Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,819,569 A→G G79G (GGT→GGC)  chbA ← N,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,819,5690AG100.0% 219.6 / NA 61G79G (GGT→GGC) chbAN,N'‑diacetylchitobiose‑specific enzyme IIA component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/27);  total (34/27)

ATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACG  >  NC_000913/1819443‑1819702
                                                                                                                              |                                                                                                                                     
atcatcGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCa                                                                                                                           >  2:276149/1‑139 (MQ=255)
     cGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGcc                                                                                                                      <  1:1578252/139‑1 (MQ=255)
          ccTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCaa                                                                                                                 <  2:128684/139‑1 (MQ=255)
               tGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGt                                                                                                            <  2:605182/139‑1 (MQ=255)
                     cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCtt                                                                                                                                   >  2:1575140/1‑110 (MQ=255)
                     cAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCtt                                                                                                                                   <  1:1575140/110‑1 (MQ=255)
                           ttCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTAcc                                                                                                <  1:1245966/139‑1 (MQ=255)
                                    cTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCt                                                                                       <  2:802309/139‑1 (MQ=255)
                                              tCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             <  1:939954/139‑1 (MQ=255)
                                                 gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGATTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCa                                                                          >  2:68317/1‑139 (MQ=255)
                                                 gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCaa                                                                                                                 >  2:509085/1‑100 (MQ=255)
                                                 gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCaa                                                                                                                 <  1:509085/100‑1 (MQ=255)
                                                 gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             >  2:515107/1‑136 (MQ=255)
                                                 gTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             <  1:515107/136‑1 (MQ=255)
                                                  tAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCAt                                                                         >  2:849862/1‑139 (MQ=255)
                                                    aTCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATcc                                                                       <  1:849862/139‑1 (MQ=255)
                                                       aGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTg                                                                    >  2:1211134/1‑139 (MQ=255)
                                                                   aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                           >  2:57110/1‑88 (MQ=255)
                                                                   aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                           <  1:57110/88‑1 (MQ=255)
                                                                   aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGAt                                                              <  2:268303/133‑1 (MQ=255)
                                                                   aGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGAt                                                              >  1:268303/1‑133 (MQ=255)
                                                                               aTTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGcc                                                                                                                               >  2:1555418/1‑56 (MQ=255)
                                                                               aTTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGcc                                                                                                                               <  1:1555418/56‑1 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGTCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGa                                                                   <  1:1497345/107‑1 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGTCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGa                                                                   >  2:1497345/1‑107 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGa                                                                                             >  1:1563213/1‑81 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGa                                                                                             <  2:1563213/81‑1 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             >  1:1355264/1‑97 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATg                                                                             <  2:1355264/97‑1 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                   >  1:1476733/1‑139 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                   >  1:913645/1‑139 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                   >  2:945790/1‑139 (MQ=255)
                                                                                        tCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAAt                                   >  1:1559050/1‑139 (MQ=255)
                                                                                         ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          <  2:981499/51‑1 (MQ=255)
                                                                                         ccTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          >  1:981499/1‑51 (MQ=255)
                                                                                           tGAGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGc                                <  2:1476733/139‑1 (MQ=255)
                                                                                             aGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          <  2:1318500/47‑1 (MQ=255)
                                                                                             aGCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          >  1:1318500/1‑47 (MQ=255)
                                                                                              gCGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCt                             >  1:1602129/1‑139 (MQ=255)
                                                                                               cGTGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTg                            >  2:161403/1‑139 (MQ=255)
                                                                                                 tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          >  1:860369/1‑43 (MQ=255)
                                                                                                 tGGACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCAt                                                                                                                          <  2:860369/43‑1 (MQ=255)
                                                                                                  ggACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCtt                         >  2:1179900/1‑139 (MQ=255)
                                                                                                   gACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                      >  2:1479370/1‑61 (MQ=255)
                                                                                                   gACCAGCACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                      <  1:1479370/61‑1 (MQ=255)
                                                                                                         cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                           <  1:894138/50‑1 (MQ=255)
                                                                                                         cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGtt                                                                                                           >  2:894138/1‑50 (MQ=255)
                                                                                                         cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                      <  2:141321/55‑1 (MQ=255)
                                                                                                         cACCAGACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTc                                                                                                      >  1:141321/1‑55 (MQ=255)
                                                                                                           ccAGACTCACTTTCCTCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGttt                <  1:1751/139‑1 (MQ=255)
                                                                                                              gACTCACTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAg             >  1:918353/1‑139 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                            >  1:1336089/1‑55 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGAt                                                                                            <  2:1336089/55‑1 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGccc                              >  2:248813/1‑117 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGccc                              <  1:248813/117‑1 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCg        >  1:757430/1‑139 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCg        >  1:814992/1‑139 (MQ=255)
                                                                                                                   aCTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCg        >  1:1187490/1‑139 (MQ=255)
                                                                                                                    cTTTCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGc       <  1:945790/139‑1 (MQ=255)
                                                                                                                      ttCATCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCAt     >  1:1462610/1‑139 (MQ=255)
                                                                                                                         aTCTTGCCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACg  >  2:1637533/1‑139 (MQ=255)
                                                                                                                              |                                                                                                                                     
ATCATCGACTCCTTATGCCTTCAGTTTTTCATGAAGCTCAATTAATTCAGTAATCAGTTCACGCGCAAGCATGGACGTCATTAAATGATCCTGAGCGTGGACCAGCACCAGACTCACTTTCATCTTACCTTCGCCCGCATCGCCTTCAATCAGTTTCGTCTGTACCAGATGCGCTTCATTCAATGCCATCCGTGACTGATCCATCATGGCTTTTGCTGCGGCAAAATCGCCCTGCTTCGCCTGTTTCAGTGCCGCATACG  >  NC_000913/1819443‑1819702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑