Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,949,300	G→A	A408V (GCA→GTA)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,949,300	0	G	A	100.0%	187.8 / NA	58	A408V (GCA→GTA)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/31);  total (27/31)

AGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATA  >  NC_000913/1949166‑1949434                                                                                                                                       |                                                                                                                                       aggtgaACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACAcc                                                                                                                                    >  1:47260/1‑139 (MQ=255)   gtgaACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCa                                                                                                                                  >  1:767316/1‑139 (MQ=255)               tGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaa                                                                                                                      <  2:1551333/139‑1 (MQ=255)                  aTTGCCGTCAGGCCGCCTTGACCGTCGTCTTCAAACATCGGGATGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   >  1:1655470/1‑139 (MQ=255)                   ttGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAAt                                                                                                                  >  1:1143839/1‑139 (MQ=255)                         tCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCtt                                                                                                            >  2:218693/1‑139 (MQ=255)                              ccgccTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTtgttgt                                                                                                       <  1:186631/139‑1 (MQ=255)                                        cgtcgtCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGTGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCgaa                                                                                             <  1:1376900/139‑1 (MQ=255)                                             tcTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGaaaa                                                                                        <  1:1400096/139‑1 (MQ=255)                                                  aaaCATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCata                                                                                   <  1:826383/139‑1 (MQ=255)                                                      aTCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGc                                                                               >  1:229261/1‑139 (MQ=255)                                                       tCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGcc                                                                              >  2:318309/1‑139 (MQ=255)                                                       tCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGcc                                                                              >  1:1310546/1‑139 (MQ=255)                                                        cGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCa                                                                             >  1:1478436/1‑139 (MQ=255)                                                        cGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCa                                                                             >  2:922856/1‑139 (MQ=255)                                                          ggAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATc                                                                           <  2:1143839/139‑1 (MQ=255)                                                           gAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCt                                                                          >  2:1596804/1‑139 (MQ=255)                                                             aGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTg                                                                        <  1:888061/139‑1 (MQ=255)                                                              gTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   >  2:1556388/1‑95 (MQ=255)                                                              gTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGcaacaa                                                                                                                   <  1:1556388/95‑1 (MQ=255)                                                               tCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      <  1:287355/139‑1 (MQ=255)                                                               tCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      >  1:714293/1‑139 (MQ=255)                                                                  aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  1:346926/1‑108 (MQ=255)                                                                  aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  2:346926/108‑1 (MQ=255)                                                                  aTAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCgg                                                                   <  2:47260/139‑1 (MQ=255)                                                                       ccACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              <  2:1346845/139‑1 (MQ=255)                                                                        cacaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTAc                                                             <  1:218693/139‑1 (MQ=255)                                                                         acaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGt                                                               >  2:1389597/1‑136 (MQ=255)                                                                         acaGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGt                                                               <  1:1389597/136‑1 (MQ=255)                                                                                tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  2:245118/1‑94 (MQ=255)                                                                                tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  1:245118/94‑1 (MQ=255)                                                                                tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAgg                                                     >  1:195844/1‑139 (MQ=255)                                                                                tGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAgg                                                     <  2:229261/139‑1 (MQ=255)                                                                                 gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGa                                                    >  1:1055793/1‑139 (MQ=255)                                                                                 gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAACTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      <  2:632479/121‑1 (MQ=255)                                                                                 gCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAACTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTgcg                                                                      >  1:632479/1‑121 (MQ=255)                                                                                 gCCCATTTGCTTTCGTCGGTCAGACCAAGGCCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  >  2:755421/1‑93 (MQ=255)                                                                                 gCCCATTTGCTTTCGTCGGTCAGACCAAGGCCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCa                                                                                                  <  1:755421/93‑1 (MQ=255)                                                                                    cATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGt                                                 <  2:1478436/139‑1 (MQ=255)                                                                                          cTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTGGTTGTCGGCACCGAAGGAAATCATATCGCCATCTTGCGCGGCAGTACGCTCCAGGATGTCTTCga                                           >  2:97745/1‑139 (MQ=255)                                                                                                tcgGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGAttt                                     <  1:1596804/139‑1 (MQ=255)                                                                                                             ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCg                                                                                               >  1:570656/1‑68 (MQ=255)                                                                                                             ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCg                                                                                               <  2:570656/68‑1 (MQ=255)                                                                                                             ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              >  2:115392/1‑101 (MQ=255)                                                                                                             ggTCTTTACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTa                                                              <  1:115392/101‑1 (MQ=255)                                                                                                                  ttACCCACTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCg                   >  2:1073603/1‑139 (MQ=255)                                                                                                                        aCTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCg             >  2:1258200/1‑139 (MQ=255)                                                                                                                        aCTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCg             >  2:453073/1‑139 (MQ=255)                                                                                                                         cTTTCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCgg            <  2:767316/139‑1 (MQ=255)                                                                                                                          tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGGACTTCGCTACCggg           <  2:1655470/139‑1 (MQ=255)                                                                                                                          tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCggg           <  1:922856/139‑1 (MQ=255)                                                                                                                          tttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCggg           <  2:195844/139‑1 (MQ=255)                                                                                                                           ttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGc          >  2:962005/1‑139 (MQ=255)                                                                                                                           ttCAGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGc          <  1:1073603/139‑1 (MQ=255)                                                                                                                              aGGCGCAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGt       <  2:957916/139‑1 (MQ=255)                                                                                                                                  gcAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGAt   <  1:1403795/139‑1 (MQ=255)                                                                                                                                   cAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGTGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACGTCGCTACCGGGCTGTTGATa  <  2:1322584/139‑1 (MQ=255)                                                                                                                                   cAGTACACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATa  <  1:318309/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCATCGCGTCGGCAACAATTTTCTTGTTGTCGGCACCGAAGAAAATCATATCGCCATCTTGCGCGGCAGTACGATCCAGGATGTCTTCGATGATTTCTGCATTAAGGAACTTCGCTACCGGGCTGTTGATA  >  NC_000913/1949166‑1949434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑