Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,620,207	G→A	*73* (TAG→TAA)	marB →	periplasmic mar operon regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,620,207	0	G	A	100.0%	212.5 / NA	65	*73* (TAG→TAA)	marB	periplasmic mar operon regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/34);  total (31/34)

CCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620086‑1620334                                                                                                                          |                                                                                                                                ccAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGAc                                                                                                                >  1:510234/1‑139 (MQ=255)        cGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                         <  2:1274619/139‑1 (MQ=255)           ggTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGc                                                                                                      <  2:92662/139‑1 (MQ=255)               gttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttat                                                                                                  <  1:1488483/139‑1 (MQ=255)                ttCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 >  2:1495573/1‑139 (MQ=255)                 tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatta                                                                                                >  1:1024819/1‑139 (MQ=255)                 tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 >  2:201510/1‑138 (MQ=255)                 tCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 <  1:201510/138‑1 (MQ=255)                       aTCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                          >  1:1310136/1‑139 (MQ=255)                       aTCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTcc                                                                                          >  1:1553944/1‑139 (MQ=255)                        tCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                         <  2:1355308/139‑1 (MQ=255)                                acacCCACCGTTTGATTTAAATCACATGGGTACTGGTAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                 <  2:1247633/139‑1 (MQ=255)                                acacCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                 <  2:510234/139‑1 (MQ=255)                                   cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGa                                                                                                                 <  2:159841/104‑1 (MQ=255)                                   cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGa                                                                                                                 >  1:159841/1‑104 (MQ=255)                                   cccaccGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGc                                                                              >  1:774769/1‑139 (MQ=255)                                        cGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTAc                                                                         >  1:387517/1‑139 (MQ=255)                                          tttGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGc                                                                       <  2:1553944/139‑1 (MQ=255)                                                  aaaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCaa                                                               >  1:891618/1‑139 (MQ=255)                                                  aaaTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCaa                                                               <  2:308878/139‑1 (MQ=255)                                                    aTCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGc                                                             >  2:139917/1‑139 (MQ=255)                                                       acaTGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCaa                                                          <  2:657124/139‑1 (MQ=255)                                                          tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:1310136/139‑1 (MQ=255)                                                          tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:530486/139‑1 (MQ=255)                                                          tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:891618/139‑1 (MQ=255)                                                          tGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  2:1024819/139‑1 (MQ=255)                                                              tACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACAtt                                                   >  1:1317566/1‑139 (MQ=255)                                                                   gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                         >  2:802916/1‑96 (MQ=255)                                                                   gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTccc                                                                                         <  1:802916/96‑1 (MQ=255)                                                                   gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                              >  2:1307841/1‑139 (MQ=255)                                                                   gCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGatat                                              >  1:21800/1‑139 (MQ=255)                                                                     aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                           >  2:1201245/1‑60 (MQ=255)                                                                     aGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGtt                                                                                                                           <  1:1201245/60‑1 (MQ=255)                                                                      gTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACa                                           >  1:1205508/1‑139 (MQ=255)                                                                      gTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACa                                           >  1:170815/1‑139 (MQ=255)                                                                         aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        <  2:170815/139‑1 (MQ=255)                                                                         aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        <  1:139917/139‑1 (MQ=255)                                                                         aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        >  1:1449419/1‑139 (MQ=255)                                                                         aTAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGcc                                        <  1:1495573/139‑1 (MQ=255)                                                                              tCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                   <  2:774769/139‑1 (MQ=255)                                                                                 gATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATa                                >  2:551843/1‑139 (MQ=255)                                                                                           gCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccg                      <  2:1317566/139‑1 (MQ=255)                                                                                           gCGTGCCCTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcaccg                      <  2:1449419/139‑1 (MQ=255)                                                                                                cccTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGc                                                                                                      >  2:1202608/1‑54 (MQ=255)                                                                                                cccTATTATAATCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGc                                                                                                      <  1:1202608/54‑1 (MQ=255)                                                                                                          aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                 >  1:458130/1‑65 (MQ=255)                                                                                                          aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTAc                                                                                 <  2:458130/65‑1 (MQ=255)                                                                                                          aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa       <  2:387517/139‑1 (MQ=255)                                                                                                          aTCAACACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCa       <  1:551843/139‑1 (MQ=255)                                                                                                             aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 <  2:134971/46‑1 (MQ=255)                                                                                                             aaCACGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 >  1:134971/1‑46 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                         <  2:998620/36‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCgg                                                                                                         >  1:998620/1‑36 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 <  2:1151828/44‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCttatt                                                                                                 >  1:1151828/1‑44 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                           >  1:889728/1‑50 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTc                                                                                           <  2:889728/50‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       >  2:1469612/1‑86 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATa                                                       <  1:1469612/86‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                   >  2:58219/1‑106 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTc                                   <  1:58219/106‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcac                        <  2:1589559/117‑1 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAgcac                        >  1:1589559/1‑117 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  2:1228212/1‑139 (MQ=255)                                                                                                               cacGCTATGTAATTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGAccc  >  1:857468/1‑139 (MQ=255)                                                                                                                          |                                                                                                                                CCAATGTCGTGGTTGTTCCCCCATCGCAGGAACACCCACCGTTTGATTTAAATCACATGGGTACTGGCAGTGATAAGTCGGATGCGCTCGGCGTGCCCTATTATAATCAACACGCTATGTAGTTTGTTCTGGCCCCGACATCTCGGGGCTTATTAACTTCCCACCTTTACCGCTTTACGCCACCGCAAGCCAAATACATTGATATACAGCCCGGTCATAATGAGCACCGCACCTAAAAATTGCAGACCC  >  NC_000913/1620086‑1620334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑