Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	111,433	G→A	*130* (TAG→TAA)	mutT →	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	111,433	0	G	A	100.0%	176.3 / NA	55	*130* (TAG→TAA)	mutT	dGTP‑preferring nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/29);  total (26/29)

GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTA  >  NC_000913/111302‑111548                                                                                                                                    |                                                                                                                    gTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                              >  1:513815/1‑139 (MQ=255)                   gAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                 <  1:131403/134‑1 (MQ=255)                   gAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgc                                                                                                 >  2:131403/1‑134 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgccg                                                                                               <  2:18883/123‑1 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTcgccg                                                                                               >  1:18883/1‑123 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGa                                                                                        >  1:1281374/1‑130 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGa                                                                                        <  2:1281374/130‑1 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGca                                                                                <  2:866752/138‑1 (MQ=255)                                aagaagGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGca                                                                                >  1:866752/1‑138 (MQ=255)                                     gggCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGAt                                                                          >  2:800652/1‑139 (MQ=255)                                       gCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                            >  1:1531386/1‑135 (MQ=255)                                       gCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACg                                                                            <  2:1531386/135‑1 (MQ=255)                                        cAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGcc                                                                       >  1:156805/1‑139 (MQ=255)                                          aCCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTg                                                                     >  2:950727/1‑139 (MQ=255)                                           cccGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGa                                                                    >  2:829970/1‑139 (MQ=255)                                            ccGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                              <  1:1613086/96‑1 (MQ=255)                                            ccGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGAt                                                                                                              >  2:1613086/1‑96 (MQ=255)                                             cGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATg                                                                  <  1:950727/139‑1 (MQ=255)                                               gTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCg                                                                >  1:1527309/1‑139 (MQ=255)                                                tGAGTGGATGTCGCTGGTCGGTCTTAGTGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGa                                                               >  2:909327/1‑139 (MQ=255)                                                  aGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACg                                                             <  1:829970/139‑1 (MQ=255)                                                      gATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                         >  2:628140/1‑139 (MQ=255)                                                      gATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                         <  2:709028/139‑1 (MQ=255)                                                      gATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTgg                                                         <  2:156805/139‑1 (MQ=255)                                                        tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGgcg                                                       <  1:1395384/139‑1 (MQ=255)                                                        tGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGgcg                                                       <  2:513815/139‑1 (MQ=255)                                                          tCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGcgcg                                                     <  1:596600/139‑1 (MQ=255)                                                            gCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTTTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTc                                                   >  2:965749/1‑139 (MQ=255)                                                            gCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTc                                                   >  1:671809/1‑139 (MQ=255)                                                                gtcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTAt                                               <  2:671809/139‑1 (MQ=255)                                                                gtcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTAt                                               <  1:1344252/139‑1 (MQ=255)                                                                 tcggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATc                                              <  1:628140/139‑1 (MQ=255)                                                                  cggtcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCa                                             <  2:1527309/139‑1 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                  <  2:662753/99‑1 (MQ=255)                                                                     tcTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCg                                                                                  >  1:662753/1‑99 (MQ=255)                                                                           tGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAg                                    <  1:965749/139‑1 (MQ=255)                                                                             ccGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  <  1:91623/139‑1 (MQ=255)                                                                             ccGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  <  1:1597981/139‑1 (MQ=255)                                                                               gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGtt                                                                                                      <  2:688766/69‑1 (MQ=255)                                                                               gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGtt                                                                                                      >  1:688766/1‑69 (MQ=255)                                                                               gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCGGACATTCGCACACGATGCCTGATg                                                                  <  2:1121604/105‑1 (MQ=255)                                                                               gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATg                                                                  >  1:1121604/1‑105 (MQ=255)                                                                               gatgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGATTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGAt                                >  1:1031570/1‑139 (MQ=255)                                                                                 tgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGgcg                                                       <  2:120102/114‑1 (MQ=255)                                                                                 tgatTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGgcg                                                       >  1:120102/1‑114 (MQ=255)                                                                                       tCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCACATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  <  2:453175/129‑1 (MQ=255)                                                                                       tCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCACATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  >  1:453175/1‑129 (MQ=255)                                                                                                           tAATTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTg    >  2:1558339/1‑139 (MQ=255)                                                                                                            aaTTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGt   <  2:1545683/139‑1 (MQ=255)                                                                                                            aaTTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGt   <  1:204449/139‑1 (MQ=255)                                                                                                             aTTGCGAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTa  >  2:1403413/1‑139 (MQ=255)                                                                                                                  gAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  <  2:753284/102‑1 (MQ=255)                                                                                                                  gAAGCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAggg                                  >  1:753284/1‑102 (MQ=255)                                                                                                                     gCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGa                                                               >  1:66655/1‑70 (MQ=255)                                                                                                                     gCTTAAACGTCTGTAAGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGa                                                               <  2:66655/70‑1 (MQ=255)                                                                                                                                    |                                                                                                                    GTCGAACGCTGGGAAGGGGAGCCGTGGGGTAAAGAAGGGCAACCCGGTGAGTGGATGTCGCTGGTCGGTCTTAATGCCGATGATTTTCCGCCAGCCAATGAACCGGTAATTGCGAAGCTTAAACGTCTGTAGGTCAGATAAGGCGTTTTCGCCGCATCCGACATTCGCACACGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTAAAGGGATTTCTAACTCATTGATAAATTTGTTTTTGTA  >  NC_000913/111302‑111548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑