| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 125,227 | T→C | R737R (CGT→CGC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 125,227 | 0 | T | C | 100.0% | 220.3 / NA | 61 | R737R (CGT→CGC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamine triphosphate‑binding |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (32/29); total (32/29) | |||||||||||
CTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACGCTGGAACATGCTGCACCCGCTGGAA > NC_000913/125093‑125362 | cTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACg > 1:56276/1‑139 (MQ=255) tGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGt > 1:828131/1‑139 (MQ=255) aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc > 2:739434/1‑139 (MQ=255) aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc > 1:1480075/1‑139 (MQ=255) aaCGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTcc > 1:1602768/1‑139 (MQ=255) aaaaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGa < 2:2051/139‑1 (MQ=255) aaaCTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGaa > 1:349533/1‑139 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagcagc < 2:1480075/139‑1 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagcagc > 2:155199/1‑139 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagcagc > 1:360976/1‑139 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag > 2:276323/1‑135 (MQ=255) aCCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag < 1:276323/135‑1 (MQ=255) gCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc > 1:1468063/1‑139 (MQ=255) ccGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATcc > 2:1515894/1‑139 (MQ=255) gCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTgg < 2:1468063/139‑1 (MQ=255) aaTGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCg < 2:502560/139‑1 (MQ=255) tGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGaa > 1:810392/1‑139 (MQ=255) ggAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGAt > 2:999209/1‑139 (MQ=255) aGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt < 2:222637/139‑1 (MQ=255) aGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCt < 2:810392/139‑1 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTg > 1:1579930/1‑97 (MQ=255) ggTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTg < 2:1579930/97‑1 (MQ=255) ccGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCa < 2:15636/84‑1 (MQ=255) tAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTtat > 2:1624287/1‑139 (MQ=255) aaaGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTtata < 2:1163506/139‑1 (MQ=255) aaGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAg > 1:738114/1‑139 (MQ=255) aGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc > 2:175526/1‑100 (MQ=255) aGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATc < 1:175526/100‑1 (MQ=255) ggTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCAc > 2:1052964/1‑78 (MQ=255) ggTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCg < 1:739434/139‑1 (MQ=255) ggTATCTACAAACTAGAAACTATTGAAGGTGGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCAc < 1:1052964/78‑1 (MQ=255) tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGTTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg < 1:999209/139‑1 (MQ=255) tATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTg < 2:738114/139‑1 (MQ=255) aTCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGa < 2:1602768/139‑1 (MQ=255) tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag < 1:1051110/84‑1 (MQ=255) tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAag > 2:1051110/1‑84 (MQ=255) tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAc > 1:1158295/1‑139 (MQ=255) tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAc > 2:409894/1‑139 (MQ=255) tCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAc > 1:731646/1‑139 (MQ=255) tACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAcct > 1:876791/1‑139 (MQ=255) tACAAACTCGAAACTAATGAAGGAAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGAcct > 1:160846/1‑139 (MQ=255) cTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCa > 2:101317/1‑139 (MQ=255) cGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagca < 1:302333/76‑1 (MQ=255) cGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAagca > 2:302333/1‑76 (MQ=255) tATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTg < 1:155199/139‑1 (MQ=255) ggTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg < 1:409894/139‑1 (MQ=255) tAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGAt < 2:828131/139‑1 (MQ=255) cAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGt > 1:504059/1‑139 (MQ=255) gTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGa > 2:17304/1‑139 (MQ=255) tagaGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg < 1:27669/125‑1 (MQ=255) tagaGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTg > 2:27669/4‑128 (MQ=255) aGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTg < 2:1158055/118‑1 (MQ=255) aGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTg > 1:1158055/1‑118 (MQ=255) gTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATtgtg < 2:1477465/139‑1 (MQ=255) gTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATtgtg < 1:101317/139‑1 (MQ=255) aGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTAc > 1:888161/1‑68 (MQ=255) aGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTAc < 2:888161/68‑1 (MQ=255) aGCTGCTCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACg < 1:17304/139‑1 (MQ=255) tgctCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACGCTg < 2:504059/139‑1 (MQ=255) tCGGCTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACGCTGGaa < 1:1515894/139‑1 (MQ=255) cTCCGGTTCTATCCTGCGCCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACGCTGGAACAtg > 1:1461240/1‑139 (MQ=255) gcgcCACGTCCGTGAAGCAGCTGAGATCCGGGCGAAAAATTACGGCGTAGGTTCTGCCGTTTATAGGGTGACCTCCTTCACCGAGCTGGCCGGTAATGGT‑AAGAATGTGAAAGGCTGGAAAATGCTGCACCCCCTGaaa > 2:1194728/1‑139 (MQ=255) | CTGAACGAAAACTACCACATGCCGGCAATGCCGGAAGGTGCTGAGGAAGGTATCCGTAAAGGTATCTACAAACTCGAAACTATTGAAGGTAGCAAAGGTAAAGTTCAGCTGCTCGGCTCCGGTTCTATCCTGCGTCACGTCCGTGAAGCAGCTGAGATCCTGGCGAAAGATTACGGCGTAGGTTCTGACGTTTATAGCGTGACCTCCTTCACCGAGCTGGCGCGTGATGGTCAGGATTGTG‑AACGCTGGAACATGCTGCACCCGCTGGAA > NC_000913/125093‑125362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |