Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,745,5430AG54.5% 46.3 / 143.5 123Y247C (TAT→TGT) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
Reads supporting (aligned to +/- strand):  ref base A (27/29);  new base G (33/34);  total (60/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 125 total aligned reads displayed.

CGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGATTAC  >  NC_000913/3745417‑3745681
                                                                                                                              |                                                                                                                                          
cGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGt                                                                                                                                <  2:1639376/139‑1 (MQ=255)
cGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGt                                                                                                                                >  1:448446/1‑139 (MQ=255)
   tGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGc                                                                                                                             <  1:1653204/139‑1 (MQ=255)
     cAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           <  2:1569280/139‑1 (MQ=255)
             cTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTAtt                                                                                                                   >  2:1140253/1‑139 (MQ=255)
                gCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTAc                                                                                                                <  2:124303/139‑1 (MQ=255)
                          gTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTTTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                                                                           >  2:293127/1‑118 (MQ=255)
                          gTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                                                                           <  1:293127/118‑1 (MQ=255)
                           tctcCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGAc                                                                                                     <  1:226684/139‑1 (MQ=255)
                             tcCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGAc                                                                                                     <  2:792634/137‑1 (MQ=255)
                             tcCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGAc                                                                                                     >  1:792634/1‑137 (MQ=255)
                               cGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTc                                                                                                 >  2:1007059/1‑139 (MQ=255)
                               cGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATgcg                                                                                                                                        >  1:1327316/1‑100 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           >  2:38341/1‑112 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           <  1:38341/112‑1 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCg                                                                                                >  2:1019204/1‑139 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCg                                                                                                >  2:1287304/1‑139 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCg                                                                                                >  1:1272029/1‑139 (MQ=255)
                                ggTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCCGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCg                                                                                                >  2:340259/1‑139 (MQ=255)
                                ggTATCTGGGCGCCGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCg                                                                                                >  1:117367/1‑139 (MQ=255)
                                  tATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                                                                           <  1:845761/110‑1 (MQ=255)
                                  tATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                                                                           >  2:845761/1‑110 (MQ=255)
                                       gggCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                                                                         <  1:521485/139‑1 (MQ=255)
                                        ggCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACtt                                                                                        >  2:118023/1‑139 (MQ=255)
                                        ggCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGCTATTTACCGTGAAATGACCTTCGCCACACtt                                                                                        >  1:1321032/1‑139 (MQ=255)
                                         gcgcTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACttt                                                                                       >  2:1497420/1‑139 (MQ=255)
                                         gcgcTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACttt                                                                                       <  1:758174/139‑1 (MQ=255)
                                          cgcTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGt                                                                                                                                <  1:232709/97‑1 (MQ=255)
                                          cgcTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGt                                                                                                                                >  2:232709/1‑97 (MQ=255)
                                            cTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTgcgc                                                                                                                                       <  2:1530715/88‑1 (MQ=255)
                                            cTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCcac                                                                                                                          <  2:1365796/101‑1 (MQ=255)
                                            cTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCcac                                                                                                                          >  1:1365796/1‑101 (MQ=255)
                                            cTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                                                                                    <  1:1140253/139‑1 (MQ=255)
                                            cTGTTTCTTCCAGTCATCATTATTGGCGGGATTCGCTCCGGCCTGTTTAACCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTgcgc                                                                                                                                       >  1:1530715/1‑88 (MQ=255)
                                                       aGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCAtt                                                                         >  2:119672/1‑139 (MQ=255)
                                                          catcatTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgc                                                                      <  2:232566/139‑1 (MQ=255)
                                                           atcatTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTTTCGCCACAGGTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTTgcg                                                                     >  2:1151538/1‑139 (MQ=255)
                                                           atcatTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                                                                     <  1:119672/139‑1 (MQ=255)
                                                           atcatTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                                                                     >  1:1267934/1‑139 (MQ=255)
                                                             catTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGcgcg                                                                   >  1:915632/1‑139 (MQ=255)
                                                              attattGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCgcgg                                                                  <  1:19227/139‑1 (MQ=255)
                                                                   tGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCgcggcg                                                                >  1:258723/1‑136 (MQ=255)
                                                                   tGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCgcggcg                                                                <  2:258723/136‑1 (MQ=255)
                                                                   tGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGaaa                                                             >  2:777011/1‑139 (MQ=255)
                                                                     gCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACttt                                                                                       >  2:12053/1‑111 (MQ=255)
                                                                     gCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACttt                                                                                       <  1:12053/111‑1 (MQ=255)
                                                                     gCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAac                                                           <  1:1019204/139‑1 (MQ=255)
                                                                      cGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTTCGCCGTTTGTCGCCACAGTTATTTACCGTGAAAt                                                                                                        >  2:1039183/1‑93 (MQ=255)
                                                                       gggTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           <  2:1007890/73‑1 (MQ=255)
                                                                       gggTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           >  1:1007890/1‑73 (MQ=255)
                                                                       gggTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                                                                         >  1:754950/1‑107 (MQ=255)
                                                                       gggTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACt                                                                                         <  2:754950/107‑1 (MQ=255)
                                                                          tttCGGTCCGGCCTGTTTAACCCTACCGAAGTCGGGGGCGTTGCTGCTTTCCGTGCGCACATTGTCGCCACAGTTTTTTACCGTGAAATGACCTTCGCCACACTTTGGCCTGTTATCATTGGCGCGGCGAAAACCACCt                                                      >  2:1387088/1‑139 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                                                                     >  2:40918/1‑124 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGgcg                                                                     <  1:40918/124‑1 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCt                                                      >  1:94341/1‑139 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCt                                                      >  1:1294770/1‑139 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCt                                                      <  1:118023/139‑1 (MQ=255)
                                                                          tttCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCATTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCTACACTTTGGCATGTACTCATTGGCGTGGCGAAAACCACCt                                                      <  1:1007059/139‑1 (MQ=255)
                                                                            tCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCa                                                    >  2:454205/1‑139 (MQ=255)
                                                                             cGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAg                                                   <  2:94341/139‑1 (MQ=255)
                                                                             cGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAg                                                   <  1:1497420/139‑1 (MQ=255)
                                                                             cGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAg                                                   <  1:949310/139‑1 (MQ=255)
                                                                              gCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGt                                                  <  2:1272029/139‑1 (MQ=255)
                                                                               cTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGc                                                                                                                             >  1:554580/1‑63 (MQ=255)
                                                                                 ccGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               <  2:873903/139‑1 (MQ=255)
                                                                                 ccGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               <  1:671521/139‑1 (MQ=255)
                                                                                 ccGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               <  2:683408/139‑1 (MQ=255)
                                                                                   ggCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTa                                             >  1:1525802/1‑139 (MQ=255)
                                                                                         tttACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTc                                       >  1:1497685/1‑139 (MQ=255)
                                                                                         tttACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                                                                                    <  1:599533/94‑1 (MQ=255)
                                                                                         tttACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGc                                                                                    >  2:599533/1‑94 (MQ=255)
                                                                                                  aCCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCt                              <  1:746525/139‑1 (MQ=255)
                                                                                                  aCCGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCt                              <  2:117367/139‑1 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           <  1:566041/45‑1 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCca                                                                                                                           >  2:566041/1‑45 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAg                                                   >  2:1384363/1‑117 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCca                                                                                                                           >  1:140814/1‑45 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGcc                                                                                              <  2:765844/74‑1 (MQ=255)
                                                                                                   ccGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAac                                                           <  2:517573/109‑1 (MQ=255)
                                                                                                        gCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGcac                        >  2:630477/1‑139 (MQ=255)
                                                                                                        gCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGcac                        >  1:97496/1‑139 (MQ=255)
                                                                                                        gCCGGGGCCGTTGCTGCATTCTGTGCGCTGTTTGTCGCca                                                                                                                           >  1:1035663/1‑40 (MQ=255)
                                                                                                            gggCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGt                    >  1:823016/1‑139 (MQ=255)
                                                                                                            gggCCGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCCCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGt                    <  1:1387088/139‑1 (MQ=255)
                                                                                                             ggCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               >  2:345302/1‑111 (MQ=255)
                                                                                                             ggCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               <  1:345302/111‑1 (MQ=255)
                                                                                                              gCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGttt                  <  2:1447769/139‑1 (MQ=255)
                                                                                                               ccGTTGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTc                 <  2:1294770/139‑1 (MQ=255)
                                                                                                                  ttGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTAt                                                                                                                    <  2:1391402/37‑1 (MQ=255)
                                                                                                                  ttGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTAt                                                                                                                    >  1:1391402/1‑37 (MQ=255)
                                                                                                                  ttGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCgcgg                                                                  <  1:264776/87‑1 (MQ=255)
                                                                                                                  ttGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               >  1:929552/1‑106 (MQ=255)
                                                                                                                  ttGCTGCTTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGt                                               <  2:929552/106‑1 (MQ=255)
                                                                                                                    gctgctTTCTGTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCt            <  1:138599/139‑1 (MQ=255)
                                                                                                                           tCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGcc             >  1:1179942/1‑131 (MQ=255)
                                                                                                                           tCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGAt     <  1:777011/139‑1 (MQ=255)
                                                                                                                             tATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGATTa   >  2:295009/1‑139 (MQ=255)
                                                                                                                              aTGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGATTAc  <  1:1609231/139‑1 (MQ=255)
                                                                                                                              aTGCGCTGTTTGTCGCCACAGCTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGATTAc  <  2:1321032/139‑1 (MQ=255)
                                                                                                                              |                                                                                                                                          
CGATGCAGGAAATCTGGCACTCTTTTGTCTCCGGTATCTGGGCGCTGTTTCTTCCAGTCATCATTATTGGCGGGTTTCGCTCCGGCCTGTTTACCCCTACCGAAGCCGGGGCCGTTGCTGCTTTCTATGCGCTGTTTGTCGCCACAGTTATTTACCGTGAAATGACCTTCGCCACACTTTGGCATGTACTCATTGGCGCGGCGAAAACCACCTCAGTGGTTATGTTTCTGGTGGCCTCGGCACAAGTTTCCGCCTGGCTGATTAC  >  NC_000913/3745417‑3745681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑