Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	115,661	T→C	M22V (ATG→GTG)	hofC ←	assembly protein in type IV pilin biogenesis, transmembrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	115,661	0	T	C	100.0%	186.8 / NA	53	M22V (ATG→GTG)	hofC	assembly protein in type IV pilin biogenesis, transmembrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/21);  total (32/21)

TGAATGACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGC  >  NC_000913/115531‑115790                                                                                                                                   |                                                                                                                                  tgaatgaCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCtt                                                                                                                           >  1:975359/1‑139 (MQ=255)      gaCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCa                                                                                                                      >  2:609030/1‑139 (MQ=255)       aCTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCAt                                                                                                                     >  1:969239/1‑139 (MQ=255)          tCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGc                                                                                                                  <  2:975359/139‑1 (MQ=255)               gcTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCg                                                                                                             <  2:72913/139‑1 (MQ=255)                           tcGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgcca                                                                                                 >  2:917109/1‑139 (MQ=255)                             gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatg                                                                                               >  2:1228040/1‑139 (MQ=255)                             gCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatg                                                                                               >  1:613130/1‑139 (MQ=255)                                    cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                              >  2:541589/1‑133 (MQ=255)                                    cgcAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                              <  1:541589/133‑1 (MQ=255)                                              aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCt                                                                              >  1:798538/1‑139 (MQ=255)                                              aTGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCt                                                                              >  2:271968/1‑139 (MQ=255)                                                     ttCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGc                                                                       >  1:702212/1‑139 (MQ=255)                                                        gCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCCCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTTCTCGCCAt                                                                    >  1:984230/1‑139 (MQ=255)                                                            cAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCgg                                                                <  2:447814/139‑1 (MQ=255)                                                                  tAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATccc                                                          <  1:863419/139‑1 (MQ=255)                                                                         gTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcg                                                   <  2:1047469/139‑1 (MQ=255)                                                                          tAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTAcgc                                                  >  1:1192302/1‑139 (MQ=255)                                                                             ccATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCGTCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCAt                                                             >  1:225028/1‑125 (MQ=255)                                                                             ccATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCGTCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCAt                                                             <  2:225028/125‑1 (MQ=255)                                                                                tCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTa                                            <  2:1627214/139‑1 (MQ=255)                                                                                 cTGTTGCTGTAGTGCCCTAAGCAGTAAAGTACGGCTCGCTGCCCATAGCACCCCATCTTCCGCATTGCCGTAGCCGGGTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           >  2:745982/1‑139 (MQ=255)                                                                                 cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTATTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           <  2:984230/139‑1 (MQ=255)                                                                                 cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           >  1:474929/1‑139 (MQ=255)                                                                                 cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           >  2:1276687/1‑139 (MQ=255)                                                                                 cTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           <  1:271968/139‑1 (MQ=255)                                                                                   gTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           >  1:1510664/1‑137 (MQ=255)                                                                                   gTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           <  2:1510664/137‑1 (MQ=255)                                                                                           aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGt                                                                                                               <  1:13107/61‑1 (MQ=255)                                                                                           aGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGt                                                                                                               >  2:13107/1‑61 (MQ=255)                                                                                            gTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAgg                                >  1:520097/1‑139 (MQ=255)                                                                                              gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           <  1:1320508/126‑1 (MQ=255)                                                                                              gCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTaa                                           >  2:1320508/1‑126 (MQ=255)                                                                                               ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGTGCATTGCCGTCGCCGGTTatgccatgcc                                                                                             <  1:1026557/75‑1 (MQ=255)                                                                                               ccATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgcc                                                                                             >  2:1026557/1‑75 (MQ=255)                                                                                                 aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                              >  2:591591/1‑72 (MQ=255)                                                                                                 aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTatgccatgc                                                                                              <  1:591591/72‑1 (MQ=255)                                                                                                 aTAAGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                           <  2:702212/139‑1 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                 >  2:652621/1‑130 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAg                                 <  1:652621/130‑1 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                        >  2:990144/1‑139 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                        >  1:633587/1‑139 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                        >  1:491096/1‑139 (MQ=255)                                                                                                    aGCAGTAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAg                        >  1:1058548/1‑139 (MQ=255)                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                    >  1:891284/1‑90 (MQ=255)                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCAt                                                                    <  2:891284/90‑1 (MQ=255)                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                           >  2:1066475/1‑131 (MQ=255)                                                                                                         tAAAGTACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGtt                           <  1:1066475/131‑1 (MQ=255)                                                                                                              tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                               <  2:1460595/90‑1 (MQ=255)                                                                                                              tACGGCTCTCTGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGc                                                               >  1:1460595/1‑90 (MQ=255)                                                                                                                   ctctctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCCTGCTGCATCGCCAGTACGAGGATTAACTCTTAAAAGGTGGTTAAGCCTTGCTCTACGGcc         >  2:570171/1‑139 (MQ=255)                                                                                                                      tctGCCCATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAgg      >  1:597817/1‑139 (MQ=255)                                                                                                                          cccATAGCACCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGc  <  2:1192302/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  TGAATGACTTCCGCGCTTTTATCTCCTCGCCACTGCGCAGAATTGATGGCGATTCGCTTCAGGCTTAGTGGGGTAACCATCTGTTGCTGTAGTGCCATAAGCAGTAAAGTACGGCTCTCTGCCCATAGCATCCCATCTTGCGCATTGCCGTCGCCGGTTATGCCATGCCAGCGCCAGAGTTGCTTACTCGCCATGCGGCATCCCCAGTACGCGGATTAACTCTTCAAAGGTGGTTAAGCCTTGCTCTACGGCCAGGCAGC  >  NC_000913/115531‑115790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑