Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	649,648	A→G	G281G (GGT→GGC)	citE ←	citrate lyase, citryl‑ACP lyase (beta) subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	649,648	0	A	G	100.0%	106.2 / NA	31	G281G (GGT→GGC)	citE	citrate lyase, citryl‑ACP lyase (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

GCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAT  >  NC_000913/649525‑649782                                                                                                                            |                                                                                                                                       gCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTg                                                                                                                         <  1:1057551/139‑1 (MQ=255)     cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGt                                                                                                                     >  1:1171840/1‑139 (MQ=255)     cTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGt                                                                                                                     >  1:429721/1‑139 (MQ=255)         ccGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAg                                                                                                                 <  2:1171840/139‑1 (MQ=255)            ttCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGa                                                                                                              >  1:54893/1‑139 (MQ=255)                            tGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTg                                                                                                                         >  2:906811/1‑112 (MQ=255)                            tGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTg                                                                                                                         <  1:906811/112‑1 (MQ=255)                                                              tcttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGAcgc                                                            >  2:1632091/1‑139 (MQ=255)                                                                ttcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcgg                                                          <  1:1632091/139‑1 (MQ=255)                                                                 tcGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggc                                                         <  2:54893/139‑1 (MQ=255)                                                                  cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGCAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACgcggcg                                                        >  2:415321/1‑139 (MQ=255)                                                                  cgcgGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTACACAATCTTGCCGTTCAGGGCATCCACGCCGCGGCCTTCGCGAGCGGCGGCTTCAGCGGATTCTACGACgcggcg                                                        >  2:688601/1‑139 (MQ=255)                                                                       aTGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGt                                                   >  2:930700/1‑139 (MQ=255)                                                                                   tCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCActtctt                                       >  2:1514313/1‑139 (MQ=255)                                                                                    cTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttcttt                                      >  2:75663/1‑139 (MQ=255)                                                                                     tGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACttctttc                                     <  2:429721/139‑1 (MQ=255)                                                                                             agagCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCgg                             <  1:930700/139‑1 (MQ=255)                                                                                               agCACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTg                           >  1:1552571/1‑139 (MQ=255)                                                                                                 cACCAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCg                         >  2:409053/1‑139 (MQ=255)                                                                                                    cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTag                      <  2:1552571/139‑1 (MQ=255)                                                                                                    cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTag                      >  1:443337/1‑139 (MQ=255)                                                                                                    cAGACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTag                      >  1:178864/1‑139 (MQ=255)                                                                                                      gACGGGCGCGATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTagag                    >  2:887241/1‑139 (MQ=255)                                                                                                            cgcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTtgtg              <  1:75663/139‑1 (MQ=255)                                                                                                            cgcgATCGATAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTtgtg              <  1:887241/139‑1 (MQ=255)                                                                                                              cgatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTc                               >  1:832656/1‑120 (MQ=255)                                                                                                              cgatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTc                               <  2:832656/120‑1 (MQ=255)                                                                                                              cgatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgca            >  1:309744/1‑139 (MQ=255)                                                                                                              cgatcgatAACCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTgca            <  2:309744/139‑1 (MQ=255)                                                                                                                       aCCGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCaa   <  1:1514313/139‑1 (MQ=255)                                                                                                                        ccGGGCCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAt  <  1:1411150/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                       GCCGCTACCCGTTCTTGTCGTTGAGATTGTTCAATTTTCTGCGTCATTTTGATTGCCTTATTCTTCGCGGATGCCGGAAAGTTCTGCACGGGAGAGCACCAGACGGGCGCGATCGATAACCGGACCGTCCACCATCTTGCCGTTCAGGGAAACCACGCCGAGGCCTTCGCGAGCGGCGGCTTCAGCGGCTTCTACGACGCGGCGGGCGTGATCCACTTCTTTCTGGGTCGGTGCGTAGAGGTTGTGCAGCAGATCAAT  >  NC_000913/649525‑649782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑