| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,266,086 | C→G | Q359E (CAG→GAG) | prfA → | peptide chain release factor RF‑1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,266,086 | 0 | C | G | 100.0% | 96.3 / NA | 29 | Q359E (CAG→GAG) | prfA | peptide chain release factor RF‑1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (11/18); total (11/18) | |||||||||||
GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218 | taaccgcttgatccatgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtggaggag < 1:1078024‑M2/6‑1 (MQ=255) atccatgcgccaacgaaatcacttcaacaggccgcccgtgaaggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGa < 1:602746‑M2/16‑1 (MQ=255) catgcgccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAata < 1:51111‑M2/19‑1 (MQ=255) gccaacgaaatcacttcaacaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAac < 1:1146022‑M2/24‑1 (MQ=255) acaggccgcccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGTAATAATGGAATATCAACACTGGTTACGTGAAGCaa < 2:1266097‑M2/42‑1 (MQ=255) cgcccgtgacggggataacgatcgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatgattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGcc < 2:966146‑M2/48‑1 (MQ=255) cccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCaa < 2:1327203‑M2/42‑1 (MQ=255) cccgtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCaa > 1:1327203‑M2/90‑131 (MQ=255) gtgacggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACtt < 2:236777‑M2/53‑1 (MQ=255) acggggataacgaacgcctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAAATTCAg > 2:1111412‑M2/84‑139 (MQ=255) ctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcgcg < 2:1118069‑M2/73‑1 (MQ=255) ctgaatattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCcgcg < 2:816057‑M2/73‑1 (MQ=255) atattctgcgcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGt < 1:825021‑M2/77‑1 (MQ=255) gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATc > 2:678358‑M2/54‑74 (MQ=255) gcgacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATc < 1:678358‑M2/21‑1 (MQ=255) gacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa > 2:447557‑M2/52‑138 (MQ=255) gacagcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGaaa < 1:447557‑M2/87‑1 (MQ=255) gcctcgggctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCctg < 2:122423‑M2/92‑1 (MQ=255) gctggagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGc < 2:1594878‑M2/99‑1 (MQ=255) gagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt < 1:409893‑M2/103‑1 (MQ=255) gagtaacagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGt > 2:409893‑M2/37‑139 (MQ=255) cagtacatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCgg > 1:1546032‑M2/31‑139 (MQ=255) acatcattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACAATGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCaga > 2:1019984‑M2/27‑139 (MQ=255) cattttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGc > 1:1176264‑M2/23‑139 (MQ=255) attttctttttttacagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCg < 1:1647848‑M2/118‑1 (MQ=255) taaaaaaagagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg > 1:39115‑M2/16‑91 (MQ=255) taaaaaaagagggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCgcggcg < 2:39115‑M2/76‑1 (MQ=255) gggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTgg > 2:691643‑M2/6‑101 (MQ=255) gggtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTgg < 1:691643‑M2/96‑1 (MQ=255) gtgGAGGAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCtt > 1:1146664‑M2/4‑139 (MQ=255) | GCAGGGGCGCGTTACCGATCACCGCATCAACCTGACGCTCTACCGCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCGGCGTTGTCCGAGCAGGAATAATGGAATATCAACACTGGTTACGTGAAGCAATAAGCCAACTTCAGGCGAGCGAAAGCCCGCGGCGTGATGCTGAAATCCTGCTGGAGCATGTTACCGGCAGAGGGCGTACTTTTATTCTCGCCTT > NC_000913/1265950‑1266218 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |