Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	152,036	C→T	V66I (GTA→ATA)	yadL ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	152,036	0	C	T	100.0%	159.1 / NA	49	V66I (GTA→ATA)	yadL	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

CATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151901‑152163                                                                                                                                        |                                                                                                                                cATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTCTTTGCGCTTTTTTATTTGGGATACCCGCACAGTCTTTGAATTTGGGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATc                                                                                                                              >  1:1386851/1‑139 (MQ=255) cATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATc                                                                                                                              >  1:127608/1‑139 (MQ=255)          ttCTCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     >  2:557441/1‑139 (MQ=255)          ttCCCTCGCATGTGGCTTGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     <  2:376238/139‑1 (MQ=255)          ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     >  1:825409/1‑139 (MQ=255)          ttCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATc                                                                                                                     >  1:376238/1‑139 (MQ=255)                      tgGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAAt                                                                                                         >  1:246379/1‑139 (MQ=255)                         cTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTc                                                                                                      <  2:1335435/139‑1 (MQ=255)                             cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGa                                                                                                  >  2:202935/1‑139 (MQ=255)                             cTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCAGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTCTTGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGa                                                                                                  <  2:1081298/139‑1 (MQ=255)                              ttGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGAt                                                                                                 <  2:825409/139‑1 (MQ=255)                                        ttATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       <  1:1466279/139‑1 (MQ=255)                                           tttGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTAc                                                                                    <  2:246379/139‑1 (MQ=255)                                                              gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCag                                                                                              <  2:622305/110‑1 (MQ=255)                                                              gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCag                                                                                              >  1:622305/1‑110 (MQ=255)                                                              gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       <  1:1415379/117‑1 (MQ=255)                                                              gATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCAtt                                                                                       >  2:1415379/1‑117 (MQ=255)                                                                 aCCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc                                                              <  1:207299/139‑1 (MQ=255)                                                                 aCCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTAccc                                                              >  1:53474/1‑139 (MQ=255)                                                                        caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                       <  1:271898/139‑1 (MQ=255)                                                                        caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                       <  1:557441/139‑1 (MQ=255)                                                                        caGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTa                                                       <  2:53474/139‑1 (MQ=255)                                                                           tCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACag                                                    >  2:1198257/1‑139 (MQ=255)                                                                              ttGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACagtag                                                 <  1:1198257/139‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCa                                                                                                                          >  1:985068/1‑59 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCACCAACAGaa                                                                                                           >  2:1128740/1‑74 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  1:1471051/1‑74 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:144085/74‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  1:1128740/74‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           >  2:144085/1‑74 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGaa                                                                                                           <  2:1471051/74‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTc                                                                                          <  1:1546519/91‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTc                                                                                          >  2:1546519/1‑91 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTat                                                                                    >  1:693459/1‑96 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTAc                                                                                    <  2:693459/97‑1 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAAc                                          >  1:201840/1‑139 (MQ=255)                                                                                     tGAGTTTGAATGTTTTTACTTTGGTCGTGGCATTGATTTCAGAAATATATATATCACCa                                                                                                                          <  2:985068/59‑1 (MQ=255)                                                                                          ttGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCa                                     <  2:201840/139‑1 (MQ=255)                                                                                                 tttttACTTTGGTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAAt                              >  2:721396/1‑139 (MQ=255)                                                                                                           ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCaga                                                                                             >  1:565983/1‑66 (MQ=255)                                                                                                           ggTCTTGGCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCaga                                                                                             <  2:565983/66‑1 (MQ=255)                                                                                                                 ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCa              >  2:1351999/1‑139 (MQ=255)                                                                                                                 ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCa              <  1:721396/139‑1 (MQ=255)                                                                                                                 ggCATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCa              >  1:248537/1‑139 (MQ=255)                                                                                                                   cATTGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATa            <  1:1351999/139‑1 (MQ=255)                                                                                                                      tGATTTCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAaga         >  1:1572930/1‑139 (MQ=255)                                                                                                                          ttCAGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGt     <  1:202935/139‑1 (MQ=255)                                                                                                                             aGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACg  >  2:1508117/1‑139 (MQ=255)                                                                                                                             aGAAATATATATATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACg  <  1:1508117/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                CATTAGCAGTTCCCTCGCATGTGGCTCGCTTGGTTAATTTTATTTGCGCTTTTTTATTGGGGATACCCGCACAGTCTTTGAATTTGAGTTTGAATGTTTTTACTTTGGTCTTGGCATTGATTTCAGAAATATATACATCACCAAAATCAACAACAGAAATGTCCTGATCAGAGTCATTTACTAATGTAGCGGTACAAGTACCCATTTCTACAGTAGTAATAACGGTCAGTCCAATACTGTCTGTTGCCGCATAAGAGAGTACG  >  NC_000913/151901‑152163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑