Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	50,162	C→T	H114Y (CAT→TAT)	folA →	dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	50,162	0	C	T	100.0%	180.3 / NA	56	H114Y (CAT→TAT)	folA	dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

ATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGC  >  NC_000913/50031‑50297                                                                                                                                    |                                                                                                                                        aTCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGa                                                                                                                                  >  2:1605617/1‑139 (MQ=255) aTCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGa                                                                                                                                  >  1:1196282/1‑139 (MQ=255)            tGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTgg                                                                                                                       >  1:860378/1‑139 (MQ=255)             ggatGAAGTCGGTCGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGACCAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGa                                                                                                                      <  2:1095252/136‑1 (MQ=255)              ggTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGaa                                                                                                                     >  2:699996/1‑139 (MQ=255)               gTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAg                                                                                                                    >  2:624571/1‑139 (MQ=255)                    gTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                               <  1:1572818/139‑1 (MQ=255)                       ggTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACAcc                                                                                                            >  1:1556243/1‑139 (MQ=255)                               aaGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTccc                                                                                                    <  2:860378/139‑1 (MQ=255)                                   cATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAGACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGAt                                                                                                >  1:882946/1‑139 (MQ=255)                                      cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGTGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTAc                                                                                             >  2:1228304/1‑139 (MQ=255)                                      cgcgGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTAc                                                                                             >  1:621943/1‑139 (MQ=255)                                                    aCGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTg                                                                               <  2:621943/139‑1 (MQ=255)                                                      gTACCAGAAATCATGGTGATTGGTGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTggg                                                                             <  1:1228304/139‑1 (MQ=255)                                                       tACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                                <  2:1309531/87‑1 (MQ=255)                                                       tACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                                >  1:1309531/1‑87 (MQ=255)                                                       tACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGa                                                                            >  1:493398/1‑139 (MQ=255)                                                         ccAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAAt                                                                          >  1:12087/1‑139 (MQ=255)                                                            gAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTg                                                                                                                        >  1:106465/1‑90 (MQ=255)                                                            gAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTg                                                                                                                        <  2:106465/90‑1 (MQ=255)                                                                   tGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCg                                                                                                                 >  2:307616/1‑90 (MQ=255)                                                                   tGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCg                                                                                                                 <  1:307616/90‑1 (MQ=255)                                                                   tGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTa                                                                                              >  1:358275/1‑109 (MQ=255)                                                                   tGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTa                                                                                              <  2:358275/109‑1 (MQ=255)                                                                        aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACt                                                                                >  2:95102/1‑118 (MQ=255)                                                                        aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACt                                                                                <  1:95102/118‑1 (MQ=255)                                                                        aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCa                                                                 <  1:1260525/133‑1 (MQ=255)                                                                        aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCa                                                                 >  2:1260525/1‑133 (MQ=255)                                                                        aTTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAAt                                                           >  1:1162765/1‑139 (MQ=255)                                                                            gcggcgGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                       <  1:1605617/139‑1 (MQ=255)                                                                               gcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                                >  1:1220215/1‑63 (MQ=255)                                                                               gcggTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACg                                                                                                                                <  2:1220215/63‑1 (MQ=255)                                                                                      cgTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGc                                                               >  1:1077375/1‑121 (MQ=255)                                                                                      cgTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGc                                                               <  2:1077375/121‑1 (MQ=255)                                                                                      cgTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGAt                                             >  2:1535926/1‑139 (MQ=255)                                                                                         ttATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGCCGCAGAAGTCGAAGGCGACACCCAGTTCCCGGATTACGAGCCGGATGACTGGGAATCAGTCTTCAGCGAATTCCACGATGCTGATgcg                                          <  2:109977/139‑1 (MQ=255)                                                                                         ttATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcg                                          <  1:699996/139‑1 (MQ=255)                                                                                          tATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcgc                                         >  2:873372/1‑139 (MQ=255)                                                                                            tGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAg                                       <  2:836492/139‑1 (MQ=255)                                                                                             gAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGTCACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGa                                      <  2:465288/139‑1 (MQ=255)                                                                                                   ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                               <  2:31003/60‑1 (MQ=255)                                                                                                   ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGac                                                                                                               >  1:31003/1‑60 (MQ=255)                                                                                                   ttCTTGCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACtctc                                >  1:292118/1‑139 (MQ=255)                                                                                                        gCCAAAAGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGc                           >  1:991279/1‑139 (MQ=255)                                                                                                          cAAAAGCGCAAAAACTGTATCTGACATATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTa                         <  1:1526213/139‑1 (MQ=255)                                                                                                             aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                       <  1:593529/106‑1 (MQ=255)                                                                                                             aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCa                                                       >  2:593529/1‑106 (MQ=255)                                                                                                             aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcg                                          >  2:1557194/1‑119 (MQ=255)                                                                                                             aaGCGCAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATgcg                                          <  1:1557194/119‑1 (MQ=255)                                                                                                                cgcAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCtt                   <  2:1162765/139‑1 (MQ=255)                                                                                                                  cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAAct                                   <  2:1433677/121‑1 (MQ=255)                                                                                                                  cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAAct                                   >  1:1433677/1‑121 (MQ=255)                                                                                                                  cAAAAACTGTATCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTg                 <  1:873372/139‑1 (MQ=255)                                                                                                                             tCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGa                                                    >  2:1660345/1‑93 (MQ=255)                                                                                                                             tCTGACGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGa                                                    <  1:1660345/93‑1 (MQ=255)                                                                                                                                 aCGTATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAgcggc  >  2:823368/1‑139 (MQ=255)                                                                                                                                    |                                                                                                                                        ATCGCGTAACGTGGGTGAAGTCGGTGGATGAAGCCATCGCGGCGTGTGGTGACGTACCAGAAATCATGGTGATTGGCGGCGGTCGCGTTTATGAACAGTTCTTGCCAAAAGCGCAAAAACTGTATCTGACGCATATCGACGCAGAAGTGGAAGGCGACACCCATTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGC  >  NC_000913/50031‑50297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑