Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	992,825	A→G	Y735C (TAC→TGC)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,825	0	A	G	100.0%	185.9 / NA	52	Y735C (TAC→TGC)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

GCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCAT  >  NC_000913/992707‑992948                                                                                                                       |                                                                                                                            gCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGa                                                                                                         <  1:1647060/139‑1 (MQ=255)  cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  1:1096503/139‑1 (MQ=255)  cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  1:1105838/139‑1 (MQ=255)  cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  1:644725/139‑1 (MQ=255)  cTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  1:929915/139‑1 (MQ=255)       gtgCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTc                                                                                                   <  1:850896/139‑1 (MQ=255)          cTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  2:396722/131‑1 (MQ=255)          cTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        >  1:396722/1‑131 (MQ=255)             gTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGa                                                                                             <  1:1121477/139‑1 (MQ=255)               gAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGa                                                                                             >  2:1177102/1‑137 (MQ=255)               gAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGa                                                                                             <  1:1177102/137‑1 (MQ=255)                    agcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                      >  2:212310/1‑139 (MQ=255)                    agcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                      <  2:1605182/139‑1 (MQ=255)                    agcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                      <  1:212310/139‑1 (MQ=255)                    agcaGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATaa                                                                                      >  1:304657/1‑139 (MQ=255)                        gTTCCACGAAGCAAACAATATGACTGATGTGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATgg                                                                                  >  1:1297621/1‑139 (MQ=255)                          tCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  1:43396/115‑1 (MQ=255)                          tCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        >  2:43396/1‑115 (MQ=255)                           ccACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGttt                                                                               >  1:454539/1‑139 (MQ=255)                                       cAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        >  1:1024130/1‑102 (MQ=255)                                       cAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  2:1024130/102‑1 (MQ=255)                                               cTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGccc                                                           >  1:133465/1‑139 (MQ=255)                                                tGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcg                                                          >  1:758424/1‑139 (MQ=255)                                                  aTGCGCTGGCGGTGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                        <  2:1010460/139‑1 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAg                                                                                                                   >  1:553121/1‑80 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAg                                                                                                                   <  2:553121/80‑1 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        >  1:186861/1‑91 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGaa                                                                                                        <  2:186861/91‑1 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGt                                                                                 <  2:1165339/114‑1 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGt                                                                                 >  1:1165339/1‑114 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                        >  1:1500264/1‑139 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                        >  2:566379/1‑139 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                        >  2:856121/1‑139 (MQ=255)                                                  aTGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCcggc                                                        <  1:566379/139‑1 (MQ=255)                                                     cgcTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCggcggc                                                     >  2:784587/1‑139 (MQ=255)                                                       cTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGa                                                   >  2:131758/1‑139 (MQ=255)                                                         ggcggcGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAAt                                                 <  2:1500264/139‑1 (MQ=255)                                                            ggcgCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGcc                                                            <  2:875792/125‑1 (MQ=255)                                                            ggcgCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGcc                                                            >  1:875792/1‑125 (MQ=255)                                                                               gCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGcc                           <  2:454539/139‑1 (MQ=255)                                                                                   cacaGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCa                                                                                                           >  2:1231074/1‑55 (MQ=255)                                                                                   cacaGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCa                                                                                                           <  1:1231074/55‑1 (MQ=255)                                                                                          gCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCAt                <  2:133465/139‑1 (MQ=255)                                                                                          gCCTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCAt                <  1:131758/139‑1 (MQ=255)                                                                                            cTTGCCGTGACGCGCTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCg              <  2:1297621/139‑1 (MQ=255)                                                                                                        cgcTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCAt  <  2:304657/139‑1 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTc                                                                                                   >  2:1169337/1‑40 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTc                                                                                                   <  1:1169337/40‑1 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTg                                                                                              >  2:17854/1‑45 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTg                                                                                              <  1:17854/45‑1 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCg              >  2:1621943/1‑125 (MQ=255)                                                                                                          cTGATGCAGGAGTGCGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCg              <  1:1621943/125‑1 (MQ=255)                                                                                                                       |                                                                                                                            GCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCAGGAGTACGACGACAAGTGGCATCAGAACGGTCTGGTGATGGATAAATGGTTTATCCTGCAAGCCACCAGCCCGGCGGCGAATGTGCTGGAGACGGTGCGCGGCCTGTTGCAGCATCGCTCATTTACCAT  >  NC_000913/992707‑992948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑