Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913329,8390AG66.3% 103.0 / 65.2 83E126G (GAA→GGA) betTcholine transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (14/14);  new base G (27/28);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGC  >  NC_000913/329706‑329973
                                                                                                                                     |                                                                                                                                      
cTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGgcg                                                                                                                                   <  1:65811/139‑1 (MQ=255)
  cGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGcgcg                                                                                                                                 <  1:1481541/139‑1 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACa                                                                                                                            <  1:570653/131‑1 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACa                                                                                                                            >  2:570653/1‑131 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTg                                                                                                                    >  2:947226/1‑139 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGAt                                                                                                                      >  2:95509/1‑137 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGAt                                                                                                                      <  1:95509/137‑1 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTg                                                                                                                    >  1:791464/1‑139 (MQ=255)
               tCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTAGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTg                                                                                                                    >  2:1169966/1‑139 (MQ=255)
                    aCCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGcc                                                                                                               >  1:1361011/1‑139 (MQ=255)
                         aaTTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCcgcgcg                                                                                                          <  1:524060/139‑1 (MQ=255)
                          aTTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGt                                                                                                         <  1:999338/139‑1 (MQ=255)
                                        gTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTg                                                                                           >  2:1524906/1‑139 (MQ=255)
                                            ggcggcGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACg                                                                                       <  1:221327/139‑1 (MQ=255)
                                             gcggcgATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGc                                                                                      <  1:1524906/139‑1 (MQ=255)
                                                     gCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCAc                                                                              <  1:649374/139‑1 (MQ=255)
                                                                    gATCGGTATCGACCTGATGTTCTTCTCCGTAGTCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGc                                                               <  1:24067/139‑1 (MQ=255)
                                                                    gATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGc                                                               <  1:1169966/139‑1 (MQ=255)
                                                                    gATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGc                                                               <  2:258808/139‑1 (MQ=255)
                                                                                 cTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATg                                                  <  1:1257610/139‑1 (MQ=255)
                                                                                   gATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATgcg                                                <  1:947226/139‑1 (MQ=255)
                                                                                    aTGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATgcgc                                               >  1:468286/1‑139 (MQ=255)
                                                                                    aTGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATg                                                  >  1:1490823/1‑136 (MQ=255)
                                                                                    aTGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATg                                                  <  2:1490823/136‑1 (MQ=255)
                                                                                    aTGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACt                                                                             <  1:848708/109‑1 (MQ=255)
                                                                                    aTGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACt                                                                             >  2:848708/1‑109 (MQ=255)
                                                                                           tctcCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATggg                                        >  2:1154722/1‑139 (MQ=255)
                                                                                             tgcGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGCTTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCa                                      <  2:980952/137‑1 (MQ=255)
                                                                                             tcCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACg                                                                                       >  1:575458/1‑90 (MQ=255)
                                                                                             tcCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACg                                                                                       <  2:575458/90‑1 (MQ=255)
                                                                                             tcCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCa                                      <  2:249969/139‑1 (MQ=255)
                                                                                              ccGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCAt                                     <  1:1144170/139‑1 (MQ=255)
                                                                                               cGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATg                                    >  2:695868/1‑139 (MQ=255)
                                                                                                 tAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAg                                                                                                                           >  2:1164747/1‑50 (MQ=255)
                                                                                                 tAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAg                                                                                                                           <  1:1164747/50‑1 (MQ=255)
                                                                                                    ccGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTg                                                             >  2:331921/1‑109 (MQ=255)
                                                                                                    ccGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTg                                                             <  1:331921/109‑1 (MQ=255)
                                                                                                    ccGAACCGGTAACGCAGTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCt                               >  2:526036/1‑139 (MQ=255)
                                                                                                    ccGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCg                                                                                                   >  2:813784/1‑71 (MQ=255)
                                                                                                    ccGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCg                                                                                                   <  1:813784/71‑1 (MQ=255)
                                                                                                        aCCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                           <  2:1361011/139‑1 (MQ=255)
                                                                                                            gTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACt                       >  2:421479/1‑139 (MQ=255)
                                                                                                              aaCGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACttt                     >  1:1478266/1‑139 (MQ=255)
                                                                                                                 gCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGc                  <  2:506621/139‑1 (MQ=255)
                                                                                                                  cAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCt                 <  2:524073/139‑1 (MQ=255)
                                                                                                                    gTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTc                                                                                             <  1:491798/61‑1 (MQ=255)
                                                                                                                    gTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTc                                                                                             >  2:491798/1‑61 (MQ=255)
                                                                                                                    gTATATGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTAt               >  1:537162/1‑139 (MQ=255)
                                                                                                                      atatGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCgg                                                                >  1:518455/1‑88 (MQ=255)
                                                                                                                      atatGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCgg                                                                <  2:518455/88‑1 (MQ=255)
                                                                                                                      atatGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCg             <  1:526036/139‑1 (MQ=255)
                                                                                                                         tGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                           >  2:1214191/1‑122 (MQ=255)
                                                                                                                         tGCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                           <  1:1214191/122‑1 (MQ=255)
                                                                                                                          gCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGc                                                                                      <  2:726714/62‑1 (MQ=255)
                                                                                                                          gCAGCCGCCGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGc                                                                                      >  1:726714/1‑62 (MQ=255)
                                                                                                                            aGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATaa       >  1:1216430/1‑139 (MQ=255)
                                                                                                                             gccgccGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCgg                                                                >  2:27512/1‑81 (MQ=255)
                                                                                                                             gccgccGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCgg                                                                <  1:27512/81‑1 (MQ=255)
                                                                                                                             gccgccGGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAt      >  1:526024/1‑139 (MQ=255)
                                                                                                                             gccgccGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTa                <  2:136840/129‑1 (MQ=255)
                                                                                                                             gccgccGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTa                >  1:136840/1‑129 (MQ=255)
                                                                                                                              ccgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCgg                            <  2:777854/116‑1 (MQ=255)
                                                                                                                              ccgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCgg                            >  1:777854/1‑116 (MQ=255)
                                                                                                                              ccgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCt                 >  1:1043924/1‑127 (MQ=255)
                                                                                                                              ccgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCt                 <  2:1043924/127‑1 (MQ=255)
                                                                                                                               cgctggAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAttt    >  2:807029/5‑139 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGAt                                                                                                 <  1:345761/46‑1 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGAt                                                                                                 >  2:345761/1‑46 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTTACTACGGCTTAACCg                                                                 <  1:1009318/78‑1 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTTACTACGGCTTAACCg                                                                 >  2:1009318/1‑78 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGa                                                        <  2:35918/87‑1 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGa                                                        >  1:35918/1‑87 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGgcg                                 <  2:73462/110‑1 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGgcg                                 >  1:73462/1‑110 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                           >  2:17685/1‑116 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                           <  1:17685/116‑1 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAttt    >  2:507040/1‑139 (MQ=255)
                                                                                                                               cgccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAttt    >  2:188361/1‑139 (MQ=255)
                                                                                                                               cgccgGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCg                                                                                                   >  2:232290/1‑44 (MQ=255)
                                                                                                                               cgccgGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCg                                                                                                   <  1:232290/44‑1 (MQ=255)
                                                                                                                                 ccgGGAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGc  >  2:1357346/1‑139 (MQ=255)
                                                                                                                                 ccgGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTc                              <  2:652751/111‑1 (MQ=255)
                                                                                                                                 ccgGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTc                              >  1:652751/1‑111 (MQ=255)
                                                                                                                                     |                                                                                                                                      
CTCGGGCCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGC  >  NC_000913/329706‑329973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑