Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 524,158 C→T L300L (CTG→TTG)  rhsD → Rhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913524,1580CT98.2% 175.5 / NA 57L300L (CTG→TTG) rhsDRhs protein with DUF4329 family putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (27/29);  total (28/29)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGACGC  >  NC_000913/524027‑524291
                                                                                                                                   |                                                                                                                                     
cGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATg                                                                                                                                  <  1:1559945/137‑1 (MQ=255)
cGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATg                                                                                                                                  >  2:1559945/1‑137 (MQ=255)
cGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                                >  1:1192704/1‑139 (MQ=255)
             cACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATAc                                                                                                                   >  1:1472080/1‑139 (MQ=255)
                  ctTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGga                                                                                                              >  1:1526547/1‑139 (MQ=255)
                           cttcttctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                                     <  2:253350/139‑1 (MQ=255)
                              cttctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGc                                                                                                  <  1:1384155/139‑1 (MQ=255)
                                tctGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                                <  2:1526547/139‑1 (MQ=255)
                                   gACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTgcgc                                                                                             <  2:1472080/139‑1 (MQ=255)
                                    aCAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGcc                                                                                            >  1:817048/1‑139 (MQ=255)
                                      aGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCAc                                                                                          >  2:823737/1‑139 (MQ=255)
                                        ttCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTg                                                                                        >  1:1398596/1‑139 (MQ=255)
                                           ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                     <  2:817048/139‑1 (MQ=255)
                                           ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                     <  1:1541248/139‑1 (MQ=255)
                                           ccGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                     <  1:823737/139‑1 (MQ=255)
                                                 tctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTac                                                                               <  2:231686/139‑1 (MQ=255)
                                                  ctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                                <  1:943085/121‑1 (MQ=255)
                                                  ctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTg                                                                                                >  2:943085/1‑121 (MQ=255)
                                                  ctctcAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTaca                                                                              >  2:1365859/1‑139 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCgg                                                                                                               >  1:204653/1‑95 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCgg                                                                                                               <  2:204653/95‑1 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTgg                                                                                       >  1:741309/1‑119 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                >  1:494575/1‑126 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                <  2:494575/126‑1 (MQ=255)
                                                             agcGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCGGCGCCACTgg                                                                                       <  2:741309/119‑1 (MQ=255)
                                                                      cGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGaa                                                          <  1:1365859/139‑1 (MQ=255)
                                                                         cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActg                                                       >  1:1003984/1‑139 (MQ=255)
                                                                         cacacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActg                                                       <  1:422574/139‑1 (MQ=255)
                                                                           cacTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAActgct                                                     >  2:402394/1‑139 (MQ=255)
                                                                             cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                <  1:507103/110‑1 (MQ=255)
                                                                             cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                >  2:507103/1‑110 (MQ=255)
                                                                             cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                   <  1:402394/139‑1 (MQ=255)
                                                                             cTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTgg                                                   <  2:1398596/139‑1 (MQ=255)
                                                                              tGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGc                                                                                                                       >  1:647713/1‑70 (MQ=255)
                                                                              tGCACGGTACCGAATACGGCCCCGACAGGGGTATACGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGc                                                                                                                       <  2:647713/70‑1 (MQ=255)
                                                                               gCCCGGTTCCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                                     <  2:1002891/87‑1 (MQ=255)
                                                                               gCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGccc                                                                                                    <  1:1631906/88‑1 (MQ=255)
                                                                               gCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGccc                                                                                                    >  2:1631906/1‑88 (MQ=255)
                                                                               gCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGcc                                                                                                     >  1:1002891/1‑87 (MQ=255)
                                                                                          aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATAcc                                                                                                                  >  2:1203481/1‑63 (MQ=255)
                                                                                          aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATAcc                                                                                                                  <  1:1203481/63‑1 (MQ=255)
                                                                                          aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                                      <  1:1007113/139‑1 (MQ=255)
                                                                                          aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                                      >  2:293725/1‑139 (MQ=255)
                                                                                          aaTACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCg                                      >  2:1013867/1‑139 (MQ=255)
                                                                                               ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCa                                                                                                                      >  2:743398/1‑54 (MQ=255)
                                                                                               ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCa                                                                                                                      <  1:743398/54‑1 (MQ=255)
                                                                                               ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                     >  1:331735/1‑87 (MQ=255)
                                                                                               ggCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTg                                                                                     <  2:331735/87‑1 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                                >  2:1559809/1‑42 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCa                                                                                                                                <  1:1559809/42‑1 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                <  1:1091435/90‑1 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTa                                                                                >  2:1091435/1‑90 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAAt                               >  1:1355399/1‑139 (MQ=255)
                                                                                                 ccccGACAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAAt                               <  1:1035987/139‑1 (MQ=255)
                                                                                                      aCAGGGGTATCCGCCTTTCGGCGGTGTGGTTGATGCACGACCCGGCATACCCGGAGAGCCTGACCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTAGATGACCGCAGCAATACGCa                          >  2:1081980/1‑139 (MQ=255)
                                                                                                         ggggTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGt                       >  1:624153/1‑139 (MQ=255)
                                                                                                                              tgtgGTTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGAcgc  <  2:624153/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CGGAAGAGGCCCGCACCTCTTCGCTATCTTCTTCTGACAGTTCCCGCCCTCTCTCAGCCTCAGCGTTCCCCGACACACTGCCCGGTACCGAATACGGCCCCGACAGGGGTATCCGCCTTTCGGCGGTGTGGCTGATGCACGACCCGGCATACCCGGAGAGCCTGCCCGCTGCGCCACTGGTGCGGTACACGTATACGGAAGCCGGTGAACTGCTGGCGGTATATGACCGCAGCAATACGCAGGTGCGCGCTTTCACGTATGACGC  >  NC_000913/524027‑524291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑