQ=255)
                                                                   cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCTTAGTATCAGGTGCGCTCACCTGCCTAACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              <  2:1218272/104‑1 (MQ=255)
                                                                   cgacgaATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              >  1:1218272/1‑104 (MQ=255)
                                                                          tCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTac                                                    <  1:609947/139‑1 (MQ=255)
                                                                               ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                            >  2:290114/1‑94 (MQ=255)
                                                                               ttCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcattca                                                                                            <  1:290114/94‑1 (MQ=255)
                                                                                 cccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              >  2:1464810/1‑90 (MQ=255)
                                                                                 cccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              <  1:1464810/90‑1 (MQ=255)
                                                                                  ccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcgg                                            <  1:1565796/139‑1 (MQ=255)
                                                                                  ccGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGCATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACAcgggcgg                                            <  1:1021215/139‑1 (MQ=255)
                                                                                     cTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGAc                                         >  2:1619158/1‑139 (MQ=255)
                                                                                                                 aTAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                            <  2:1237257/92‑1 (MQ=255)
                                                                                                                 aTAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACg                                                            >  1:1237257/1‑92 (MQ=255)
                                                                                                                 aTAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt             >  1:1612367/1‑139 (MQ=255)
                                                                                                                 aTAGTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGt             >  1:1449314/1‑139 (MQ=255)
                                                                                                                    gTATCAGGTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTtat          <  2:1449314/139‑1 (MQ=255)
                                                                                                                          ggTGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCg    >  1:810629/1‑139 (MQ=255)
                                                                                                                            tGCGCTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGgt  <  1:1619158/139‑1 (MQ=255)
                                                                                                                             gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              <  2:50023/46‑1 (MQ=255)
                                                                                                                             gcgcTCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATAtcatt                                                                                              >  1:50023/1‑46 (MQ=255)
                                                                                                                                  |                                                                                                                                     
TGCGCCAACATGCGTTAAGTCTTGTACTGAGTGGCCGACAGATCGTCGGCCACATTATTTTTTACGTCGACGAATCCTCTTCCCGCTGTTTCGCCCCATATTTCCTTATCCGCATAGTATCAGGTGCGCTCCCCCTGCCTCACGTATATACTTTTGCTCTTTCGATATCATTCATATCAATATCATGCAAAAGAAATCAATTTACGTTGCCTACACGGGCGGGACCATCGGGATGCAGCGTTCCGAGCAGGGTTATATACCGGT  >  NC_000913/1850676‑1850939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: