| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,511,746 | (A)8→9 | coding (599/906 nt) | yhfZ ← | putative DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,511,738 | 1 | . | A | 94.3% | 162.5 / 2.0 | 53 | coding (607/906 nt) | yhfZ | putative DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base A (28/22); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC > NC_000913/3511604‑3511871 | cgTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑aa‑aa > 1:858777/1‑139 (MQ=255) ccAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCa < 2:55090/139‑1 (MQ=255) ggCATGGTCAGTTCGTTTTCGGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGGAAACTCTCGTGATAAGAGAGTTCGACTCGTTCCACATCACTACCGCC‑‑AATAAAAAA‑CATCGGTCATg < 2:98482/138‑1 (MQ=255) aTGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGAtt < 1:208651/139‑1 (MQ=255) ggTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGAtttt > 2:194411/1‑139 (MQ=255) tCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCt > 2:1179982/1‑139 (MQ=255) gTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGAt > 1:636009/1‑139 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATg < 1:1097595/123‑1 (MQ=255) tttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATg > 2:1097595/1‑123 (MQ=255) ttCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAAAA‑CATCGGTCATGATTTTCTGATccgccg < 2:858777/139‑1 (MQ=255) gCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACgg < 1:1593786/139‑1 (MQ=255) gATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AA‑AAAAAACCATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAc > 2:932185/1‑139 (MQ=255) aTCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 2:1034516/1‑139 (MQ=255) aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTc > 2:141810/1‑97 (MQ=255) aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTc < 1:141810/97‑1 (MQ=255) aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg > 1:915318/1‑136 (MQ=255) aCCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCAcg < 2:915318/136‑1 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 2:994157/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:538464/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 1:1648660/1‑139 (MQ=255) cGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CACCGGACATGAATTTCTCATCCACCCAACCGTTATCCAGACCCAccagctc > 1:505023/1‑133 (MQ=255) cGCATCGACATCGCCTTGAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACAACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 2:948884/1‑139 (MQ=255) cATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACCCGCTTCa > 1:1377721/1‑139 (MQ=255) tCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCg < 1:1034516/139‑1 (MQ=255) cGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCgg < 2:1548491/139‑1 (MQ=255) gCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:948884/139‑1 (MQ=255) cTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AA‑AAAAAACCATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:932185/139‑1 (MQ=255) tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:1417421/1‑134 (MQ=255) tAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:1417421/134‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 2:203625/128‑1 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 1:203625/1‑128 (MQ=255) tGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTAc > 1:885405/1‑139 (MQ=255) tAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:1103257/1‑121 (MQ=255) tAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:1103257/121‑1 (MQ=255) tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt < 1:459967/117‑1 (MQ=255) tcGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCCAAAA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACt > 2:459967/1‑117 (MQ=255) aTAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTc < 2:1648660/139‑1 (MQ=255) tAAGAGAGATCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCg < 2:885405/139‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATccg > 2:453634/1‑60 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATccg < 1:453634/60‑1 (MQ=255) aTCGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTa > 2:1141498/1‑139 (MQ=255) cGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa < 1:1297498/98‑1 (MQ=255) cGACTCGTTCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGa > 2:1297498/1‑98 (MQ=255) tCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt < 1:927369/78‑1 (MQ=255) tCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCtt > 2:927369/1‑78 (MQ=255) tCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg < 2:477756/117‑1 (MQ=255) tCCACATCACTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTg > 1:477756/1‑117 (MQ=255) cTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg < 1:1327971/128‑1 (MQ=255) cTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCg > 2:1327971/1‑128 (MQ=255) cTACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTcc > 1:254089/1‑139 (MQ=255) tACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc < 2:1233245/94‑1 (MQ=255) tACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGc > 1:1233245/1‑94 (MQ=255) tACCGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCa > 1:36730/1‑139 (MQ=255) ccGCCA‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAgc > 1:228413/1‑139 (MQ=255) | CGTCGCCTCTAATCCCAGCATGGTCAGTTCGTTTTCCGCCACCACGTTCCAGATCACCGCATCGACATCGCCTTTAACAATGCGTTGTAAACTCTCGTGATAAGAGAGATCGACTCGTTCCACATCACTACCGCC‑‑AA‑AAAAAA‑CATCGGTCATGATTTTCTGATCCGCCGAACGGTTATCCAGCCCCACGCGCTTCACGTTTGCGGACTCGCCTTTACGGCAAATCAACTGGTGCTCGCCAACGTAGGTGTGCGGCCCCAACTCCAGC > NC_000913/3511604‑3511871 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |