Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	77,159	C→T	M47I (ATG→ATA)	sgrR ←	transcriptional DNA‑binding transcriptional activator of sgrS sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	77,159	0	C	T	100.0%	170.1 / NA	53	M47I (ATG→ATA)	sgrR	transcriptional DNA‑binding transcriptional activator of sgrS sRNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/24);  total (29/24)

ATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGA  >  NC_000913/77031‑77293                                                                                                                                 |                                                                                                                                       aTACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCTCGTCAGCCAGCCGCGATCCTGTATGGTGTTGa                                                                                                                              <  2:983576/139‑1 (MQ=255) aTACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGa                                                                                                                              >  2:218378/1‑139 (MQ=255)           gCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTgcg                                                                                                                    <  1:486381/139‑1 (MQ=255)            cTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTgcgc                                                                                                                   >  2:204751/1‑139 (MQ=255)            cTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTgcgc                                                                                                                   >  1:130628/1‑139 (MQ=255)                 gcagGTCTTCCGCCCGCTGTTGCTGAAGCTCCAGCCCGGTATAGAAGAATGTAAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAgg                                                                                                                         >  2:958192/1‑128 (MQ=255)                 gcagGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAgg                                                                                                                         <  1:958192/128‑1 (MQ=255)                 gcagGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATg                                                                                                              >  2:1218518/1‑139 (MQ=255)                 gcagGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATg                                                                                                              >  2:596229/1‑139 (MQ=255)                 gcagGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATg                                                                                                              >  1:303558/1‑139 (MQ=255)                       cTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGacgacg                                                                                                        >  1:1536348/1‑139 (MQ=255)                         tCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGAcgcg                                                                                                      <  2:1442039/139‑1 (MQ=255)                           cgcccgcTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGag                                                                                                    <  2:1536348/139‑1 (MQ=255)                              ccgcTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGagcag                                                                                                 <  2:463666/139‑1 (MQ=255)                              ccgcTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGagcag                                                                                                 >  2:484572/1‑139 (MQ=255)                                               ccAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTg                                                                                                                      >  1:478956/1‑101 (MQ=255)                                               ccAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTg                                                                                                                      <  2:478956/101‑1 (MQ=255)                                               ccAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAg                                                                                >  2:1488560/1‑139 (MQ=255)                                               ccAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAg                                                                                >  2:1306886/1‑139 (MQ=255)                                                cAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGt                                                                               <  1:596229/139‑1 (MQ=255)                                                cAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGt                                                                               <  1:753903/139‑1 (MQ=255)                                                    ccGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTgc                                                                                                                     <  2:469013/97‑1 (MQ=255)                                                    ccGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTgc                                                                                                                     >  1:469013/1‑97 (MQ=255)                                                             agGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTtg                                                                  <  1:1306886/139‑1 (MQ=255)                                                             agGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTtg                                                                  >  2:694482/1‑139 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTg                                                                                    >  1:870987/1‑116 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTg                                                                                    <  2:870987/116‑1 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTcc                                                             >  2:1068969/1‑139 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTcc                                                             >  2:1431066/1‑139 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTcc                                                             >  2:1350892/1‑139 (MQ=255)                                                                  tGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTcc                                                             >  1:533306/1‑139 (MQ=255)                                                                       gACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGag                                                                                                    >  1:937926/1‑95 (MQ=255)                                                                       gACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGag                                                                                                    <  2:937926/95‑1 (MQ=255)                                                                       gACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGa                                                        >  2:51095/1‑139 (MQ=255)                                                                             aGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGag                                                                                                    <  1:1343267/89‑1 (MQ=255)                                                                             aGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGag                                                                                                    >  2:1343267/1‑89 (MQ=255)                                                                             aGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTAcc                                                  >  1:228716/1‑139 (MQ=255)                                                                                gTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTc                                               <  2:228716/139‑1 (MQ=255)                                                                                gTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTc                                               <  1:1488560/139‑1 (MQ=255)                                                                                       gcgcCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGAtt                                                      >  2:941388/1‑125 (MQ=255)                                                                                       gcgcCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGAtt                                                      <  1:941388/125‑1 (MQ=255)                                                                                          cccGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGc                                     >  1:523547/1‑139 (MQ=255)                                                                                            cGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCca                                   <  1:1218518/139‑1 (MQ=255)                                                                                              aCTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCcaca                                 >  1:512795/1‑139 (MQ=255)                                                                                                 tCCGCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGc                              <  1:1333546/139‑1 (MQ=255)                                                                                                    gCTTCCCACGTCAGCCAGCCGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGa                           >  1:884620/1‑139 (MQ=255)                                                                                                                agccagccGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAAt                                                                                           >  1:1103248/1‑63 (MQ=255)                                                                                                                agccagccGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAAt                                                                                           <  2:1103248/63‑1 (MQ=255)                                                                                                                 gccagccGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGttgttg              <  1:484572/139‑1 (MQ=255)                                                                                                                 gccagccGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGttgttg              <  1:1350892/139‑1 (MQ=255)                                                                                                                  ccagccGCGATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGTTGTTGc             >  2:185298/1‑139 (MQ=255)                                                                                                                          gATCCTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGc     <  1:218378/139‑1 (MQ=255)                                                                                                                             ccTGTATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGa  <  2:303558/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       ATACGATCCTGCTCCAGCAGGTCTTCCGCCCGCTGTTGCTGAAGCGCCAGCCCGGTATAGAGGAATGTCAGACGCGAGCGTTTACCGCGCCCGACTTCCGCTTCCCACGTCAGCCAGCCGCGATCCTGCATGGTGTTGAGCAGGGTGCGCATATGACGACGCGAGCAGCTCAATAACGCTGCCAGTTCGTTGAGCGTTGTGTCCTGCGATTTACCCTCGCAGCATTGCCACAGGCGGATGAACTGTTGTTGCAGACGAGCAGA  >  NC_000913/77031‑77293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑