Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,385,339	C→T	P408L (CCG→CTG)	ycjX →	DUF463 family protein, puatative P‑loop NTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,385,339	0	C	T	100.0%	97.5 / NA	31	P408L (CCG→CTG)	ycjX	DUF463 family protein, puatative P‑loop NTPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/16);  total (15/16)

AAAATGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATT  >  NC_000913/1385204‑1385466                                                                                                                                        |                                                                                                                                aaaaTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  1:1264080/139‑1 (MQ=255) aaaaTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  2:1157795/139‑1 (MQ=255)    aTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTc                                                                                                                             >  2:240449/1‑137 (MQ=255)    aTGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTc                                                                                                                             <  1:240449/137‑1 (MQ=255)           gTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTAt                                                                                                                    >  1:156769/1‑139 (MQ=255)            ttCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATc                                                                                                                   <  2:1504412/139‑1 (MQ=255)                              gACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCAc                                                                                                 >  2:616613/1‑139 (MQ=255)                                         gCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGt                                                                                      <  2:74445/139‑1 (MQ=255)                                            ggCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAg                                                                                   <  1:616613/139‑1 (MQ=255)                                              cGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGc                                                                                 <  2:156769/139‑1 (MQ=255)                                                 cAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGtt                                                                              <  1:358535/139‑1 (MQ=255)                                                                                 gTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCAt                                              >  1:816610/1‑139 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              >  2:1442119/1‑56 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCt                                                                                                                              <  1:1442119/56‑1 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCg                                                                                                    >  1:1552143/1‑82 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCg                                                                                                    <  2:1552143/82‑1 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTc                                           >  1:1367687/1‑139 (MQ=255)                                                                                    aaCGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTc                                           >  2:61178/1‑139 (MQ=255)                                                                                      cGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGt                                         >  2:1350230/1‑139 (MQ=255)                                                                                          gagaAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGc                                     >  1:1140521/1‑139 (MQ=255)                                                                                                       cgcTGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGt                        >  2:1512236/1‑139 (MQ=255)                                                                                                          tGCGTGGTAATCGACTTAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCACTTCGTCCGCAGGTGATGGATGTCGa                     <  2:1140521/139‑1 (MQ=255)                                                                                                                         ttAGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCa      <  2:1315374/139‑1 (MQ=255)                                                                                                                          tAGCGATGGTGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatggatg                         <  1:1490050/119‑1 (MQ=255)                                                                                                                          tAGCGATGGTGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTgatggatg                         >  2:1490050/1‑119 (MQ=255)                                                                                                                           aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGa                     >  1:601492/1‑122 (MQ=255)                                                                                                                           aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGa                     <  2:601492/122‑1 (MQ=255)                                                                                                                           aGCGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCata    >  1:749347/1‑139 (MQ=255)                                                                                                                             cGATGGCGCACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATAtt  <  1:1512236/139‑1 (MQ=255)                                                                                                                                  gcgcACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACt            <  1:363640/124‑1 (MQ=255)                                                                                                                                  gcgcACTGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACt            >  2:363640/1‑124 (MQ=255)                                                                                                                                        |                                                                                                                                AAAATGCGGCGTTCGAAGGGATCAGCATGGACTGCCTGGGGCTGGCGTCAGTTCAGGCGACCACCAGCGGCATTATTGATGTTAACGGTGAGAAAATCCCGGCGCTGCGTGGTAATCGACTTAGCGATGGCGCACCGCTCACTGTTTATCCTGGCGAAGTTCCCGCACGTTTGCCTGGTCAGGCGTTCTGGGATAAGCAAGGCTTCCAGTTTGAGGCATTTCGTCCGCAGGTGATGGATGTCGACAAACCACTACCGCATATT  >  NC_000913/1385204‑1385466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑