Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,664,3572.G38.0% 66.4 / 185.7 108coding (262/729 nt)gadWtranscriptional activator of gadA and gadBC, repressor of gadX
Reads supporting (aligned to +/- strand):  ref base . (35/32);  new base G (20/21);  total (55/53)
Fisher's exact test for biased strand distribution p-value = 8.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑GGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAT  >  NC_000913/3664219‑3664479
                                                                                                                                            |                                                                                                                            
tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                <  1:1399760/139‑1 (MQ=255)
tGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAt                                                                                                                                <  2:1443887/139‑1 (MQ=255)
   tATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑ggg                                                                                                                           <  2:1289130/139‑1 (MQ=255)
    aTTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg                                                                                                                          >  1:929841/1‑139 (MQ=255)
         aaaGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg‑‑ggggt                                                                                                                   <  1:180881/139‑4 (MQ=255)
            gTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACt                                                                                                                 <  1:795929/139‑1 (MQ=255)
             tAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTg                                                                                                                >  2:1177928/1‑139 (MQ=255)
               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGt                                                                                                              >  1:298238/1‑139 (MQ=255)
               aGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGt                                                                                                              >  1:1179259/1‑139 (MQ=255)
                    aTCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGttaatt                                                                                                         <  2:929841/139‑1 (MQ=255)
                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAATGGTAGAGCATGTTGCTCAGTATCGGTCAATTTACCCCGGATTCATTGAAGTTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAAc                                                                                                      >  1:1051717/1‑139 (MQ=255)
                       aGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAAc                                                                                                      >  1:559100/1‑139 (MQ=255)
                           ctttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAAc                                                                                                  <  2:527870/139‑1 (MQ=255)
                            tttcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAAc                                                                                                  <  1:132382/139‑1 (MQ=255)
                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACgg                                                                                                >  1:69373/1‑139 (MQ=255)
                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               <  1:1300165/139‑1 (MQ=255)
                              tcttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               >  1:1394573/1‑139 (MQ=255)
                               cttATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               <  2:270443/139‑1 (MQ=255)
                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCttt                                                                                           >  1:1538670/1‑139 (MQ=255)
                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCttt                                                                                           >  2:484885/1‑139 (MQ=255)
                                   tGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  1:1255055/1‑139 (MQ=255)
                                    gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  2:690559/139‑1 (MQ=255)
                                    gagaAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  2:1177658/139‑1 (MQ=255)
                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:559100/139‑1 (MQ=255)
                                        aaaCATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  2:61170/1‑139 (MQ=255)
                                              cGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  1:774874/1‑134 (MQ=255)
                                              cGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAAcc                                                                                <  2:1538670/139‑1 (MQ=255)
                                                             gTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTa                                                                 >  2:1029154/1‑139 (MQ=255)
                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                              >  1:921054/1‑139 (MQ=255)
                                                                gagCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                              >  1:765710/1‑139 (MQ=255)
                                                                 agCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGa                                                            <  1:951106/139‑1 (MQ=255)
                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:693756/113‑1 (MQ=255)
                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  1:693756/1‑113 (MQ=255)
                                                                  gCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGAc                                                           >  2:88952/1‑139 (MQ=255)
                                                                      atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGttaa                                                                                                           >  1:362420/1‑87 (MQ=255)
                                                                      atTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGttaa                                                                                                           <  2:362420/87‑1 (MQ=255)
                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                      >  1:885411/1‑139 (MQ=255)
                                                                       ttGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                      >  1:1403245/1‑139 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAata                                                      >  1:14141/1‑139 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGt                                                                     >  1:655235/1‑123 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                              >  2:1465602/1‑130 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCt                                                              <  1:1465602/130‑1 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg‑‑gg                                                                                                                      >  1:1271044/1‑73 (MQ=255)
                                                                        tGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg‑‑gg                                                                                                                      <  2:1271044/73‑1 (MQ=255)
                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTgtgt                                                                                    >  1:856317/1‑106 (MQ=255)
                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTgtgt                                                                                    <  2:856317/106‑1 (MQ=255)
                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                         >  1:1468426/1‑133 (MQ=255)
                                                                          cTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGa                                                         <  2:1468426/133‑1 (MQ=255)
                                                                             aGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgt                                                >  1:1261115/1‑139 (MQ=255)
                                                                              gTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgta                                               <  1:1177928/139‑1 (MQ=255)
                                                                               tATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaa                                              >  1:788799/1‑139 (MQ=255)
                                                                                  cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  1:117919/92‑1 (MQ=255)
                                                                                  cGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  2:117919/1‑92 (MQ=255)
                                                                                   ggTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagta                                           <  2:69373/139‑1 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGt                                                                                                              >  1:1148403/1‑71 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGt                                                                                                              <  2:1148403/71‑1 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTgtaagtaa                                          >  1:1283708/1‑139 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACt                                                                                                                 >  1:769260/1‑67 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACt                                                                                                                 <  2:769260/67‑1 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGttaatt                                                                                                         <  2:1529185/75‑1 (MQ=255)
                                                                                    gTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGttaatt                                                                                                         >  1:1529185/1‑75 (MQ=255)
                                                                                       aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                      <  2:1051717/139‑1 (MQ=255)
                                                                                       aaTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                      <  1:106358/139‑1 (MQ=255)
                                                                                        aTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                     <  2:1394573/139‑1 (MQ=255)
                                                                                        aTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                     <  2:1428977/139‑1 (MQ=255)
                                                                                        aTTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑GGGGGCGGTGCCTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGc                                      <  2:534431/139‑1 (MQ=255)
                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                     >  1:1362014/1‑139 (MQ=255)
                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                     <  2:1362014/139‑1 (MQ=255)
                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          >  1:849606/1‑85 (MQ=255)
                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTg                                                                                          <  2:849606/85‑1 (MQ=255)
                                                                                         tttACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCaa                                    >  1:618041/1‑139 (MQ=255)
                                                                                             ccccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACt                                 >  2:354684/1‑139 (MQ=255)
                                                                                              cccGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               <  1:984420/75‑1 (MQ=255)
                                                                                                 ggATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCa                                     <  2:740018/131‑1 (MQ=255)
                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               >  1:171140/1‑70 (MQ=255)
                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGt                                                                                               <  2:171140/70‑1 (MQ=255)
                                                                                                   aTTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          <  2:1261115/139‑1 (MQ=255)
                                                                                                    ttCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          <  1:1459200/139‑1 (MQ=255)
                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGTATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  2:635689/1‑72 (MQ=255)
                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGTATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  1:635689/72‑1 (MQ=255)
                                                                                                            aGCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGatat                 >  1:698934/1‑139 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGatat                 >  2:884409/1‑139 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                       <  2:1659025/100‑1 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAat                                                       >  1:1659025/1‑100 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATc                >  2:3082/1‑139 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg‑‑gggtga                                                                                                                  >  2:1423578/1‑36 (MQ=255)
                                                                                                             gCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑gggg‑‑gggtga                                                                                                                  <  1:1423578/40‑5 (MQ=39)
                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAAc                                                                                                  <  2:476576/54‑1 (MQ=255)
                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAAc                                                                                                  >  1:476576/1‑54 (MQ=255)
                                                                                                                 tCAAAAATCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgt            <  2:1179259/139‑1 (MQ=255)
                                                                                                                   aaaaaTCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCTTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtc           <  1:1028202/139‑1 (MQ=255)
                                                                                                                     aaaTCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTGAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt         <  1:1029154/139‑1 (MQ=255)
                                                                                                                     aaaTCGCCGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAgtcgt         <  1:1011327/139‑1 (MQ=255)
                                                                                                                        tCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAGCTGTCAGCt                          <  1:1111418/118‑1 (MQ=255)
                                                                                                                        tCGCCGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCt                          >  2:1111418/1‑118 (MQ=255)
                                                                                                                           ccGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     >  1:325531/1‑57 (MQ=255)
                                                                                                                           ccGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCtgtg                                                                                     <  2:325531/57‑1 (MQ=255)
                                                                                                                           ccGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACg                                                          <  1:1139984/84‑1 (MQ=255)
                                                                                                                           ccGTCACCAGCGGCATGGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACg                                                          >  2:1139984/1‑84 (MQ=255)
                                                                                                                           ccGTCACCAGCGGCATGGGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAt  >  1:1499543/1‑139 (MQ=255)
                                                                                                                                            |                                                                                                                            
TGCTATTGAAAAGTAAGGGGATCAGTTCTTTCTTATGAGAAAACATCGATAAACAGGAAAGGTAGAGCATATTGCTCAGTATCGGTGAATTTACCCCGGATTCATTGAAGCTATCAAAAATCGCCGTCACCAGCGGCAT‑‑GGGG‑‑GGTGACTGGTTAATTAACAAACGGTCTTTGCTGTGTAACCCTAAATTGCGTGGTAGCTGACGAATATTTTGTAAGTAATGGCAAACTGTCAGCTCATCGATATCCAGTCGTCGCGTAT  >  NC_000913/3664219‑3664479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: