Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,459,052	G→A	*263* (TAG→TAA)	paaG →	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,459,052	0	G	A	100.0%	181.1 / NA	56	*263* (TAG→TAA)	paaG	1,2‑epoxyphenylacetyl‑CoA isomerase, oxepin‑CoA‑forming
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

CGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCA  >  NC_000913/1458917‑1459185                                                                                                                                        |                                                                                                                                      cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCCGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    >  2:372718/1‑139 (MQ=255) cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    <  2:1125236/139‑1 (MQ=255) cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    >  1:317530/1‑139 (MQ=255) cgcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    <  2:433759/139‑1 (MQ=255)  gcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    <  2:719061/138‑1 (MQ=255)  gcTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACat                                                                                                                                    >  1:719061/1‑138 (MQ=255)    tGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACatgat                                                                                                                                 >  1:842874/1‑139 (MQ=255)          cAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATaaa                                                                                                                           <  1:294197/139‑1 (MQ=255)           aaTACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAt                                                                                                                          >  1:680099/1‑139 (MQ=255)                   cGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAAc                                                                                                                  >  1:200074/1‑139 (MQ=255)                          cAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                           >  2:1554894/1‑139 (MQ=255)                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                       >  2:538458/1‑116 (MQ=255)                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgtg                                                                                                                       <  1:538458/116‑1 (MQ=255)                                     ggAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAg                                                                                                <  2:680099/139‑1 (MQ=255)                                       aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg                                                                                              >  2:1491886/1‑139 (MQ=255)                                       aaCGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCg                                                                                              >  1:1318251/1‑139 (MQ=255)                                          gTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                             >  2:477142/1‑137 (MQ=255)                                          gTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCgg                                                                                             <  1:477142/137‑1 (MQ=255)                                           tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                           >  2:769634/1‑122 (MQ=255)                                           tGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCa                                                                                                           <  1:769634/122‑1 (MQ=255)                                                 tCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                     <  2:80349/106‑1 (MQ=255)                                                 tCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                     >  1:80349/1‑106 (MQ=255)                                                    gCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgt                                                                                                                        >  2:1509359/1‑100 (MQ=255)                                                    gCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAAtgt                                                                                                                        <  1:1509359/100‑1 (MQ=255)                                                       aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                              <  2:1141970/139‑1 (MQ=255)                                                       aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                              <  2:1266238/139‑1 (MQ=255)                                                       aCTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                              <  1:1376136/139‑1 (MQ=255)                                                        cTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACAtgatga                                                                                                                               <  2:630576/89‑1 (MQ=255)                                                        cTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACAtgatga                                                                                                                               >  1:630576/1‑89 (MQ=255)                                                         ttGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcaggca                                                                            >  2:483654/1‑139 (MQ=255)                                                                   gaGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGt                                                                  <  2:200074/139‑1 (MQ=255)                                                                    aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                     <  2:295764/87‑1 (MQ=255)                                                                    aGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCa                                                                                                                     >  1:295764/1‑87 (MQ=255)                                                                      cgcAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgc                                                               >  1:644560/1‑139 (MQ=255)                                                                           aTTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCa                                                          <  1:372718/139‑1 (MQ=255)                                                                                     aGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                >  1:587440/1‑139 (MQ=255)                                                                                     aGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                >  1:1320364/1‑139 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATTACATGATGATAAATGTGCAAACTGTGGCAGTCATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgt                                                               <  1:1017891/120‑2 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTtgc                                                               >  2:1017891/1‑120 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAg                                            >  1:55584/1‑139 (MQ=255)                                                                                         gtCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGAGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAg                                            >  1:882405/1‑139 (MQ=255)                                                                                          tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                             <  1:1422679/73‑1 (MQ=255)                                                                                          tCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTgg                                                                                                             >  2:1422679/1‑73 (MQ=255)                                                                                               gCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTa                                      <  2:644560/139‑1 (MQ=255)                                                                                                  ttCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTg                                   >  1:966518/1‑139 (MQ=255)                                                                                                               gCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                >  2:738224/1‑113 (MQ=255)                                                                                                               gCTCACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGaca                                                <  1:738224/113‑1 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCaaa                                                                                                                   >  2:285197/1‑43 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCaaa                                                                                                                   <  1:285197/43‑1 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                              <  2:980367/80‑1 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGggcagg                                                                              >  1:980367/1‑80 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCa                                                          <  2:105849/100‑1 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCa                                                          >  1:105849/1‑100 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                   <  1:1554894/139‑1 (MQ=255)                                                                                                                  cACCGCAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTctgctg                   >  1:1312738/1‑139 (MQ=255)                                                                                                                       cAGTTCACGGGGAAATAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAgcg              <  2:1312738/139‑1 (MQ=255)                                                                                                                                   aaaTAACATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCa  <  1:1552178/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      CGCTGAAACCAATACGCTCGATACGCAACTGGATCTGGAACGTGACTATCAGCGACTTGCCGGACGGAGCGCAGATTATCGTGAAGGTGTCAGTGCGTTCCTGGCTAAACGCTCACCGCAGTTCACGGGGAAATAGCATGATGATAAATGTGCAAACTGTGGCAGTGATTGGGAGCGGCACCATGGGGGCAGGCATTGCTGAAGTTGCTGCCAGTCATGGACACCAGGTTTTACTGTATGACATTTCTGCTGAAGCGCTGACCCGCGCA  >  NC_000913/1458917‑1459185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑