Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,863,004 T→C R78R (CGT→CGC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,863,0040TC100.0% 300.7 / NA 83R78R (CGT→CGC) gapAglyceraldehyde‑3‑phosphate dehydrogenase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/41);  total (42/41)

CTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862873‑1863137
                                                                                                                                   |                                                                                                                                     
cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:1296204/139‑1 (MQ=255)
cTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  1:1147216/139‑1 (MQ=255)
   ttAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGc                                                                                                                               >  1:304574/1‑137 (MQ=255)
   ttAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGc                                                                                                                               <  2:304574/137‑1 (MQ=255)
             aTTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATg                                                                                                                   >  2:28284/1‑139 (MQ=255)
                aCATGGCATACATGCTGAAATTTGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGTTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGaaacgcga                                                                                                                <  2:962466/139‑6 (MQ=255)
                   tGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                <  2:954825/136‑1 (MQ=255)
                   tGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                >  1:954825/1‑136 (MQ=255)
                       aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                <  2:1338413/116‑1 (MQ=255)
                       aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCgg                                                                                                                                >  1:1338413/1‑116 (MQ=255)
                       aTACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGtt                                                                                                         >  1:1236307/1‑139 (MQ=255)
                           aTGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGgtg                                                                                                     >  1:372839/1‑139 (MQ=255)
                                gAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGAc                                                                                                >  1:1524016/1‑139 (MQ=255)
                                         cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCg                                                                                         <  2:1247720/137‑1 (MQ=255)
                                         cTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCg                                                                                         >  1:1247720/1‑137 (MQ=255)
                                           ccACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGa                                                                                     >  2:229204/1‑139 (MQ=255)
                                           ccACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGa                                                                                     >  1:1551732/1‑139 (MQ=255)
                                              cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  1:1470032/1‑139 (MQ=255)
                                              cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  2:1258936/1‑139 (MQ=255)
                                              cTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGc                                                                                  >  2:229806/1‑139 (MQ=255)
                                                cACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  1:229806/139‑1 (MQ=255)
                                                cACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  2:1570568/139‑1 (MQ=255)
                                                 aCGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAAc                                                                               >  1:1593630/1‑139 (MQ=255)
                                                     ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  2:1524016/139‑1 (MQ=255)
                                                     ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:28284/139‑1 (MQ=255)
                                                     ccGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:11999/139‑1 (MQ=255)
                                                        tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTa                                                                                                                             <  1:373207/86‑1 (MQ=255)
                                                        tttCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTa                                                                                                                             >  2:373207/1‑86 (MQ=255)
                                                          tCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGtt                                                                      <  2:1130712/139‑1 (MQ=255)
                                                                   ccGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:1515528/125‑1 (MQ=255)
                                                                   ccGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:1515528/1‑125 (MQ=255)
                                                                   ccGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCctgactga                                                             >  1:1413145/1‑139 (MQ=255)
                                                                        gAAGTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        <  2:793833/139‑1 (MQ=255)
                                                                           gTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:228253/1‑117 (MQ=255)
                                                                           gTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:228253/117‑1 (MQ=255)
                                                                           gTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  2:372839/139‑1 (MQ=255)
                                                                           gTGAAAGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTg                                                     <  1:1120662/139‑1 (MQ=255)
                                                                                aGACGGTCATCTGATCGTTTACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGa                                                          >  1:659462/1‑129 (MQ=255)
                                                                                aGACGGTCATCTGATCGTTTACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGa                                                          <  2:659462/129‑1 (MQ=255)
                                                                                aGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:317832/112‑1 (MQ=255)
                                                                                aGACGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:317832/1‑112 (MQ=255)
                                                                                   cGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGtt                                                                                             >  1:350811/1‑91 (MQ=255)
                                                                                   cGGTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGtt                                                                                             <  2:350811/91‑1 (MQ=255)
                                                                                   cGGCCATCTGATCGTTAACGATAAAGAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTaaa                                             <  1:1068245/139‑1 (MQ=255)
                                                                                    ggTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  2:532812/103‑1 (MQ=255)
                                                                                    ggTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                >  1:532812/1‑103 (MQ=255)
                                                                                     gTCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCAGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAaca                                           >  1:449391/1‑139 (MQ=255)
                                                                                      tCATCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAacac                                          >  2:1319745/1‑139 (MQ=255)
                                                                                        aTCTGATCGTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACAt                                        <  2:1236307/139‑1 (MQ=255)
                                                                                                gTTAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTg                                >  2:1098773/1‑139 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                <  2:311293/57‑1 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGa                                                                                                                >  1:311293/1‑57 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:637066/1‑94 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:637066/94‑1 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGt                              >  2:766982/1‑139 (MQ=255)
                                                                                                  tAACGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGt                              >  1:345702/1‑139 (MQ=255)
                                                                                                    acgatAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGc                            >  2:1645016/5‑139 (MQ=255)
                                                                                                    aCGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:1198944/1‑92 (MQ=255)
                                                                                                    aCGATAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:1198944/92‑1 (MQ=255)
                                                                                                       atAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaa                         <  2:1413145/138‑1 (MQ=255)
                                                                                                       atAAAAAAATCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaa                         >  2:239205/2‑139 (MQ=255)
                                                                                                         aaaaaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCgaaga                       >  2:177982/1‑139 (MQ=255)
                                                                                                           aaaaaTCCGTGTTGCCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCCACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGaaa                     <  2:1583847/139‑1 (MQ=255)
                                                                                                            aaaaTCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAg                    <  2:1470032/139‑1 (MQ=255)
                                                                                                                tCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTg                                                                             <  1:136231/78‑1 (MQ=255)
                                                                                                                tCCGTGTTACCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTg                                                                             >  2:136231/1‑78 (MQ=255)
                                                                                                                        aCCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  2:1186782/72‑1 (MQ=255)
                                                                                                                        aCCGCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  1:1186782/1‑72 (MQ=255)
                                                                                                                           gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:239205/139‑1 (MQ=255)
                                                                                                                           gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:1645016/139‑1 (MQ=255)
                                                                                                                           gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  1:1098773/139‑1 (MQ=255)
                                                                                                                           gCTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTc     <  2:1593630/139‑1 (MQ=255)
                                                                                                                            cTGAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTcc    >  2:1653259/1‑139 (MQ=255)
                                                                                                                              gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           >  2:1351776/1‑66 (MQ=255)
                                                                                                                              gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGt                                                                           <  1:1351776/66‑1 (MQ=255)
                                                                                                                              gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGATTGGTCCGt  >  1:1000056/1‑139 (MQ=255)
                                                                                                                              gAACGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGt  >  2:816316/1‑139 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                >  1:671711/1‑60 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCaa                                                                                <  2:671711/60‑1 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCctgac                                                                <  1:1560073/76‑1 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCctgac                                                                >  2:1560073/1‑76 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        >  2:1179087/1‑84 (MQ=255)
                                                                                                                               aaCGCGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGaaa                                                        <  1:1179087/84‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
CTGTTAGACGCTGATTACATGGCATACATGCTGAAATATGACTCCACTCACGGCCGTTTCGACGGTACCGTTGAAGTGAAAGACGGTCATCTGATCGTTAACGGTAAAAAAATCCGTGTTACCGCTGAACGTGATCCGGCTAACCTGAAATGGGACGAAGTTGGTGTTGACGTTGTCGCTGAAGCAACTGGTCTGTTCCTGACTGACGAAACTGCTCGTAAACACATCACCGCTGGTGCGAAGAAAGTGGTTATGACTGGTCCGT  >  NC_000913/1862873‑1863137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑