Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,664,096	T→C	I163T (ATA→ACA)	ynfH →	oxidoreductase, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,664,096	0	T	C	100.0%	187.5 / NA	52	I163T (ATA→ACA)	ynfH	oxidoreductase, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

AGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAATACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGTTTGTCTCTGGCGTCTATTCACT  >  NC_000913/1663969‑1664230                                                                                                                                |                                                                                                                                       aGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCg                                                                                                                             >  1:1395232/1‑139 (MQ=255)   cATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCa                                                                                                                           >  2:790327/1‑139 (MQ=255)   cATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCa                                                                                                                           >  1:1566583/1‑139 (MQ=255)      ggCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAAtt                                                                                                                        >  1:579044/1‑139 (MQ=255)          cTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGc                                                                                                                    >  2:547678/1‑139 (MQ=255)                    ttttCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcg                                                                                                          <  2:926484/139‑1 (MQ=255)                      ttCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        <  1:1034408/139‑1 (MQ=255)                      ttCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        <  1:898254/139‑1 (MQ=255)                      ttCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        <  1:100925/139‑1 (MQ=255)                      ttCGTCTGGATGATGACCTGCGTATATCAAATCGACACCGTGCCAACCTGGCATCACGGTTACACTACGCTGGCTTTCTTCCTGCCGGTATTGTTGAGCCGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        <  2:1091884/139‑1 (MQ=255)                        cGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      <  2:728309/139‑1 (MQ=255)                             ggATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACAtt                                                                                                 <  2:678427/139‑1 (MQ=255)                              gatgatGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACAttt                                                                                                <  2:1647981/139‑1 (MQ=255)                              gatgatGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTAGCGTTACAttt                                                                                                >  1:1071647/1‑139 (MQ=255)                                   tgaCCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      >  1:1152077/1‑128 (MQ=255)                                   tgaCCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      <  2:1152077/128‑1 (MQ=255)                                          cGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCa                                                                                    >  2:605218/1‑139 (MQ=255)                                                     tCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCcatcat                                                                         <  1:790327/139‑1 (MQ=255)                                                        acacCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACca                                                                                         >  2:1269153/1‑120 (MQ=255)                                                        acacCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACca                                                                                         <  1:1269153/120‑1 (MQ=255)                                                              tGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      <  2:604735/101‑1 (MQ=255)                                                              tGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      >  1:604735/1‑101 (MQ=255)                                                                      tGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTtgc                                                                                                                                  >  2:1246518/1‑65 (MQ=255)                                                                      tGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTtgc                                                                                                                                  <  1:1246518/65‑1 (MQ=255)                                                                      tGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      <  1:1657336/93‑1 (MQ=255)                                                                      tGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      >  2:1657336/1‑93 (MQ=255)                                                                                         cGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGAt                                     >  2:1418720/1‑139 (MQ=255)                                                                                              gCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        >  2:499633/1‑67 (MQ=255)                                                                                              gCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTcgcg                                                                                                        <  1:499633/67‑1 (MQ=255)                                                                                               cTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAg                                                                      >  2:524426/1‑100 (MQ=255)                                                                                               cTTTCTTCCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAg                                                                      <  1:524426/100‑1 (MQ=255)                                                                                                   cttcCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCaa                           >  1:252045/1‑139 (MQ=255)                                                                                                     tcCTGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAgg                         <  2:1071647/139‑1 (MQ=255)                                                                                                        tGACGGTATTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGttt                      >  2:1089597/1‑139 (MQ=255)                                                                                                               aTTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACAtt                                                                                                 <  2:1066666/57‑1 (MQ=255)                                                                                                               aTTGTTGAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACAtt                                                                                                 >  1:1066666/1‑57 (MQ=255)                                                                                                                 tgttgAGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGTTTGTCTCTGGc             <  2:1286370/139‑1 (MQ=255)                                                                                                                      aGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTtgtg                                               <  2:429636/100‑1 (MQ=255)                                                                                                                      aGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTtgtg                                               >  1:429636/1‑100 (MQ=255)                                                                                                                      aGCGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGTTTGTCTCTGGCGTCTa        >  2:4748/1‑139 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATAcc                                                                                          <  2:483090/55‑1 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATAcc                                                                                          >  1:483090/1‑55 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCAt                                                                                   >  2:330202/1‑62 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCAt                                                                                   <  1:330202/62‑1 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGc                                                    <  1:10878/93‑1 (MQ=255)                                                                                                                        cGGTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGc                                                    >  2:10878/1‑93 (MQ=255)                                                                                                                          gTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      <  2:273252/41‑1 (MQ=255)                                                                                                                          gTCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGtt                                                                                                      >  1:273252/1‑41 (MQ=255)                                                                                                                           tCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCcat                                                                            >  2:660704/1‑66 (MQ=255)                                                                                                                           tCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCcat                                                                            <  1:660704/66‑1 (MQ=255)                                                                                                                           tCCAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTCACATTTAATACCACCCCATTTGCCATCATTAGCGTTCTGGCGCTTATCGCTTGTGCCGGGGTGATTGTGCTGCAAAGTTTGTCTCTGGCGTCTATTCAc   >  2:1090541/1‑139 (MQ=255)                                                                                                                            ccAACACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGTTTGTCTCTGGCGTCTATTCACt  <  2:1395232/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                       AGCATGGCGCTCGGCGTCATTTTCGTCTGGATGATGACCTGCGTGTATCAAATCGACACCGTGCCAACCTGGCATAACGGTTACACTACGCTGGCTTTCTTCCTGACGGTATTGTTGAGCGGTCCAATACTTGCTGCCGCAATTCTGCGGGCAGCTCGCGTTACATTTAATACCACACCATTTGCCATCATTAGCGTTCTGGCGCTGATCGCTTGTGCCGGGGTGATTGTGCTGCAAGGTTTGTCTCTGGCGTCTATTCACT  >  NC_000913/1663969‑1664230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑