| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,416,884 | A→G | V168A (GTT→GCT) | recE ← | Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,416,884 | 0 | A | G | 96.5% | 190.4 / ‑2.7 | 57 | V168A (GTT→GCT) | recE | Rac prophage, exonuclease VIII, 5' to 3' specific dsDNA exonuclease |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (29/26); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
CTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAG > NC_000913/1416756‑1417019 | cTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGc > 1:576547/1‑139 (MQ=255) aaaaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTaa > 2:401177/1‑139 (MQ=255) aaaaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTAGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGAGGGAAACAGCCAGGGCAGTaa > 2:1169447/1‑139 (MQ=255) aaaaGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACa < 1:1607924/139‑1 (MQ=255) aGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGcc < 2:1329842/139‑1 (MQ=255) ttATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAg > 1:503675/1‑139 (MQ=255) cAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttct < 2:806472/139‑1 (MQ=255) cAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttct < 1:401177/139‑1 (MQ=255) cAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGcc > 1:1235410/1‑139 (MQ=255) ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGccc < 2:1050364/138‑1 (MQ=255) ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCg < 2:1080961/139‑1 (MQ=255) ggTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTAGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGccc > 1:1050364/1‑138 (MQ=255) ggTTTGAATTACCAGCATTAGGGAAAACTTTGTCAGTGTCACGAACCGGTTTGTGGTGTTCGCGAATTTCTAGCGGGTCGAGCAGGCTGGCTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCGTGCGCCCg > 2:1602848/1‑139 (MQ=255) gTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGa < 1:1169447/139‑1 (MQ=255) gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc > 2:842383/1‑101 (MQ=255) gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc < 1:842383/101‑1 (MQ=255) gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc > 1:1455859/1‑128 (MQ=255) gAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc < 2:1455859/128‑1 (MQ=255) ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGcc > 2:6791/1‑96 (MQ=255) ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGcc < 1:6791/96‑1 (MQ=255) ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATgtgt > 2:716606/1‑138 (MQ=255) ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATgtgt < 1:716606/138‑1 (MQ=255) ccAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTa > 2:1437342/1‑139 (MQ=255) aGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTgg > 2:97205/1‑139 (MQ=255) ggAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCaa > 1:613079/1‑123 (MQ=255) ggAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCaa < 2:613079/123‑1 (MQ=255) gAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc < 1:767876/111‑1 (MQ=255) gAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGttcttc > 2:767876/1‑111 (MQ=255) aaCTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAggg < 1:1085011/86‑1 (MQ=255) aaCTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCg > 2:944453/1‑139 (MQ=255) aaCTTTGACAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAggg > 2:1085011/1‑86 (MQ=255) tGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTcgcg > 1:1564395/1‑139 (MQ=255) tGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTcgcg > 1:1130792/1‑139 (MQ=255) gTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc < 1:1355414/75‑1 (MQ=255) gTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAagcagc > 2:1355414/1‑75 (MQ=255) tgtCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAAc > 2:641678/1‑139 (MQ=255) tCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGt > 1:99013/1‑139 (MQ=255) gTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCgg > 2:293586/1‑139 (MQ=255) tgGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGttttt < 2:1532143/139‑1 (MQ=255) gAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCg < 1:1295898/139‑1 (MQ=255) aGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGc > 2:1078665/1‑139 (MQ=255) ttCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTg > 1:165966/1‑139 (MQ=255) ttCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTg < 2:1130792/139‑1 (MQ=255) tCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTcc < 1:1292561/104‑1 (MQ=255) tCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTcc > 2:1292561/1‑104 (MQ=255) ttCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGa < 1:97205/139‑1 (MQ=255) tCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAg < 1:641678/139‑1 (MQ=255) ccAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGc < 2:1564395/139‑1 (MQ=255) cAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCa < 2:165966/139‑1 (MQ=255) gggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGcc < 1:1437342/139‑1 (MQ=255) ggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCa > 1:526582/1‑139 (MQ=255) ggTCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCa > 1:345036/1‑139 (MQ=255) tCGAGCAGGCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAAc > 2:171010/1‑139 (MQ=255) ggCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATggg < 2:45650/139‑1 (MQ=255) ggCTGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATggg < 2:99013/139‑1 (MQ=255) tGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAg < 1:1322102/54‑1 (MQ=255) tGGTTTTGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAg > 2:1322102/1‑54 (MQ=255) ttttGTGGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTc < 2:345036/139‑1 (MQ=255) tgGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCgtg > 2:621315/1‑107 (MQ=255) tgGGAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCgtg < 1:621315/107‑1 (MQ=255) ggAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCa > 2:1398209/1‑139 (MQ=255) gAAGCAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAg < 2:1311142/139‑1 (MQ=255) | CTTCGAAAAAAGCAGTTATCAGTCCCAGGTTTGAATTACCAGGATTAGGGAAAACTTTGTCAGTGTCACGAACCAGTTTGTGGAGTTCGCGAATTTCCAGCGGGTCGAGCAGGCTGGTTTTGTGGGAAACAGCCAGGGCAGTAACAGCCGGTAGTTCTTCAGCCCGAGCAATGTGTAATGCCTGGAGTCCGTCGCGTGAAACGTGCGTTACCGGTTTTTCGCTGCCGTGTTGAGCAAGCCAACGAATGGGCAGTTCCTGGCCAG > NC_000913/1416756‑1417019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |