Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 746,726 G→A *264* (TAG→TAA) 
A348A (GCC→GCT) 		 nei →
abrB ←		 endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase
regulator of aidB expression, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913746,7260GA100.0% 154.5 / NA 48*264* (TAG→TAA) 
A348A (GCC→GCT) 		nei
abrB		endonuclease VIII and 5‑formyluracil/5‑hydroxymethyluracil DNA glycosylase
regulator of aidB expression, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/25);  total (23/25)

ATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGC  >  NC_000913/746596‑746851
                                                                                                                                  |                                                                                                                             
atcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGTTGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCg                                                                                                                       <  2:1104127/139‑1 (MQ=255)
atcatGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCg                                                                                                                       <  2:1603299/139‑1 (MQ=255)
  catGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCt                                                                                                                     <  1:572327/139‑1 (MQ=255)
   atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCg                                                                                                                       <  2:221511/136‑1 (MQ=255)
   atGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCg                                                                                                                       >  1:221511/1‑136 (MQ=255)
         cgcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGgcgc                                                                                                              >  1:1139116/1‑139 (MQ=255)
          gcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGTCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                             <  2:988928/139‑1 (MQ=255)
          gcTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGTCGACCGCTTCGGCGCa                                                                                                             <  2:1620903/139‑1 (MQ=255)
            tGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATa                                                                                                           >  1:123488/1‑139 (MQ=255)
              tttCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                         <  1:254919/139‑1 (MQ=255)
                                   cGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATgg                                                                                    <  1:1210585/139‑1 (MQ=255)
                                          gCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                              <  1:225777/122‑1 (MQ=255)
                                          gCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAAc                                                                                              >  2:225777/1‑122 (MQ=255)
                                                 gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGc                                                                      >  1:26318/1‑139 (MQ=255)
                                                 gTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGc                                                                      >  1:739065/1‑139 (MQ=255)
                                                   gCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCaa                                                                    <  2:26318/139‑1 (MQ=255)
                                                    cGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                                    <  2:683404/90‑1 (MQ=255)
                                                    cGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                                    >  1:683404/1‑90 (MQ=255)
                                                            gtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                           <  2:123488/139‑1 (MQ=255)
                                                            gtgGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGaa                                                           <  1:600111/139‑1 (MQ=255)
                                                                 agcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGc                                                      >  2:559548/1‑139 (MQ=255)
                                                                 agcaTCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGc                                                      >  2:891955/1‑139 (MQ=255)
                                                                     tcatTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAg                                                  >  2:1637801/1‑139 (MQ=255)
                                                                       atTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                                        <  2:499579/99‑1 (MQ=255)
                                                                       atTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCa                                                                                        >  1:499579/1‑99 (MQ=255)
                                                                           agaAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTg                                            <  1:1637801/139‑1 (MQ=255)
                                                                                          cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                         <  1:91785/63‑1 (MQ=255)
                                                                                          cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                         >  2:91785/1‑63 (MQ=255)
                                                                                          cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                             <  1:891955/139‑1 (MQ=255)
                                                                                          cATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAg                             <  1:559548/139‑1 (MQ=255)
                                                                                                 cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                         >  1:1600510/1‑56 (MQ=255)
                                                                                                 cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAgg                                                                                                         <  2:1600510/56‑1 (MQ=255)
                                                                                                 cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTg                                            >  2:930791/1‑117 (MQ=255)
                                                                                                 cccGTTTTACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTg                                            <  1:930791/117‑1 (MQ=255)
                                                                                                     ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                                    <  2:1316299/41‑1 (MQ=255)
                                                                                                     ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCtt                                                                                                                    >  1:1316299/1‑41 (MQ=255)
                                                                                                     ttttACTGGTGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCgg                  >  1:1003118/1‑139 (MQ=255)
                                                                                                              tGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                     <  2:539454/127‑1 (MQ=255)
                                                                                                              tGCCCTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCatat                     >  1:539454/1‑127 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                             <  2:1468113/37‑1 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCa                                                                                                             >  1:1468113/1‑37 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCt           >  2:28588/1‑135 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCt           <  1:28588/135‑1 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCt       >  2:718350/1‑139 (MQ=255)
                                                                                                                cccTGGCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCACTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCt       >  2:966296/1‑139 (MQ=255)
                                                                                                                     gCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCAtt              >  2:105928/1‑127 (MQ=255)
                                                                                                                     gCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCAtt              <  1:105928/127‑1 (MQ=255)
                                                                                                                     gCTGCCAGCATTAAGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTgcggc  >  1:1175544/1‑139 (MQ=255)
                                                                                                                                  |                                                                                                                             
ATCATGGGGCGCTGTTTCGCTTTAAGGTTTTTCATCGAGATGGCGAACCGTGCGAACGTTGTGGCAGCATCATTGAGAAAACCACGCTGTCATCTCGCCCGTTTTACTGGTGCCCTGGCTGCCAGCACTAGGCCGACCGCTTCGGCGCATAGGTTGAAATAAACCGCGCAATGGCAGGCCCTGTCAGCAAAATACTGAACAGGCGTAGGGTTTGCATCGCCATAATGAGCGCCATATCGGCATTGCTCCCTGCGGC  >  NC_000913/746596‑746851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑