Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,972	C→T	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ← ybiH ←	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,972	0	C	T	100.0%	179.7 / NA	56	M1M (GTG→ATG) † *224* (TAG→TAA)	ybhG ybiH	putative membrane fusion protein (MFP) component of efflux pump, membrane anchor DUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/28);  total (28/28)

ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGG  >  NC_000913/829839‑830107                                                                                                                                      |                                                                                                                                        aCCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTCTTCATCATTACaa                                                                                                                                    >  1:126162/1‑139 (MQ=255)  ccGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaaa                                                                                                                                   <  1:1196279/139‑1 (MQ=255)  ccGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaaa                                                                                                                                   >  2:23114/1‑139 (MQ=255)  ccGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACaaa                                                                                                                                   <  1:652782/139‑1 (MQ=255)            atCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCg                                                                                                                         >  1:1395906/1‑139 (MQ=255)             tCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGa                                                                                                                        >  1:1109313/1‑139 (MQ=255)             tCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGa                                                                                                                        <  1:808079/139‑1 (MQ=255)                  gTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATc                                                                                                                   <  2:1109313/139‑1 (MQ=255)                   ttGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATcc                                                                                                                  >  2:1536089/1‑139 (MQ=255)                             gCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATc                                                                                                        <  1:1624208/139‑1 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                       >  1:878648/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCa                                                                                                       >  1:177958/1‑139 (MQ=255)                              cGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGATTTTTCATCATTACAAACTCCTTTGCGATAAGCCTTGCAAAAtaa                                                                                                       >  2:914123/1‑137 (MQ=255)                                tCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAgg                                                                                                     >  2:1258757/1‑139 (MQ=255)                                 cAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATTCTTGCAAAATCAGGt                                                                                                    <  2:1402050/139‑1 (MQ=255)                                 cAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGt                                                                                                    <  2:1091244/139‑1 (MQ=255)                                              tGGCGGCTTTGATACCACCAGTAGCCTCTGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  2:126162/139‑1 (MQ=255)                                              tGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  2:1395906/139‑1 (MQ=255)                                                gcggcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCa                                                                                     <  1:23114/139‑1 (MQ=255)                                                   gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                                  >  2:1286027/1‑139 (MQ=255)                                                   gcTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCg                                                                                  <  1:866673/139‑1 (MQ=255)                                                     tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGa                                                                                                                        >  1:766542/1‑99 (MQ=255)                                                     tttGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGa                                                                                                                        <  2:766542/99‑1 (MQ=255)                                                                caGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCt                                                                               >  1:913407/1‑129 (MQ=255)                                                                caGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCt                                                                               <  2:913407/129‑1 (MQ=255)                                                                  gTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGtt                                                                   >  2:728334/1‑139 (MQ=255)                                                                      ccTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGt                                                               >  1:1520449/1‑139 (MQ=255)                                                                           ggCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCtttt                                                          >  1:789869/1‑139 (MQ=255)                                                                             cAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTttct                                                        >  2:267021/1‑139 (MQ=255)                                                                               aCCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  1:1300873/91‑1 (MQ=255)                                                                               aCCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  2:1300873/1‑91 (MQ=255)                                                                                    ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                              >  1:661258/1‑62 (MQ=255)                                                                                    ggcggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCt                                                                                                                              <  2:661258/62‑1 (MQ=255)                                                                                      cggcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAc                                               <  1:92791/139‑1 (MQ=255)                                                                                        gcAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAAcgc                                             <  2:878648/139‑1 (MQ=255)                                                                                           aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATc                                                   <  1:1285717/130‑1 (MQ=255)                                                                                           aGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATc                                                   >  2:1285717/1‑130 (MQ=255)                                                                                             tACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTcc                                        <  1:65667/139‑1 (MQ=255)                                                                                             tACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTcc                                        <  1:728334/139‑1 (MQ=255)                                                                                                     ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       >  2:1524548/1‑84 (MQ=255)                                                                                                     ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGt                                                                                       <  1:1524548/84‑1 (MQ=255)                                                                                                     ccGCCAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTa                                >  2:1385670/1‑139 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACa                                                                                                                                     >  2:830371/1‑35 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  1:55376/1‑66 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  2:55376/66‑1 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                     >  1:1590041/1‑131 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGc                                     <  2:1590041/131‑1 (MQ=255)                                                                                                        ccAATCCGATCACGACAGGGTTTTTCATCATTACa                                                                                                                                     <  1:830371/35‑1 (MQ=255)                                                                                                          aaTCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  2:594991/1‑64 (MQ=255)                                                                                                          aaTCCGATCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  1:594991/64‑1 (MQ=255)                                                                                                                aTCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      <  2:1522744/58‑1 (MQ=255)                                                                                                                aTCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAg                                                                                                      >  1:1522744/1‑58 (MQ=255)                                                                                                                 tCACGACAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAAtt                    >  1:1574009/1‑139 (MQ=255)                                                                                                                       cAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTc               <  2:434315/138‑1 (MQ=255)                                                                                                                       cAGGTTTTTTCATCATTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTc               >  1:434315/1‑138 (MQ=255)                                                                                                                                   tcaTTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACgg  <  1:1385670/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        ACCGTACGAATATCCACGTTGCCATACAGCGTCAGGCCGTTATCCTGGCGGCTTTGATACCACCAGTAGCCTCCGGCAACCACGGCGGCAAGTACCACTACCGCCAATCCGATCACGACAGGTTTTTTCATCACTACAAACTCCTTTGCGATAATCCTTGCAAAATCAGGTCGATATGACAGGTCACCGTCTGGTTGATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGG  >  NC_000913/829839‑830107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑