Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,215 C→T G251G (GGC→GGT)  cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,2150CT98.6% 222.1 / NA 70G251G (GGC→GGT) cdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (33/36);  minor base G (0/1);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTG  >  NC_000913/183080‑183339
                                                                                                                                       |                                                                                                                            
aaCCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAAc                                                                                                                           >  2:259645/1‑139 (MQ=255)
           tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                    >  2:1625354/1‑135 (MQ=255)
           tggtgTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTa                                                                                                                    <  1:1625354/135‑1 (MQ=255)
                     aCCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGt                                                                                                      <  2:657376/139‑1 (MQ=255)
                          cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTg                                                                                                           >  2:287641/1‑129 (MQ=255)
                          cGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTg                                                                                                           <  1:287641/129‑1 (MQ=255)
                           gTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:1486115/139‑1 (MQ=255)
                           gTTGAACTCTTTTGGGCGCTGGGAAGCAGAAGATCAGCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCGGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:108516/139‑1 (MQ=255)
                            ttGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGccc                                                                                               <  1:1471662/139‑1 (MQ=255)
                                            gcTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGACAGTATTGCCCGATCCTATCGTACGGc                                                                               >  2:1280305/1‑139 (MQ=255)
                                                ggATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGaaa                                                                           <  2:1354835/139‑1 (MQ=255)
                                                          gaTCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGt                                                                                                      >  2:1012437/1‑102 (MQ=255)
                                                          gaTCATCGTAAGCGAGTTGAACAACGGATTACCCGCATGAAAGAGTACGGTCAGCTGCGTTTTCGCGTTTCACTGGGGAACTATTTTACCGGTCCTGGCAGt                                                                                                      <  1:1012437/102‑1 (MQ=255)
                                                           atcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCTCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                <  2:1063699/139‑1 (MQ=255)
                                                           atcatcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                <  2:1061153/139‑1 (MQ=255)
                                                               tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                            >  1:310240/1‑139 (MQ=255)
                                                               tcGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggtggtg                                                            >  1:450391/1‑139 (MQ=255)
                                                                  tAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               >  1:1384569/1‑85 (MQ=255)
                                                                  tAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               <  2:1384569/85‑1 (MQ=255)
                                                                  tAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGt                                                         >  2:1660924/1‑139 (MQ=255)
                                                                     gCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTaaa                                                      >  2:1448734/1‑139 (MQ=255)
                                                                        aGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAg                                                   >  1:1233226/1‑139 (MQ=255)
                                                                          ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  1:808220/92‑1 (MQ=255)
                                                                          ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  2:808220/1‑92 (MQ=255)
                                                                          ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                >  1:199093/1‑124 (MQ=255)
                                                                          ttGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGAtggt                                                                <  2:199093/124‑1 (MQ=255)
                                                                             aacaacTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               >  2:355974/1‑74 (MQ=255)
                                                                             aacaacTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               <  1:355974/74‑1 (MQ=255)
                                                                                     aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:364523/1‑81 (MQ=255)
                                                                                     aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  1:88068/81‑1 (MQ=255)
                                                                                     aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:364523/81‑1 (MQ=255)
                                                                                     aTTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  2:88068/1‑81 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTAcc                                                                                                                  >  2:1195807/1‑56 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTAcc                                                                                                                  <  1:1195807/56‑1 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:1140292/74‑1 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:1140292/1‑74 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:675414/1‑74 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:675414/74‑1 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGt                                    <  2:502494/134‑1 (MQ=255)
                                                                                            gCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGt                                    >  1:502494/1‑134 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               <  1:1306018/58‑1 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGt                                                                                                               >  2:1306018/1‑58 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGccc                                                                                               >  1:1181846/1‑74 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGccc                                                                                               <  2:1181846/74‑1 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:379172/73‑1 (MQ=255)
                                                                                             cATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:379172/1‑73 (MQ=255)
                                                                                                 aaaGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTATCTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTAtt                          <  1:1446292/139‑1 (MQ=255)
                                                                                                      gTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTAt                     <  1:1448734/139‑1 (MQ=255)
                                                                                                       tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                    >  1:1111289/1‑139 (MQ=255)
                                                                                                       tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                    >  2:1270764/1‑139 (MQ=255)
                                                                                                       tACGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATc                    <  2:262396/139‑1 (MQ=255)
                                                                                                         cGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  2:289825/1‑61 (MQ=255)
                                                                                                         cGGCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  1:289825/61‑1 (MQ=255)
                                                                                                          ggCCAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                               <  2:1007863/125‑1 (MQ=255)
                                                                                                          ggCCAGCTGCGTTTGCGCGTTTCACTGGGTAACTATTTTATCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCgctg                               >  1:1007863/1‑125 (MQ=255)
                                                                                                            ccAGCTGCGTTTTCGCGTTTCACTTGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:1239877/1‑58 (MQ=255)
                                                                                                            ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                >  1:44062/1‑58 (MQ=255)
                                                                                                            ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:1239877/58‑1 (MQ=255)
                                                                                                            ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGcc                                                                                                <  2:44062/58‑1 (MQ=255)
                                                                                                            ccAGCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGAt               <  1:259645/139‑1 (MQ=255)
                                                                                                               gCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCt             <  1:277168/138‑1 (MQ=255)
                                                                                                               gCTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCt             >  2:277168/1‑138 (MQ=255)
                                                                                                                cTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGa           >  2:190052/1‑139 (MQ=255)
                                                                                                                cTGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGa           >  2:1563968/1‑139 (MQ=255)
                                                                                                                 tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt          <  1:1563968/139‑1 (MQ=255)
                                                                                                                 tGCGTTTTCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGAt          >  1:1454492/1‑139 (MQ=255)
                                                                                                                      tttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTAc     <  2:450391/139‑1 (MQ=255)
                                                                                                                      tttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTAc     <  2:310240/139‑1 (MQ=255)
                                                                                                                      tttCGCGTTTCACTGGGTAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTAc     <  2:1454492/139‑1 (MQ=255)
                                                                                                                         cgcgTTTCACTGGGTAACTATTTTACCGGTCCTGACAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCtg  <  1:1280305/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                            
AACCGAAATGGTGGTGTTGAAACCGGCGTTGAACTCTTTTGGGCGCTGGGATGCAGAAGATCATCGTAAGCGAGTTGAACAACTGATTACCCGCATGAAAGAGTACGGCCAGCTGCGTTTTCGCGTTTCACTGGGCAACTATTTTACCGGTCCTGGCAGTATTGCCCGATCCTATCGTACGGCGAAAACGACGATGGTGGTGGGTAAACAGCGGATGCCAGAAAGTCGCTGCTATTTTTATCAGGATCTGATGTTACCTG  >  NC_000913/183080‑183339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑