Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,740	C→T	G428G (GGC→GGT)	mdtK →	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,740	0	C	T	100.0%	90.8 / NA	29	G428G (GGC→GGT)	mdtK	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

ACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACG  >  NC_000913/1744606‑1744875                                                                                                                                       |                                                                                                                                          aCGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGa                                                                                                                                       >  1:1161115/1‑139 (MQ=255) aCGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGa                                                                                                                                       >  1:888242/1‑139 (MQ=255)  cgcgTTCTATTTTCTATATTACCTTTACGGCTTACTGGATGCTGGGCTTGCCAAGCGGCTATAGTCTGGCACTGATCATTCTGGTCGGTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTATGAc                                                                                                                                      <  2:665566/139‑1 (MQ=255)       tCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCgg                                                                                                                                 <  1:931108/139‑1 (MQ=255)        ccATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGc                                                                                                                                <  2:1609797/139‑1 (MQ=255)        ccATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGc                                                                                                                                <  2:888242/139‑1 (MQ=255)          aTTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAg                                                                                                                              <  2:1161115/139‑1 (MQ=255)                   atTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCTGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGAAGCCATTaaga                                                                                                                     <  2:960165/139‑1 (MQ=255)                          ttACGGCTTACTGGGTGCGGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGtt                                                                                                              >  2:920442/1‑139 (MQ=255)                            aCGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑Gc                                                                                                          >  1:95074/1‑139 (MQ=255)                                                   gCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTg                                                                                   >  1:89756/1‑139 (MQ=255)                                                                      gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                         <  2:960770/132‑1 (MQ=255)                                                                      gCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTTGCGCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCca                                                                         >  1:960770/1‑132 (MQ=255)                                                                           gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGa                                                           >  1:1083446/1‑139 (MQ=255)                                                                           gACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGa                                                           >  1:1065012/1‑139 (MQ=255)                                                                            aCCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAg                                                          >  1:888882/1‑139 (MQ=255)                                                                                aTCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATc                                                      >  2:1074692/1‑139 (MQ=255)                                                                                   tGGTCGTTGAATCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGTGCATCCCg                                                   <  1:1074692/139‑1 (MQ=255)                                                                                   tGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                   <  2:1083446/139‑1 (MQ=255)                                                                                   tGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCg                                                   <  1:920442/139‑1 (MQ=255)                                                                                       cGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCtaa                                               >  1:529428/1‑139 (MQ=255)                                                                                           gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGc                                                                                                    <  2:1274107/82‑1 (MQ=255)                                                                                           gAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGc                                                                                                    >  1:1274107/1‑82 (MQ=255)                                                                                              ccTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGAc                                        <  2:529428/139‑1 (MQ=255)                                                                                                       ccAGCAGGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCaa                               <  2:95074/139‑1 (MQ=255)                                                                                                           cagGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTatg                                                                                                                      <  2:1580644/50‑1 (MQ=255)                                                                                                           cagGCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTatg                                                                                                                      >  1:1580644/1‑50 (MQ=255)                                                                                                             ggCTTCTGGATAGGCTTTATTATTGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCa                         <  2:888882/139‑1 (MQ=255)                                                                                                                                    tGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTc                                                                              >  1:227140/1‑63 (MQ=255)                                                                                                                                    tGGTCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACg  <  2:619706/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                          ACGCGTTCCATTTTCTATATTACCTTTACGGCTTACTGGGTGCTGGGCTTGCCAAGCGGCTATATTCTGGCACTGACCGATCTGGTCGTTGAACCTATGGGGCCAGCAGGCTTCTGGATAGGCTTTATTATTGGCCTGACGTCGGCAGCCATTATGATGATGTT‑‑GCGTATGCGGTTCCTGCAACGTCTGCCGTCAGCCATCATTCTGCAACGAGCATCCCGCTAATAAAGACAAGGCGCAACCTTCACGGGTTGCGCCTGTATTTTTACG  >  NC_000913/1744606‑1744875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑