| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,709,163 | T→C | S8P (TCC→CCC) | rsxD → | SoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,709,163 | 0 | T | C | 94.7% | 125.0 / ‑0.2 | 38 | coding (22/1059 nt) | rsxD | SoxR iron‑sulfur cluster reduction factor component, putative membrane protein of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (17/19); minor base . (1/1); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.56e-01 | |||||||||||
GGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG > NC_000913/1709027‑1709294 | ggCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGcccc > 1:1012968/1‑139 (MQ=255) ccAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATa > 2:93386/1‑139 (MQ=255) agaagaACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc < 1:1654371/139‑1 (MQ=255) agaagaACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc < 1:613551/139‑1 (MQ=255) agaagaACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc < 1:1551721/139‑1 (MQ=255) ttGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGAc > 1:499356/1‑139 (MQ=255) gATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACAt > 2:8904/1‑139 (MQ=255) ccGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCCAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc > 2:837028/1‑123 (MQ=255) ccGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAAc < 1:837028/123‑1 (MQ=255) gcAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCAt < 2:1326898/139‑1 (MQ=255) cAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATc < 1:1356973/132‑1 (MQ=255) cAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATc > 2:1356973/1‑132 (MQ=255) ggCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTc < 1:132454/139‑1 (MQ=255) aTTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAg < 1:93386/139‑1 (MQ=255) ccGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTg < 2:194093/139‑1 (MQ=255) cgcTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCa > 1:1546190/1‑139 (MQ=255) cgcTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAG‑‑‑CCC‑‑‑‑‑CCCATAACCAGCGCCAGACATCGCGCATTATGCTg > 1:106941/1‑107 (MQ=255) cgcTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAG‑‑‑CCC‑‑‑‑‑CCCATAACCAGCGCCAGACATCGCGCATTATGCTg < 2:106941/107‑1 (MQ=255) aaaaaGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgc > 1:481782/1‑139 (MQ=255) ccgccCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta < 2:1528746/61‑1 (MQ=255) ccgccCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTta > 1:1528746/1‑61 (MQ=255) cgccCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACtg < 1:948618/139‑1 (MQ=255) ttGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTttggttg < 1:8904/139‑1 (MQ=255) tAAACGAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTgggg < 2:291773/139‑1 (MQ=255) gAGGACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTActc > 1:297/1‑139 (MQ=255) ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGcc > 2:1433266/1‑96 (MQ=255) ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGcc < 1:1433266/96‑1 (MQ=255) ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACtctc > 2:1111927/1‑139 (MQ=255) ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACtctc > 2:957567/1‑139 (MQ=255) ggACTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACtctc > 1:1435017/1‑139 (MQ=255) cTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa < 1:67518/62‑1 (MQ=255) cTAAATGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa > 2:67518/1‑62 (MQ=255) aTGGTATTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGa < 2:297/139‑1 (MQ=255) aTTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATcgc > 2:945040/1‑50 (MQ=255) aTTCAGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATcgc < 1:945040/50‑1 (MQ=255) aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa < 1:964563/49‑1 (MQ=255) aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCa > 2:964563/1‑49 (MQ=255) aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc < 2:649253/94‑1 (MQ=255) aGAATAGCTAGCCCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcgc > 1:649253/1‑94 (MQ=255) agctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt < 2:261484/101‑1 (MQ=255) agctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGtttt > 1:261484/1‑101 (MQ=255) agctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCg > 1:982957/1‑139 (MQ=255) ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcg > 1:990860/1‑86 (MQ=255) ctagccCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAgcg < 2:990860/86‑1 (MQ=255) | GGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAATGGTATTCAGAATAGCTAGCTCCCCTTATACCCATAACCAGCGCCAGACATCGCGCATTATGCTGTTGGTGTTGCTCGCAGCCGTGCCAGGAATCGCAGCGCAACTGTGGTTTTTTGGTTGGGGTACTCTCGTTCAGATCCTGTTGGCATCG > NC_000913/1709027‑1709294 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |