Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	905,743	G→A	*277* (TAG→TAA)  R337R (CGC→CGT)	amiD → ybjS ←	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent, OM lipoprotein putative NAD(P)H‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	905,743	0	G	A	100.0%	210.1 / NA	65	*277* (TAG→TAA)  R337R (CGC→CGT)	amiD ybjS	1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase, Zn‑dependent, OM lipoprotein putative NAD(P)H‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/29);  total (36/29)

CACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGA  >  NC_000913/905613‑905879                                                                                                                                   |                                                                                                                                         cacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAg                                                                                                                                  <  2:66422/139‑1 (MQ=255)          agcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTg                                                                                                                         >  1:65341/1‑139 (MQ=255)          agcagcGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTg                                                                                                                         >  1:1481839/1‑139 (MQ=255)                                  tCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                                                                                 <  2:688509/139‑1 (MQ=255)                                       aTGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:109685/139‑1 (MQ=255)                                       aTGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:1137840/139‑1 (MQ=255)                                           aTTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTTGTCGCGTAGCCAGGCGGCAGTTTTTTCGTTAcc                                                                                        <  2:708809/139‑1 (MQ=255)                                            tttCCGCCCGACGTTATATAACGGCGAAGCGGTTGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                       >  2:359957/1‑139 (MQ=255)                                            tttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                       >  2:542891/1‑139 (MQ=255)                                            tttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCt                                                                                       >  1:585734/1‑139 (MQ=255)                                              tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                                                                                     >  2:1431272/1‑139 (MQ=255)                                               ccGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa                                                                                    >  2:419739/1‑139 (MQ=255)                                                  cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                                 >  2:127494/1‑139 (MQ=255)                                                   ccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCa                                                                                                                                   >  2:612940/1‑88 (MQ=255)                                                   ccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCa                                                                                                                                   <  1:612940/88‑1 (MQ=255)                                                               aaCGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCg                                                                    <  1:937944/139‑1 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAacg                                                                                                                                        >  1:1198783/1‑69 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                           >  1:1614481/1‑98 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                           <  2:1614481/98‑1 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  1:443457/113‑1 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            >  2:443457/1‑113 (MQ=255)                                                                 cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGACTAACCCGGCGGGTTGCCGTGGTTGCGTCGCCAGGCGGCAGTTGTCTCGATACCTTCTTCTAGGGtgatgaatggc                                                                  >  1:1085158/1‑134 (MQ=255)                                                                  ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCA‑GGTGATGACCGGCTg                                                                >  1:1498702/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGc                                                                                                               >  1:425153/1‑91 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGc                                                                                                               <  2:425153/91‑1 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  2:1194602/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  1:1045828/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  1:932770/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  1:1141849/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  1:541318/1‑139 (MQ=255)                                                                    cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               >  2:1631987/1‑139 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAAcgcg                                                                                                                                      <  2:361494/66‑1 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAAcgcg                                                                                                                                      >  1:361494/1‑66 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTc                                                                                                                             >  2:98590/1‑75 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTc                                                                                                                             <  1:98590/75‑1 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGt                                                                                                                          >  1:950319/1‑78 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGt                                                                                                                          <  2:548795/78‑1 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGt                                                                                                                          >  1:548795/1‑78 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGt                                                                                                                          <  2:950319/78‑1 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:546648/108‑1 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            >  1:546648/1‑108 (MQ=255)                                                                      aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATa                                                             >  2:1133229/1‑139 (MQ=255)                                                                            aTGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                       <  1:359957/139‑1 (MQ=255)                                                                              gCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                     <  1:1194602/139‑1 (MQ=255)                                                                                       cAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcg                                            <  2:721627/139‑1 (MQ=255)                                                                                        aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                                                                                 >  1:1272310/1‑85 (MQ=255)                                                                                        aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                                                                                 <  2:1272310/85‑1 (MQ=255)                                                                                        aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                                 <  2:1465130/101‑1 (MQ=255)                                                                                        aGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                                 >  1:1465130/1‑101 (MQ=255)                                                                                          gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  1:419739/139‑1 (MQ=255)                                                                                          gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  2:1141849/139‑1 (MQ=255)                                                                                            gATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAcgc                                       >  1:192973/1‑139 (MQ=255)                                                                                               tGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                    <  2:1481839/139‑1 (MQ=255)                                                                                                     aGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATcc                              <  1:542891/139‑1 (MQ=255)                                                                                                                 gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  1:1230552/65‑1 (MQ=255)                                                                                                                 gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            >  2:1230552/1‑65 (MQ=255)                                                                                                                 gaAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCA‑GGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                 >  2:1631212/1‑139 (MQ=255)                                                                                                                    aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         >  2:1083551/1‑113 (MQ=255)                                                                                                                    aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  1:1083551/113‑1 (MQ=255)                                                                                                                    aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaaa               >  1:1152387/1‑139 (MQ=255)                                                                                                                    aTACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaaaa               >  1:1087397/1‑139 (MQ=255)                                                                                                                      aCGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAAtt             <  2:65341/139‑1 (MQ=255)                                                                                                                          gCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                           <  2:37730/41‑1 (MQ=255)                                                                                                                          gCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAgg                                                                                                           >  1:37730/1‑41 (MQ=255)                                                                                                                            aGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGTCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACtt       >  2:1021722/1‑139 (MQ=255)                                                                                                                                ttAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCA‑GGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgaga  <  2:1498702/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                         CACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGA  >  NC_000913/905613‑905879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑